The determination of the effect of some 1,3,4 thiadiazole derivatives on biochemical content (Fatty Acids, Sterols, Lipophilic Vitamins) in rat liver.

Thiadiazole derivatives and its metal compounds have antibacterial, antifungal, antitumoral, antiproliferative and antioxidant properties. In the study, the effects caused by thiadiazole ligand and its metal complexes upon the fatty acids and lipophilic vitamins in livers of rats were examined. The fatty acids in liver were specified by GC while the lipophilic vitamins were specified by HPLC. It was observed that the amounts of oleic acids (18:1, n-9) and monounsaturated fatty acids (MUFA) notably increased in the Mn complex group while the amounts of arachidonic acid (20:4, n-6) notably increased in the ligand group, compared to control group. The amounts of vitamin K2, vitamin D3 and α-tocopherol considerably increased in all groups compared to control group. It was noted that the amounts of α-tocopherol were elevated in both the Mn and Cr complex groups compared to control group. However, this elevation was matching with the amount in the same groups. Nevertheless, the amount of retinol was determined to be lower in the Mn complex group compared to other groups. Accordingly, it can be considered that thanks to the utilization of toxic metals such as manganese, cadmium and chrome, unsaturated fatty acids influenced the activities of the enzymes in liver tissue, which are in charge of fatty acid chain elongation.

Biochemical evaluation of hydroxyurea derivative schiff bases in liver of rats.

In this study, it was aimed to examine the antioxidant and antihepatotoxic effects of hydroxyurea derivative Schiff bases on serum biochemical parameters (AST, ALT, LDH, urea, creatinine and total bilirubin) and antioxidant parameters (SOD, CAT, GPx, MDA). In this study, a total of 49 adult male Wistar rats was examined and they were divided into 7 equal groups. DMSO, which is diluted only with corn oil, was administered to control group. 25 mg / kg ligand, 25 mg / kg Schiff base - manganese, 25 mg / kg Schiff base-copper, 25 mg / kg Schiff base - zinc, 25 mg / kg Schiff base - nickel, 25 mg / kg Schiff base - cobalt complexes were administered to rats of experimental group subcutaneously for 15 days with three-day intervals throughout the test process. All specimens were killed by decapitation and their livers were extracted. According to the results obtained, ALT level was observed to be higher (P<0.05) in the Cu-L group compared to other groups. LDH level was observed to be higher (P<0.05) in the Cu-L and Co-L groups compared to other groups. SOD level was observed to be higher (P<0.05) in the Cu-L, Mn-L and Zn-L groups compared to other groups. MDA level was observed to be higher (P<0.05) in the Ni-L, Cu-L, Zn-L groups compared to other groups. In conclusion, it can be suggested that the determination of the pharmacological characteristics of them can be beneficial in numerous fields of application thanks to the antioxidant and hepatotoxic activities demonstrated by hydroxyurea derivative Schiff bases.