[Respiration and oxidative phosphorylation in isolated liver cells]. / Dykhanie i okislitel'noe fosforilirovanie v izolirovannykh kletkakh pecheni

Two methods of preparation of isolated rat liver cells were studied. In the first method, the dissociative effect of enzymes and mechanical treatment and Hanks' solution as isolation medium were used. In the second method, enzymes were replaced by compexon-ethylenediamine tetraacetate and Hanks' solution -- by 0.25 M sucrose. The cells obtained by the second method are able of oxidative phosphorylation and keep better NAD-H-cytochrom c-reductase. The fine structure of these cells revealed by means of electron microscopy analysis was well preserved.