ABSTRACT
Photophysical properties of several BODIPY-based fluorescent dyes were investigated in systems containing blood plasma biomolecules and in model system containing bovine serum albumin in terms of electronic absorption and fluorescence spectroscopy. The interaction between the investigated dyes and protein plasma components changes spectral characteristics of the dyes and leads to bathochromic and hypochromic absorption spectra shifts accompanied by changing of fluorescence intensity. The mechanism of fluorescence changing was defined in the terms of Stern-Volmer theory. It was shown that the static factor of molecular dye-biopolymers complex formation prevails at plasma protein concentration up to 1 g/l, while the higher viscosity range is characterized mainly by nonspecific fluorophore interactions. The increase of fluorescent characteristics of phenyl-substituted BODIPY in the presence of proteins caused by resonance energy transfer and change of physicochemical properties of the molecular environment of the fluorophore was shown for the first time.
Subject(s)
Fluorescent Dyes/chemistry , Porphobilinogen/analogs & derivatives , Serum Albumin/chemistry , Animals , Cattle , Fluorescence , Humans , Molecular Conformation , Porphobilinogen/chemistry , Spectrometry, Fluorescence , Spectrophotometry, UltravioletABSTRACT
There is currently an active search for effective agents to regulate the content of nitric oxide (NO) in the body. One of the ways of reducing its blood concentration may be the binding of NO by different complexions that comprise the metal complexes of porphyrins and phthalocyanines. The study was undertaken to evaluate the protective effect of synthetic metal complexes of porphyrin and phthalocyanine in acute nitrite intoxication and endotoxic shock. The findings suggest that both study samples of the synthetic metal complexes have not prevented hemolytic anemia in acute nitrite intoxication and endotoxic shock; however, they have shown potential sorption properties against nitrate ions in the blood and brain of experimental animals, which may be the subject of a further study.
