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Rev Cubana Med Trop ; 43(3): 156-61, 1991.
Article in Spanish | MEDLINE | ID: mdl-9768180

ABSTRACT

We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.


Subject(s)
Aedes , Dengue Virus/physiology , Virus Replication , Aedes/virology , Animals , Cell Line , Cytopathogenic Effect, Viral , Fluorescent Antibody Technique , Larva/virology , Virus Cultivation/methods
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