ABSTRACT
OBJECTIVE: This ex vivo study aims to investigate the root filling quality of warm vertical technique, single-cone technique with GuttaFlow2, Endoseal MTA and EndoSequence BC sealer as a sealer in artificial internal resorption cavity. METHODS: For this study, 40 human single-root teeth were selected. After root preparation, the roots were horizontally sectioned 7 mm from the apex. The hemisphere cavities were created on both sides, and then they were re-approximated. According to filling methods and materials, the samples were randomly as-signed to four experimental groups: I: warm vertical compaction technique (WVC), II: single-cone technique with GuttaFlow2 (GF), III: single-cone technique with Endoseal MTA sealer (EM), IV: single-cone technique with EndoSequence BC sealer (ES). Filled roots were radiographed in buccolingual and mesiodistal views to check the obturation, and stored in humid environment at the room temperature for 7 days. Each tooth was sectioned 7 mm from the root apex at the level of the previous cut, and it was then photographed un-der stereomicroscope. The photographs and radiographs of all samples were imported to an image-analysis software to calculate the percentage of sealer, gutta-percha, and voids. The results were statistically analyzed using one-way ANOVA and Kruskal-Wallis Dunn's tests. RESULTS: In buccolingual view of radiographs, the WVC and EM groups showed significantly lower percent-age of voids (P value<0.05) compared to that in the GF group. In mesiodistal view, the WVC and EM groups showed significantly lower percentage of voids (P value<0.05) compared to that in the GF and ES groups. In stereomicroscope evaluation, the WVC and EM groups showed significantly lower percentage of voids (P value<0.05) compared to that in the GF and ES groups. The differences observed between the WVC and EM groups (P value>0.05) or between the GF and ES groups (P value>0.05) were not significant. CONCLUSION: WVC technique and single-cone technique with EM sealer are the optimum methods to fill ar-tificial resorption cavities.
ABSTRACT
Knowledge about internal anatomy plays a crucial role in the success of the root canal treatment. Many studies on internal anatomy have repeatedly reported that maxillary lateral incisors have only one canal. The primary aim of this article was to describe successful non-surgical retreatment of a permanent maxillary lateral incisor with two root canals and open apices. The treatment was carried out using dental operating microscope and the canals were obturated with mineral trioxide aggregate (MTA) as an apical plug. A review of literature was also conducted to evaluate the anatomical variations of maxillary lateral incisors.
ABSTRACT
Delayed wound healing is one of the complications of diabetes mellitus. Low-level laser therapy (LLLT) has been used to accelerate wound healing however the effect of LLLT on the hard palate wound healing in streptozotocin-induced diabetic (STZ-D) mice has not yet been characterized. This study aims to determine the effect of LLLT (He-Ne and Ga-Al-As laser) on the process of wound healing in the hard palate among diabetic and non-diabetic mice. 90 adult male mice were divided into six groups. Type 1 diabetes mellitus was induced in three groups by means of injection of STZ. Of these, one group was irradiated with He-Ne laser (DH group), one with Ga-Al-As laser (DG group) and one did not undergo any LLLT (DC group). The remaining groups were non-diabetic which were allotted to laser therapy with He-Ne laser (NH group) or with Ga-Al-As laser (NG group) or no LLLT (NC group). Five animals from each group were killed on the third, seventh, and fourteenth days after surgery, and biopsies were made for histological analysis. On the 3rd and 7th days after the surgery, the number of polymorphonuclear (PMN) cells in NH, DH, NG, and DG groups was significantly lower than that of the control groups. On the 3rd, 7th and 14th days, the fibroblasts and new blood vessel counts and collagen fibers in diabetic laser treated groups (DG and DH) were significantly higher compared to that of NC, DC, NH and NG groups. On the 7th and 14th days, the fibroblasts and new blood vessel counts and collagen fibers in NH, DH, NG, and DG groups were also significantly higher than that of the control groups, and the fibroblast and new blood vessel counts and collagen density fibers in NH and DH groups were higher than that of the NG and DG groups. LLLT with He-Ne laser compared to Ga-Al-As laser has a positive healing effect on hard palate gingival wounds in STZ-D mice.
