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1.
An Acad Bras Cienc ; 95(suppl 2): e20220877, 2023.
Article in English | MEDLINE | ID: mdl-38055559

ABSTRACT

Although there are reports that polyphenol resveratrol (Rsv) may cause muscle hypertrophy in basal conditions and attenuate muscle wasting in catabolic situations, its mechanism of action is still unclear. Our study evaluated the ex vivo effects of Rsv on protein metabolism and intracellular signaling in innervated (sham-operated; Sham) and 3-day sciatic denervated (Den) rat skeletal muscles. Rsv (10-4 M) reduced total proteolysis (40%) in sham muscles. Den increased total proteolysis (~40%) in muscle, which was accompanied by an increase in the activities of ubiquitin-proteasome (~3-fold) and lysosomal (100%) proteolytic systems. Rsv reduced total proteolysis (59%) in Den muscles by inhibiting the hyperactivation of ubiquitin-proteasome (50%) and lysosomal (~70%) systems. Neither Rsv nor Den altered calcium-dependent proteolysis in muscles. Mechanistically, Rsv stimulated PKA/CREB signaling in Den muscles, and PKA blockage by H89 (50µM) abolished the antiproteolytic action of the polyphenol. Rsv reduced FoxO4 phosphorylation (~60%) in both Sham and Den muscles and Akt phosphorylation (36%) in Den muscles. Rsv also caused a homeostatic effect in Den muscles by returning their protein synthesis rates to levels similar to Sham muscles. These data indicate that Rsv directly inhibits the proteolytic activity of lysosomal and ubiquitin-proteasome systems, mainly in Den muscles through, at least in part, the activation of PKA/CREB signaling.


Subject(s)
Muscle, Skeletal , Proteasome Endopeptidase Complex , Rats , Animals , Proteolysis , Proteasome Endopeptidase Complex/metabolism , Proteasome Endopeptidase Complex/pharmacology , Resveratrol/pharmacology , Muscle, Skeletal/metabolism , Rats, Wistar , Ubiquitins/metabolism , Ubiquitins/pharmacology
2.
Ann Hematol ; 100(4): 903-911, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33523291

ABSTRACT

Hyperbilirubinemia in patients with sickle cell anemia (SCA) as a result of enhanced erythrocyte destruction, lead to cholelithiasis development in a subset of patients. Evidence suggests that hyperbilirubinemia may be related to genetic variations, such as the UGT1A1 gene promoter polymorphism, which causes Gilbert syndrome (GS). Here, we aimed to determine the frequencies of UGT1A1 promoter alleles, alpha thalassemia, and ßS haplotypes and analyze their association with cholelithiasis and bilirubin levels. The UGT1A1 alleles, -3.7 kb alpha thalassemia deletion and ßS haplotypes were determined using DNA sequencing and PCR-based assays in 913 patients with SCA. The mean of total and unconjugated bilirubin and the frequency of cholelithiasis in GS patients were higher when compared to those without this condition, regardless of age (P < 0.05). Cumulative analysis demonstrated an early age-at-onset for cholelithiasis in GS genotypes (P < 0.05). Low fetal hemoglobin (HbF) levels and normal alpha thalassemia genotype were related to cholelithiasis development (P > 0.05). However, not cholelithiasis but total and unconjugated bilirubin levels were associated with ßS haplotype. These findings confirm in a large cohort that the UGT1A1 polymorphism influences cholelithiasis and hyperbilirubinemia in SCA. HbF and alpha thalassemia also appear as modulators for cholelithiasis risk.


Subject(s)
Anemia, Sickle Cell/blood , Bilirubin/blood , Cholelithiasis/etiology , Gilbert Disease/blood , Glucuronosyltransferase/physiology , Promoter Regions, Genetic/genetics , alpha-Thalassemia/blood , Adolescent , Adult , Aged , Alleles , Anemia, Sickle Cell/complications , Anemia, Sickle Cell/enzymology , Anemia, Sickle Cell/genetics , Child , Child, Preschool , Cholelithiasis/blood , Cholelithiasis/genetics , Female , Fetal Hemoglobin/analysis , Genotype , Gilbert Disease/enzymology , Gilbert Disease/genetics , Glucuronosyltransferase/genetics , Haplotypes/genetics , Hemolysis , Humans , Hyperbilirubinemia/enzymology , Hyperbilirubinemia/etiology , Hyperbilirubinemia/genetics , Male , Middle Aged , Young Adult , alpha-Thalassemia/complications , alpha-Thalassemia/enzymology , alpha-Thalassemia/genetics
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