ABSTRACT
Background/Aim: As recommended by WHO, breastfeeding is the best choice and safe for infants. The formula for infants plays an imperative role in the infant's diet and remains an excellent alternative for breast milk. The milk formula for most infants has been increasingly changed with various compositions to create a similar breast milk production. This study aims to analyze and determine the chemical composition of a few milk formulas available in the Saudi Arabian market. Materials and Methods: Thirty-five milk formula samples for infants of different age categories were collected from Riyadh City and analyzed for protein, fat, carbohydrates, lactose, total solids, total non-fat solids, calcium, iron, and zinc. Among batches collected, there were 15 branded products suitable for those of age 0-6 months, five for those of age 0-12 months, four for those of age 1-3 years, and 11 for those of age 6-12 months. Results: For infants, the milk formula sample parameters investigated varied significantly (p ≤ 0.05). A significantly high protein value was 22.72% for a brand for infants with an age of 0-6 months, and the lowest was 11.31% for a brand for those of age 0-12 months. Fat content was high in a brand (26.92%) for infants of age 0-6 months and low in a brand (17.31%) for those aged 6-12 months. The high value of carbohydrates was found in a brand (60.64%) for those of age 0-6 months and a low one (44.97%) in a brand for those of age 0-12 months. The total energy, lactose, total solids, total non-fat solids, and minerals (calcium, iron, zinc) were significantly (p ≤ 0.05) varied between milk formulas at the same age. Conclusion: There were significant variations between milk formulas of the same ages. According to age groups, some nutrients were not identical to the reference values for children's food.
ABSTRACT
This study evaluated if miR-34a/SIRT1 signalling mediates the anti-hepatosteatotic effect of resveratrol (RSV) in high-fat-diet (HFD)-fed rats. Rats were divided into seven groups (n = 6/each) as control, control + miR-34a agomir negative control, HFD, HFD + miR-34a, HFD + RSV, HFD + RSV + Ex-527 (a SIRT1 inhibitor), and HFD + RSV + miR-34a agomir. After 8 weeks, RSV suppressed dyslipidemia, lowered fasting glucose and insulin levels, improved insulin sensitivity, and prevented hepatic lipid accumulation. These effects were associated with hepatic downregulation of SREBP1 and SREBP2, upregulation of PPARα, and acetylation of Nrf2 (activation) and NF-κß p65 (inhibition). Also, RSV reduced the transcription of miR-34a and increased the nuclear localisation of SIRT1 in the livers, muscles, and adipose tissues of HFD-fed rats. All these effects were prevented by EX-527 and miR-34a agmir. In conclusion, RSV prevents HFD-induced insulin resistance and hepatic steatosis by suppressing miR-34a-induced activation of SIRT1.
