1.
Ukr Biokhim Zh (1978)
; 58(4): 62-6, 1986.
Article
in Russian
| MEDLINE
| ID: mdl-3739032
ABSTRACT
The gas chromatographic procedure is suggested to determine 3-methylhistidine in biological fluids. The amino acid fraction containing 3-methylhistidine is separated by ion-exchange chromatography. Amino acids are transformed into N-trifluoroacetyl-O-isobutyl esters which are analyzed by the gas chromatography instrument with micropacked columns and ionization-resonance detector. The limit of the quantitative determination of 3-methylhistidine is 50 ng per a probe.