ABSTRACT
Moyamoya syndrome is a rare chronic progressive disease of brain vessels in which certain arteries in the brain are constricted. During the disease a collateral circulation develops around the blocked vessels to compensate for the blockage, and on angiography these collateral vessels have the appearance of a "puff of smoke". Moyamoya syndrome is extremely rare disease, especially outside of Japan We describe a clinical case of a 27-year-old patient from Tomsk with Moyamoya disease. The clinical features are an acute stroke in the right middle cerebral artery with dysarthria and paralysis of VII and XII cranial nerves. The diagnosis of moyamoya is suggested by MRI-angiogram results in accordance to the diagnostic criteria.
Subject(s)
Brain Ischemia/diagnosis , Moyamoya Disease/diagnosis , Adult , Brain/pathology , Brain Ischemia/complications , Dysarthria/diagnosis , Dysarthria/etiology , Facial Nerve Diseases/diagnosis , Facial Nerve Diseases/etiology , Humans , Hypoglossal Nerve Diseases/diagnosis , Hypoglossal Nerve Diseases/etiology , Magnetic Resonance Angiography , Male , Middle Cerebral Artery/pathology , Moyamoya Disease/complications , Moyamoya Disease/surgery , Treatment OutcomeABSTRACT
13 cases of uterine carcinosarcomas have been studied by molecular-genetic method. The impairment of some genes (K-RAS gene in the first exon, TP53 in the 5th-9th exons, PTEN in the 5th and 8th exons) has been found out. The loss of heterozygosity of TP53 gene in epithelium and mesenchyme of the uterus has been detected. According to the estimation of impairment the studied tumors was divided into monoclonal and biclonal ones. This stratification could be use as prognostic criteria of uterine carcinosarcomas clinical presentation.
Subject(s)
Carcinosarcoma/genetics , Carcinosarcoma/pathology , PTEN Phosphohydrolase/genetics , Proto-Oncogene Proteins p21(ras)/genetics , Tumor Suppressor Protein p53/genetics , Uterine Neoplasms/genetics , Uterine Neoplasms/pathology , Carcinosarcoma/metabolism , Exons , Female , Humans , Loss of Heterozygosity , PTEN Phosphohydrolase/metabolism , Proto-Oncogene Proteins p21(ras)/metabolism , Retrospective Studies , Tumor Suppressor Protein p53/metabolism , Uterine Neoplasms/metabolismABSTRACT
Clinico-morphological and immunohistochemical examinations were carried out in 46 patients with carcinocarcinoma of the corpus uteri to evaluate p53, PTEN and cyclooxygenase-2 (COX-2) expression in mesenchymal and epithelial cells of tumor. p53 expression in the epithelium and that of COX-2 in both compartments appeared to be unfavorable prognosticators of survival while that of PTEN is a lesser one in significance.
Subject(s)
Biomarkers, Tumor/analysis , Carcinosarcoma/chemistry , Carcinosarcoma/pathology , Cyclooxygenase 2/analysis , PTEN Phosphohydrolase/analysis , Tumor Suppressor Protein p53/analysis , Uterine Neoplasms/chemistry , Uterine Neoplasms/pathology , Adult , Aged , Carcinosarcoma/mortality , Female , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Middle Aged , Predictive Value of Tests , Prognosis , Survival Analysis , Uterine Neoplasms/mortalityABSTRACT
Results of clinico-morphological and immunohistochemical studies of 44 cases of carcinosarcoma of the uterus are presented in an attempt to provide pathogenetic rationale for prognosticators of the disease. Immunohistochemical reactions were used to evaluate the expression of genes and mediators p53 and PTEN and cyclooxigenase (COX-2) in cells of mesenchyme and epithelium of tumor to gauge the influence of either component on its biological behavior. All patients were divided into two groups depending on whether they revealed expression of p53, PTEN and cyclooxigenase or not. The end results--overall survival and complete remission duration--were compared in both groups. No dominance of either component of carcinosarcoma was established.
Subject(s)
Biomarkers, Tumor/analysis , Carcinosarcoma/chemistry , Uterine Neoplasms/chemistry , Adolescent , Adult , Aged , Carcinosarcoma/mortality , Carcinosarcoma/pathology , Cyclooxygenase 2/analysis , Disease-Free Survival , Female , Humans , Immunohistochemistry , Middle Aged , PTEN Phosphohydrolase/analysis , Survival Analysis , Tumor Suppressor Protein p53/analysis , Uterine Neoplasms/mortality , Uterine Neoplasms/pathology , Young AdultABSTRACT
Tissue precursors and genesis of female reproductive tract carcinoma vis-a-vis its carcinomatous and sarcomatous patterns remain unknown. To determine the clonal origin of 17 female reproductive tract carcinomas, such molecular, genetic and immunohistochemical techniques as PCR-SSCP and/or denaturing gel electrophoresis for K-ras, p53 and PTEN genes; D17S786, CHRNB1, TP53, BAT26 and BAT40 microsatellites and immunostaining for p53 protein were used. Carcinomatous and sarcomatous components were studied separately. Eight tumors were assumed to be monoclonal (combination or conversion tumors), while one--of an obscure origin. Our results suggest that carcinosarcomas were characterized by chromosomal instability. Moreover, it was shown that it is necessary to combine immunohistochemical techniques with a battery of methods including genetic ones to determine clonal origin of immunologically--stained carcinosarcomas.
Subject(s)
Biomarkers, Tumor/analysis , Carcinosarcoma/chemistry , Carcinosarcoma/genetics , Genital Neoplasms, Female/chemistry , Genital Neoplasms, Female/genetics , Adult , Aged , Biomarkers, Tumor/genetics , Carcinoma/chemistry , Carcinoma/genetics , Chromosomal Instability , Electrophoresis, Agar Gel , Female , Genes, p53 , Genes, ras , Genetic Markers , Humans , Immunohistochemistry , Microsatellite Repeats , Middle Aged , PTEN Phosphohydrolase/genetics , Polymerase Chain Reaction , Polymorphism, Single-Stranded Conformational , Receptors, Nicotinic/genetics , Sarcoma/chemistry , Sarcoma/genetics , Tumor Suppressor Protein p53/geneticsSubject(s)
Empyema/therapy , Hyperbaric Oxygenation , Acute Disease , Adult , Aged , Combined Modality Therapy , Humans , Middle AgedSubject(s)
Bronchiectasis/therapy , Hyperbaric Oxygenation , Lung Abscess/therapy , Pneumonia/therapy , Adolescent , Adult , Aged , Humans , Middle AgedABSTRACT
Synthesis of carotenes by Actinomyces chrysomallus var. carotenoides was stimulated by citric, acetic, oxalacetic, fumaric, succinic, malic, alpha-ketoglutaric, tartaric, pyruvic, and propionic acids. Acetic acid acts as a precursor of carotene synthesis and also has another stimulating mechanism of action on carotenogenesis of the actinomycete. Acetic, furmaric, malic, succinic, and alpha-ketoglutaric acids stimulate cyclization of lycopene yielding beta-carotene.
Subject(s)
Acids/pharmacology , Carotenoids/biosynthesis , Streptomyces/drug effects , Culture Media , Streptomyces/metabolism , Time FactorsABSTRACT
Biosynthesis of carotenoids by a growing culture of Actinomyces chrysomallus var. carotenoides is totally inhibited by beta-ionone added at different concentrations, at various time of the cultural growth, and in various combinations with oil. The inhibition of carotenoid synthesis by beta-ionone is of a specific character since the biomass growth under the same conditions does not increase.
