ABSTRACT
We studied the reproductive function of albino male rats after long-term exposure to wildfire smoke and the species-specific behavior of their sexually mature offspring. After the end of exposure, the mean number of spermatogonia and the relative number of Leydig cells in rat testes decreased and the index of spermatogenesis decreased. The offspring of exposed males mated with intact females demonstrated impaired cognitive abilities and disturbances of species-specific behavior. The severity of these changes depended on time interval between the end of the inhalation exposure and mating.
Subject(s)
Reproduction , Spermatogenesis , Animals , Female , Inhalation Exposure , Male , Motor Activity , RatsABSTRACT
The authors presented data of chemical analytic control of chlorine compounds level in workplace air of vinyl chloride and polyvinyl chloride production, and biomonitoring results of vinyl chloride and 1.2-dichloroethane metabolite - thiodiacetic acid urinary level in workers of this production. Findings are exceeded hygienic norms on maximal concentrations of 1.2-dichloroethane in a workshop for vinyl chloride production from 1.0 to 2.85 MACs and of vinyl chloride in a workshop for polyvinyl chloride production from 2.06 to 5.52 MACs. Urinary levels of thiodiacetic acid were assessed in workers of vinyl chloride and polyvinyl chloride production in dependence on occupation, length of service and post-contact time.
Subject(s)
Carcinogenicity Tests/methods , Hydrocarbons, Chlorinated , Occupational Exposure , Polyvinyl Chloride , Thioglycolates/urine , Workplace/classification , Adult , Chemical Industry/methods , Environmental Monitoring/methods , Environmental Monitoring/standards , Female , Humans , Hydrocarbons, Chlorinated/adverse effects , Hydrocarbons, Chlorinated/analysis , Male , Occupational Exposure/adverse effects , Occupational Exposure/analysis , Occupational Exposure/prevention & control , Occupational Health/standards , Polyvinyl Chloride/adverse effects , Polyvinyl Chloride/analysis , Russia/epidemiology , Time FactorsABSTRACT
There is considered the improvement of methodological approaches to the gas chromatographic methods- of the detection of vinyl chloride and 1,2-dichloroethane and their metabolites--chloroethanol and monochloroacetic acid in biological fluids. There were evaluated such metrological characteristics of methods, as repeatability, interlaboratoty precision, relevance and accuracy. The value of relative expanded uncertainty does not exceed 30%. There are reported optimal regimes of gas chromatographic analysis, conditions for sample preparation. The results of the contents ofthese chemical compounds and their metabolites in biological fluids from persons working in contact with chlorinated hydrocarbons are presented These techniques can be used for the detection ofthe fact of exposure to toxic substances, assessment of the level of exposure and biomonitoring.
