ABSTRACT
Noninvasive NMR methodology has been developed to enable monitoring of 13C-labeled xenobiotics in the rat in vivo. 2,2-Dichloro-1-(2-chlorophenyl)-1-(4-chlorophenyl)-[3-13C]-propane can be detected in the liver of intact rats by in vivo 13C surface coil NMR spectroscopy after ip administration of the compound. The experiments were performed at 1.9 and 9.4 Tesla. The intrahepatic changes of the signal intensity of the labeled compound were followed as a function of time. In the days following administration, the concentration decreased and dropped to values below the detection limit after 12 days. The study demonstrates the feasibility of studies on pharmacokinetics of 13C-labeled compounds in the rat using noninvasive, in vivo surface coil NMR spectroscopy in animals. The sensitivity allows the detection of a single dose of the drug of 200 mg/kg, but can be improved.
Subject(s)
Mitotane/analogs & derivatives , Xenobiotics/pharmacokinetics , Animals , Carbon Isotopes , Female , Magnetic Resonance Spectroscopy , Mitotane/pharmacokinetics , Rats , Rats, Inbred StrainsABSTRACT
A high-performance liquid chromatographic method was developed for a rapid qualitative and quantitative analysis of p-bromophenacyl esters of red blood cell fatty acids in humans. Both free and bound fatty acids, extracted with hexane-2-propanol (3:2) from packed red blood cells were derivatized with p-bromophenacyl bromide and analysed. Ten identical samples taken from a mixed pool of packed red blood cells from healthy subjects were analysed on two different columns. The fatty acid p-bromophenacyl esters were analysed on a 10 RP-18 column with methanol-acetonitrile-0.01 M ammonium formate as mobile phase and also on a 10 RP-8 column with acetonitrile-0.01 M ammonium formate as mobile phase. The two methods gave analogous results except in total analysis time: that on a 10 RP-8 column is ca. 40% shorter. Furthermore, a quantitative analysis of a standard solution to evaluate the extraction procedure in the absence or in the presence of the red blood cell core indicated a significant difference when the core is present.
Subject(s)
Erythrocytes/analysis , Fatty Acids/blood , Adult , Chromatography, High Pressure Liquid , Ethers, Cyclic/blood , Ethers, Cyclic/chemical synthesis , Humans , Spectrophotometry, UltravioletABSTRACT
In order to obtain detection limits low enough for the analysis of nucleoside material in biological samples, a direct liquid introduction (liquid chromatographic/mass spectrometric DLI) system was upgraded by inserting a self-built desolvation chamber between the DLI probe and the ion source and by switching to microbore high-performance liquid chromatography (HPLC) on a C-18 column. The system was evaluated for the analysis of pure nucleoside and 2'-deoxynucleoside compounds in CH3OH + H2O (80/20) and for the analysis of nucleoside mixtures which were separated using 0.01 M ammonium formate + methanol (97:3) as eluant. The detection of structurally important fragment ions in the lower mass region which could not be observed before and an enhanced sensitivity are considered to be the main improvements. In combination with an appropriate clean-up procedure the system was evaluated for the DLI liquid chromatographic/mass spectrometric analysis of some nucleosides present in a human urine sample. Pseudouridine and 5,6-dihydrouridine were detected and identified.
Subject(s)
Nucleosides/urine , Chromatography, Gas , Chromatography, Liquid , Gas Chromatography-Mass Spectrometry , Humans , Mass Spectrometry , Spectrophotometry, UltravioletABSTRACT
The mould Aspergillus niger was grown in a carefully established culture medium containing one of the following test substrates: cyclopentanone, -hexanone, -heptanone, and their 2-substituted methyl derivatives. Growth curves, glucose consumption curves and curves showing the course of the oxido-reduction reaction are given.