ABSTRACT
Aim: This research pioneers the process of obtaining information concerning the distribution and existence of seven ESBL genes linked to Pseudomonas, three virulence and five quorum sensing separated from 100 camel meat samples using PCR. Materials & methods: The Vitek system was used to identify Pseudomonas species. Phenotypic antibiotic resistance of 16 antibiotics was tested by disc diffusion. Quantification of pyocyanin, elastase, alkaline protease, biofilm and Vero cell cytotoxicity was also implemented. Results: The total number of Pseudomonas species isolated from camel meat was 10/100 identified as Pseudomonas aeruginosa 8/10, Pseudomonas fluorescens 2/10. The isolates were multidrug resistant and were resistant to four to eight antibiotics representing four to six classes. The 15 genes exhibited a huge diversity in their association. Conclusion: The results indicated that camel meat is an unpropitious hotbed for Pseudomonas species of clinical significance.
Subject(s)
Biofilms/growth & development , Drug Resistance, Multiple, Bacterial/genetics , Meat/microbiology , Pseudomonas Infections/microbiology , Pseudomonas Infections/veterinary , Pseudomonas/drug effects , Pseudomonas/genetics , Quorum Sensing/genetics , Virulence Factors/genetics , Animals , Anti-Bacterial Agents/pharmacology , Bacterial Proteins , Camelus , Chlorocebus aethiops , Disk Diffusion Antimicrobial Tests , Drug Resistance, Multiple, Bacterial/drug effects , Egypt/epidemiology , Endopeptidases , Food Microbiology , Genes, Bacterial/genetics , Pancreatic Elastase , Polymerase Chain Reaction/methods , Pseudomonas/classification , Pseudomonas/isolation & purification , Pseudomonas Infections/epidemiology , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/isolation & purification , Pseudomonas fluorescens/drug effects , Pseudomonas fluorescens/isolation & purification , Pyocyanine , Vero Cells , Virulence/genetics , beta-Lactamases/geneticsABSTRACT
BACKGROUND: In Egypt, breast cancer is estimated to be the most common cancer among females. It is also a leading cause of cancer-related mortality. Use of circulating cell-free DNA (ccf-DNA) as non-invasive biomarkers is a promising tool for diagnosis and follow-up of breast cancer (BC) patients. OBJECTIVE: To assess the role of circulating cell free DNA (nuclear and mitochondrial) in diagnosing BC. MATERIALS AND METHODS: Multiplex real time PCR was used to detect the level of ccf nuclear and mitochondrial DNA in the peripheral blood of 50 breast cancer patients together with 30 patients with benign lesions and 20 healthy controls. Laboratory investigations, histopathological staging and receptor studies were carried out for the cancer group. Receiver operating characteristic curves were used to evaluate the performance of ccf-nDNA and mtDNA. RESULTS: The levels of both nDNA and mtDNA in the cancer group were significantly higher in comparison to the benign and the healthy control group. There was a statistically significant association between nDNA and mtDNA levels and well established prognostic parameters; namely, histological grade, tumour stage, lymph node status andhormonal receptor status. CONCLUSIONS: Our data suggests that nuclear and mitochondrial ccf-DNA may be used as non-invasive biomarkers in BC.
