ABSTRACT
The Newcastle disease virus (NDV) affects wild and domesticated bird species, including commercial poultry. Although the diversity of NDV in domestic chickens is well documented, limited information is available about Newcastle disease (ND) outbreaks in other bird species. We report an annotated sequence of NDV/Vulture/Borjuri/01/22, an avirulent strain of NDV reported from Borjuri, Northeast India, in Himalayan Griffon vulture. The complete genome is 15,186 bases long with a fusion protein (F) cleavage site 112GRQGR↓L117. The phylogenetic analysis based on the F protein gene and the whole genome sequence revealed that the isolate from the vulture belongs to genotype II, sharing significant homology with vaccine strain LaSota. The study highlights the possible spillover of the virus from domestic to wild species through the food chain.
ABSTRACT
BACKGROUND: India recorded the first outbreak of African swine fever (ASF) in North-eastern region (NER) in the year 2020. AIM: The current study was undertaken to investigate the transmission of African swine fever virus (ASFV) in the wild boars of Northeast India, particularly of Assam. MATERIAL AND METHODS: ASF suspected mortal tissue remains and blood samples of wild boars collected from different locations of Assam were screened for molecular detection of swine viruses which includes Classical swine fever virus, Porcine Circovirus 2, Porcine reproductive and respiratory syndrome virus and ASFV. RESULTS: One sample each from Manas and Nameri National Parks was detected positive for ASFV. Besides this, one of the samples was positive for CSFV and one of the ASFV positive samples was also positive for PCV2. Several striking gross and microscopic alterations were noticed in different organs of ASFV infected animals. Sequencing and phylogenetic analysis of B646L gene confirmed the presence of ASFV genotype-II in wild boars. Circulation of similar genotype in domestic pigs of NER in the contemporary period as well as locations near to the aforementioned national parks indicates the transmission of ASFV from domestic to wild boars. CLINICAL RELEVANCE: The detection of ASFV in the wild boars of Assam is alarming as it is an impending threat to pig population and other endangered species (particularly Pygmy hog), making it increasingly daunting to control the disease. CONCLUSION: Chances are high for ASFV to become endemic in Assam region if stringent measures are not taken at proper time.
Subject(s)
African Swine Fever Virus , African Swine Fever , Swine Diseases , Swine , Animals , African Swine Fever Virus/genetics , African Swine Fever/epidemiology , Phylogeny , Sus scrofa , Disease Outbreaks , Swine Diseases/epidemiologyABSTRACT
Wild greater one-horned rhinos ( Rhinoceros unicornis), orphaned juveniles in human care, and orphaned calves from Kaziranga National Park, Assam, India were surveyed coprologically for gastrointestinal parasites. Parasite infections were present in 100% of wild rhino samples, 96% of orphaned juvenile samples, and 27% of orphaned calf samples. In wild rhino, observed parasite ova were primarily of trematodes Paramphistomum sp. (100%), followed by those of strongyle nematodes (94%) and the cestode Anoplocephala sp. (56%). Orphaned juvenile and calf samples were positive only for strongyles. Total fecal parasite egg counts were recorded in wild rhino (mean 64 eggs per gram [epg], range 0-270), orphan juveniles (mean 43 epg, range 0-145), and orphan calves (mean 2 epg, range 0-10). Results suggest that parasite infection in rhinos in this setting is common, though more extensive sampling would provide further information on epidemiology and potential impacts on individual health and population viability.
