ABSTRACT
BACKGROUND: Several indirect techniques have been used for measuring oxidative stress in sleep apnoea-hypopnoea syndrome (SAHS) patients. The purpose of this study was to find out if both, cellular or plasma oxidative stress evaluations, are good estimators to assess oxidative stress in SAHS patients before and after one month's CPAP treatment. METHODS: The study included 28 SAHS patients requiring CPAP treatment and 15 healthy control subjects. Plasma and serum oxidative stress biomarkers (lipid peroxidation, total antioxidant capacity, and the activities of glutathione peroxidase, glutathione reductase, glutathione s-transferase, catalase and superoxide dismutase) were measured using commercial kits. Cellular oxidative stress biomarkers (mitochondrial membrane potential, intracellular glutathione, superoxide anion, and hydrogen peroxide) were analysed by flow cytometry. The Wilcoxon test for paired samples was used to compare oxidative stress and clinical parameters in patients before and after treatment with CPAP. Relationships in oxidative stress markers between controls and patients were analyzed using the Mann-Whitney U test. The Spearman correlation coefficient was calculated to estimate the linear correlations between variables. RESULTS: Oxidative stress was notably decreased after CPAP. Before CPAP, SAHS severity positively correlated with hydrogen peroxide levels, while negative correlations were observed between SAHS severity and plasma TAC in patients. Also, plasma glutathione peroxidase activity negatively correlated with cellular superoxide anion, while plasma superoxide dismutase activity positively correlated with intracellular glutathione. After CPAP, plasma TAC and glutathione peroxidase activity negatively correlated with cellular hydrogen peroxide and superoxide anion. CONCLUSIONS: In conclusion, this study seems to confirm that plasma and cellular assessment reflect, in the same way, the oxidative stress status of the studied patients. Furthermore, plasma total antioxidant capacity as well as cellular hydrogen peroxide levels can be good markers for assessing oxidative stress in SAHS patients.
Subject(s)
Continuous Positive Airway Pressure , Oxidative Stress , Subarachnoid Hemorrhage/blood , Subarachnoid Hemorrhage/therapy , Adult , Antioxidants/metabolism , Biomarkers/blood , Blood Donors , Glutathione Peroxidase/blood , Glutathione Transferase/blood , Humans , Lipid Peroxidation , Male , Middle Aged , Polysomnography , Reactive Oxygen Species/metabolism , Reference Values , Superoxide Dismutase/bloodABSTRACT
OBJECTIVE: To describe an additional case of paratesticular solitary fibrous tumor. METHODS/RESULTS: A 67-year-old man presented a paratesticular mass lasting for one year. Histological examination showed a well-circumscribed lesion comprised of spindle cells proliferation without atypia, arranged in a fascicular pattern, intimately intertwining with thick collagen fibers. Tumor cells were strongly positive for vimentine and CD-34. Diagnostic criteria, clinical features and treatment of this condition are discussed. CONCLUSION: Solitary fibrous tumors are spindle cell neoplasm originally described in the pleura, but may occur in many different sites. Intrascrotal solitary fibrous tumors are uncommon and few cases have been reported.
Subject(s)
Genital Neoplasms, Male/pathology , Scrotum , Aged , Humans , MaleABSTRACT
We report the case of a 38 y old man with antiphospholipid syndrome and exceptionally extensive central vein thrombosis in the right internal jugular vein, superior vena cava, and both subclavian veins. In spite of intensive anticoagulation therapy there was only a partial response. We suggest the MR angiography be the reference standard for diagnosis in this type of patient.
