ABSTRACT
We studied the effect of acute vital stress on the parameters of the hemostasis system in young and old Wistar rats. The psychotraumatic effect was simulated as a situation of emotional stress at the sight of a predator (Indian python) catching and eating a conspecific. In young rats, acute vital stress was followed by inhibition of platelet aggregation, hypercoagulation by the external pathway and final stages of plasma hemostasis, and a decrease in fibrinogen and antithrombin III levels against the background of fibrinolysis activation. In old animals, increased platelet count with reduced aggregation capacity, hypocoagulation by the internal pathway of the plasma hemostasis activation, signs of thrombinemia (a 2-fold increase in soluble fibrin-monomer complexes), an increase in antithrombin III, and inhibition of the fibrinolytic system were observed. Age-related changes in the hemostasis parameters in rats were determined before and after stress exposure.
Subject(s)
Blood Platelets/physiology , Hemostasis , Platelet Aggregation/physiology , Stress, Psychological/blood , Age Factors , Animals , Antithrombin III/metabolism , Blood Coagulation Tests , Blood Platelets/cytology , Boidae/physiology , Fibrinogen/metabolism , Male , Platelet Count , Predatory Behavior/physiology , Rats , Rats, Wistar , Stress, Psychological/physiopathologyABSTRACT
Export and accumulation of various forms of invertase (EC 3.2.1.26) in the cell wall and culture liquid of the yeast Candida utilis was investigated. It was found that the high-molecular-weight CW-form of invertase is present in the cell wall. This form is not exported into the culture liquid, and it is by a third more glycosylated than the previously described exported S-form. It was shown that one of the two liquid forms of invertase exported into the culture-the glycosylated S-form--is retained in the cell wall, while the other one--the nonglycosylated F-form--was not detected in the cell wall. Based on these results, as well as data on the distribution dynamics of the enzyme in the culture liquid and in the cell wall during different growth stages of a yeast culture, we suggested that the nonglycosylated form was exported into the culture liquid via the zone of abnormal cell wall permeability and the glycosylated forms of this enzyme (both exported and nonexported) did not use this pathway (the degree of N-glycosylation is an important factor determining the final localization of the enzyme).
Subject(s)
Candida/enzymology , Cell Wall/enzymology , Fungal Proteins/metabolism , beta-Fructofuranosidase/metabolism , Candida/ultrastructure , Cell Wall/ultrastructure , Fungal Proteins/biosynthesis , Fungal Proteins/chemistry , Glycosylation , Isoenzymes/biosynthesis , Isoenzymes/chemistry , Isoenzymes/metabolism , Kinetics , Molecular Weight , Protein Folding , Protein Transport , beta-Fructofuranosidase/biosynthesis , beta-Fructofuranosidase/chemistryABSTRACT
The paper presents an investigation into the changes in the hemostasis system of rats during extreme exercises. It has been observed that a single two-hour swimming exercise and an eight-hour imposed running in the treadbahn are accompanied by the expressed shifts in hypercoagulation with the signs of thrombinemia. On the background of the decrease in the anticoagulative and fibrinolytic activity it creates a serious threat of intravascular blood coagulation. The preliminary thirty-day course of extractum Eleutherococci eliminated the signs of intravascular blood coagulation.
Subject(s)
Adaptation, Physiological/drug effects , Disseminated Intravascular Coagulation/prevention & control , Eleutherococcus/chemistry , Hemostasis/drug effects , Physical Exertion/drug effects , Plant Extracts/therapeutic use , Animals , Disseminated Intravascular Coagulation/blood , Dose-Response Relationship, Drug , Drug Administration Schedule , Plant Extracts/administration & dosage , Plant Extracts/isolation & purification , Rats , Rats, Wistar , SwimmingABSTRACT
The influence of a chronic (30 days) administration of an eleutherococcus extract on the haemostatic system state was studied in immobilized rats. A 3-h immobilization of untreated animals is accompanied by hypercoagulation and thrombinemia signs on the background of downregulation of the anticoagulant and fibrinolytic activity, which leads to a risk of thrombosis. Preliminary 30-day course of eleutherococcus uptake prevents the immobilization-induced thrombosis in rats.
Subject(s)
Eleutherococcus/chemistry , Hemostasis/drug effects , Immobilization/adverse effects , Platelet Aggregation/drug effects , Thrombophilia/drug therapy , Animals , Plant Extracts/therapeutic use , Rats , Rats, Wistar , Thrombophilia/etiologyABSTRACT
P33 protein was isolated from the cell walls of Candida utilis. Homology between P33 and Bgl2p proteins from the cell walls of Saccharomyces cerevisiae was shown. The important role of these proteins in molecular organization of yeast cell walls was demonstrated using trypsin proteolysis and the "gene disruption" method.
Subject(s)
Candida/genetics , Cell Wall/metabolism , Fungal Proteins/genetics , Glucan Endo-1,3-beta-D-Glucosidase/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Candida/metabolism , Cloning, Molecular , Humans , Infant, Newborn , Saccharomyces cerevisiae/metabolism , Sequence HomologyABSTRACT
The air contamination content was studied of the air samples taken from the driver's cabs of different vehicles at the time of sharp braking. The air samples contained: CO (0.7-9.7 mg/m3); n-hydrocarbons + divinyl (6.4-130.0 mg/m3); aromatic hydrocarbons (3.6-26.8 mg/m3); acrylonitrile (0.5-1.0 mg/m3) H2S (0.23-0.9 mg/m3); SO2 (1.5-7.05 mg/m3).
