7keto-stigmasterol and 7keto-cholesterol induce differential proteome changes to intestinal epitelial (Caco-2) cells.

Recent studies have expanded the appreciation of the roles of oxysterols triggering inflammatory, immune cytotoxic and apoptotic processes, but have not been considered for proteome analysis. A comparative proteomic study in intestinal epithelial cell cultures incubated (60 µM/24 h) with 7keto-cholesterol or 7keto-stigmasterol was performed. The influence of both compounds was studied following the nLC-TripleTOF analysis. Findings were compared to results for control cultures. In the principal component analysis (PCA) of proteome patterns, two components were extracted accounting for 99.8% of the variance in the protein expression. PCA analysis clearly discriminated between the perturbations in the proteome of cell cultures incubated with 7keto-cholesterol and 7keto-stigmasterol. These proteins participate in mitochondrial function, lipid homeostasis, inflammation and immunity and cell proliferation. Remarkable differences between proteome patterns in cell cultures exposed to 7keto-cholesterol and 7keto-stigmasterol affect macrophage migration inhibitory factor, apolipoprotein E, Bcl-2-associated transcription factor and cellular retinoic acid-binding protein. Besides, exposure to 7keto-stigmasterol increased the concentration of ubiquitin-conjugating enzyme E2 and the mitochondrial superoxide dismutase protein. Such findings raise new questions about safety studies and the regulatory potential of oxysterols in the differentiation and function of intestinal and associated immune cells, their response to environmental stimuli and impairment of absorption processes.

HEMAN, método de cribaje nutricional para pacientes hospitalarios de nuevo ingreso / HEMAN, a nutritional screening method for patients newly admitted hospital

Dada la alta prevalencia de la desnutrición hospitalaria (del 30% al 50%) se impone determinar al ingreso el estado nutricional de los pacientes. Con ello se conseguiría identificar aquellos individuos en estado de desnutrición y tratarlos, lo que permitiría acortar su estancia hospitalaria y reducir el gasto sanitario. Para ello, debemos elaborar una herramienta de cribaje nutricional de alta sensibilidad y especificidad, relacionada además a un bajo coste y rapidez. Objetivo: Validar un programa de cribaje nutricional, denominado HEMAN, que nos permita discriminar a los pacientes desnutridos al ingreso hospitalario, comparándolo con un método ya estandarizado como el NRS 2002. Métodos: Se evaluaron pacientes de nuevo ingreso de los servicios de Medicina Interna y Oncología del Hospital General Universitario de Valencia con el método HEMAN y el NRS 2002 simultáneamente. Resultados: Fueron examinados 112 pacientes diferentes, 63 hombres y 49 mujeres. El método HEMAN obtuvo 8 pacientes más en riesgo nutricional que el NRS 2002, con un porcentaje de pacientes en riesgo del 74% y 67%, respectivamente. La sensibilidad del método HEMAN fue del 100% y la especificidad del 80% respecto al NRS 2002. Conclusión: Debido a que HEMAN evalúa por separado IMC, pérdida de peso y variación en la ingesta, podemos clasificar a los pacientes desnutridos con mayor precisión y discriminar entre ellos para saber cual requiere una mayor atención nutricional, en base a los criterios de la fase III del método HEMAN (AU)

Given the high prevalence of the hospitable malnutrition (from 30 % to 50%) there is imposed to determine to the admission the nutritional status of the patients. With it one would manage to identify those individuals in condition of malnutrition and to treat them, which would allow to shorten his/her hospitable stay and to reduce the sanitary bill. For it, we must elaborate a nutritional screening tool of high sensibility and specificity, related in addition to a low cost and rapidity. Objective: To validate a screening nutritional program, called HEMAN, which allows us to discriminate against the undernourished patients to the hospitable admission, comparing it with a method already standardized as the NRS 2002. Methods: Patients evaluated to the admission of the services of Internal Medicine and Oncology of the General Universitary Hospital of Valencia with the method HEMAN and the NRS 2002 simultaneously. Results: There were examined 112 different patients, 63 men and 49 women. The method HEMAN obtained 8 more patients in nutritional risk that the NRS 2002, with a percentage of patients in risk of 74% and 67%, respectively. The sensibility of the method HEMAN was 100 % and the specificity was 80% compared to the NRS 2002. Conclusion: Due to the fact that HEMAN evaluates separately IMC, weight loss and variation of the ingestion we can classify the undernourished patients with major precision and discriminate between them to know which needs a major nutritional attention, on the basis of the criteria of the phase III of the HEMAN's method (AU)

[HEMAN, a nutritional screening method for patients newly admitted hospital]. / HEMAN, método de cribaje nutricional para pacientes hospitalarios de nuevo ingreso.

UNLABELLED: Given the high prevalence of the hospitable malnutrition (from 30 % to 50%) there is imposed to determine to the admission the nutritional status of the patients. With it one would manage to identify those individuals in condition of malnutrition and to treat them, which would allow to shorten his/her hospitable stay and to reduce the sanitary bill. For it, we must elaborate a nutritional screening tool of high sensibility and specificity, related in addition to a low cost and rapidity. OBJECTIVE: To validate a screening nutritional program, called HEMAN, which allows us to discriminate against the undernourished patients to the hospitable admission, comparing it with a method already standardized as the NRS 2002. METHODS: Patients evaluated to the admission of the services of Internal Medicine and Oncology of the General Universitary Hospital of Valencia with the method HEMAN and the NRS 2002 simultaneously. RESULTS: There were examined 112 different patients, 63 men and 49 women. The method HEMAN obtained 8 more patients in nutritional risk that the NRS 2002, with a percentage of patients in risk of 74% and 67%, respectively. The sensibility of the method HEMAN was 100 % and the specificity was 80% compared to the NRS 2002. CONCLUSION: Due to the fact that HEMAN evaluates separately IMC, weight loss and variation of the ingestion we can classify the undernourished patients with major precision and discriminate between them to know which needs a major nutritional attention, on the basis of the criteria of the phase III of the HEMAN´s method.