The Effects of Ketamine-Propofol and Remifentanil-Propofol Combinations on Integrated Pulmonary Index During Sedation in Gastrointestinal System Endoscopy.

Objectives: Different sedo-analgesia and monitoring methods are used during endoscopic procedures. And yet, there is no consensus on optimal sedating agents. In this study, the main aim is to compare ketamine-propofol and remifentanil propofol sedo-analgesia protocols by monitoring integrated pulmonary index (IPI). Methods: The study population is divided into two groups: Group ketamine received 0.25 mg/kg ketamine and 0.75 mg/kg propofol at the beginning of anesthesia. 1 mcg/kg of remifentanil and 0.75 mg/kg propofol were administered to group remifentanil patients at the induction of anesthesia. Anesthesia maintenance was provided by titration of drug doses according to the Ramsey sedation scale. Measurements were taken at four different points in time: just before anesthesia was induced, five minutes after sedation was induced, ten minutes later, and five minutes after the treatment was finished. Results: There was no significant difference in respiratory parameters such as respiratory rate, SPO2, and EtCO2 measured in the T1 time period between the groups. In the T2 time period, a significant difference was found between the groups in the integrated pulmonary index (IPI), sPO2, respiratory rate, and systolic pressure parameters were found to be significantly higher in group ketamine. T3 time period results were higher in these three parameters: IPI, sPO2, and respiration rate. In the T2, T3, T4 time periods, there was a difference between the groups in the respiration count parameter and it was found to be higher in group ketamine. Conclusion: Although it causes slight prolongation in recovery, ketamine is a safe and effective drug that can be used during endoscopic procedures.

Finding Stability-A Case Report on the Benefits of Adapted Kata Training for Children with Autism Spectrum Disorder.

This study investigated the efficacy of an Adapted Kata Training Program (AKTP) in enhancing balance for a 10-year-old child with Autism Spectrum Disorder (ASD), employing a mixed-model approach for data collection. Over 12 weeks, the AKTP demonstrated significant improvements in the child's balance abilities, with an 11% increase in static balance, 8% in proprioceptive, 12% in horizontal, and 14% in vertical balance performance. These improvements persisted in a follow-up assessment after four weeks. Observations by the child's mother corroborated the above findings. Consequently, this research suggests the AKTP as a valuable non-pharmacological intervention to improve balance in children with ASD. However, further studies are necessary to validate these results and explore the impact on additional developmental domains, such as cognitive and motor skills.

Evaluation of murine OX40L-murine IgG1(MM1) fusion protein on immunogenicity against L. mexicana infection in BALB/c mice.

The majority of OX40L is found on professional antigen-presenting cells (APC), the potency of OX40L to enhance the immunogenicity of potential vaccines against leishmania is not yet fully investigated. There is no report of administration of OX40L on cutaneous leishmaniasis either in therapy or prophylactic immunisation and the present study for the first time reports the effect of OX40L on L. mexicana infection. In this study, B9B8E2 cells were transfected with the murine OX40L and IgG1 plasmids, were used to produce the mOX40-mIgG1 (MM1). The therapeutic effects of MM1(mOX40L-mIgG1) was tested in a challenge experiment using L. mexicana infected BALB/c mice. Mice received two doses of MM1, on day 3 and 7 after the infection. Mice receiving MM1 generated an inflammatory reaction a few days after the injection of the OX40L, which was gradually dampened and finally disappeared 3 weeks later. There was a significant delay in the growth of developing lesions in mice receiving OX40L compared to controls injected with PBS and the size of lesions in the group receiving MM1 was significantly smaller than that of injected with either PBS. 40% of mice given MM1 remained lesion free for two months, when experiments were terminated. The results clearly indicate the high therapeutic effect of mOX40L-mIgG1 fusion protein in L. mexicana infection. The effect of OX40L on the enhancement of immunisation, needs to be further investigated for developing new vaccine strategies.

Examination of the relationship between basketball referee's mental well-being and emotional regulation levels.

This study utilized a relational screening model to examine the relationship between mental well-being and emotional regulation levels among basketball referees. The research sample consisted of 327 active field referees officiating in Turkish basketball leagues during the 2021-2022 season, selected using an accessible sampling method. The sample included 13.50% (n = 44) female referees and 86.50% (n = 283) male referees, with 67.30% (n = 220) holding national accreditation and 32.70% (n = 107) being regional referees. Data collection involved a personal information form, the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS) and the Referee Emotion Regulation Scale (RERS). Statistical analyses, such as Pearson's correlation coefficient, regression analysis, t-tests, and ANOVA, were conducted using SPSS 21 software, with a significance level set at p < .05. The results of this study indicated that gender and educational status did not significantly influence mental well-being, Suppression, and Cognitive Reappraisal levels among basketball referees. However, the level of refereeing emerged as a significant variable impacting mental well-being, Suppression, and Cognitive Reappraisal levels. Notably, a positive and significant correlation was observed between mental well-being, Suppression, Cognitive Reappraisal levels, age, and experience of basketball referees. Furthermore, a positive correlation was found between referees' mental well-being and emotional regulation levels, highlighting the interplay between these factors. The findings underscore the importance of prioritizing mental well-being and emotional regulation for enhancing the performance of basketball referees. Furthermore, the study emphasizes the need to nurture these aspects to improve referees' psychological resilience and overall performance. Practical research on mental well-being and emotion regulation in refereeing can contribute significantly to the existing literature, providing valuable insights for referee training and support programs.

Understanding the Families' Perceptions of Adapted Physical Activity for Individuals with Autism Spectrum Disorder through Metaphors.

The aim of this study was to understand the perceptions, understanding and experiences of the families of individuals with autism spectrum disorder (ASD) related to adapted physical activity (APA), and their educators, through their use of metaphors. The research was based on systematic content analysis in the qualitative research model. The data were collected based on metaphors. The participants included 85 families of individuals with ASD attending private institutions operating in Istanbul and Ankara. The metaphors used by the families were examined under two headings: adapted physical activities, and educators. When the metaphors used to describe APA were examined, four themes emerged, education, emotion, support and development, and these themes were divided into the categories of guidance, skill, affection, and care. When the metaphorical perceptions of educators were examined, three themes emerged: education, emotion, and social adaptation. These were further categorized as experience, knowledge, entertainment, independence, happiness, and treatment. The metaphors showed that experiences in APA support increased self-confidence for individuals with autism spectrum disorder and support the social adaptation of individuals who have the opportunity to apply knowledge and experience. This research shows that families have positive perceptions of APA educators and their lessons.

Biological Activity of Mouse OX40L-Igg Purified With Two Novel Resins.

One of the important stimulating molecules for the function of T lymphocytes is tumor necrosis factor receptor OX40 (CD134), activated by its cognate ligand OX40L (CD134L, CD252). OX40L interactions have been proposed as a potential therapeutic target for treating infectious and non-infectious diseases. The main purpose of this study was to determine the potency of two novel resins MBI and MEP for the purification of OX40L-IgG fusion protein and the biological activities of this OX40L-IgG fusion protein. The biological activity of the OX40L-IgG purified by these resins compared with protein A sepharose resin. Mice treated with the same doses of the OX40L purified by the three resins showed a significant delay in tumor growth compared to the controls injected with PBS. Mice treated with the OX40L purified by MBI resin showed a significant delay in tumor cell (CT26) growth compared with mice injected with OX40L purified by other resins.

The Comparison of Ottawa Ankle Rules and Shetty test performances in foot-ankle trauma patients visited to the emergency department.

BACKGROUND: The Ottawa Ankle Rules (OARs) and Shetty test (ST), are assessment guidelines intended to minimize radiographs in patients with ankle trauma. The aim of this study is to determine and compare the effectiveness of OARs and ST in patients admitted to the emergency department (ED) with foot and ankle trauma. METHODS: This prospective cohort study was carried out in the ED of a tertiary care teaching hospital. OARs and ST were practiced by different doctors to patients, who were admitted with foot and ankle trauma. X-ray images were analyzed by a radiologist. Accuracy measures were covered such as sensitivity, specificity, positive predictive value, negative predictive value. RESULTS: The study was completed a total of 207 patients, after achieving the inclusion and exclusion criteria. The mean age of the patients was 33.1±16.3, and 96 (46.4%) were female. For OARs, it was determined that as sensitivity 97.22%, specificity 48.89%, positive predictive value 50.36%, negative predictive value 97.06%, positive likelihood ratio 1.9 and negative likelihood ratio 0.06. If the OARs had been used, there would have been a 32.8% reduction in the ankle X-ray system. For the ST, it was determined that as sensitivity 51.39%, specificity 85.93%, positive predictive value 66.07%, negative predictive value 76.82%, positive likelihood ratio 3.65 and negative likelihood ratio 0.57. DISCUSSION: The OARs can be used as a screening tool, due to causing the high sensitivity in foot and ankle traumas. The ST was found to be inefficient in this study. In addition, the significant reduction in the number of X-rays with the use of OARs is another major result of the study.

Paradoxical immune response in leishmaniasis: The role of toll-like receptors in disease progression.

Toll-like receptors (TLRs), members of pattern recognition receptors, are expressed on many cells of the innate immune system, and their engagements with antigens regulate specific immune responses. TLRs signalling influences species-specific immune responses during Leishmania infection; thus, TLRs play a decisive role towards elimination or exacerbation of Leishmania infection. To date, there is no single therapeutic or prophylactic approach that is fully effective against leishmaniasis. An in-depth understanding of the mechanisms by which Leishmania species evade, or exploit host immune machinery could lead to the development of novel therapeutic approaches for the prevention and management of leishmaniasis. In this review, the role of TLRs in the induction of a paradoxical immune response in leishmaniasis was discussed. This review focuses on highlighting the novel interplay of TLR2- /TLR9-driven resistance or susceptibility to 5 clinically important Leishmania species in human. The activation of TLR2/TLR9 can induce diverse anti-Leishmania activities depending on the species of infecting Leishmania parasite. Infection with L. infantum and L. mexicana initiates TLR2/9 activation leading to host protective immune response, while infection with L. major, L. donovani and L. amazonensis trigger either a TLR2- /9-related protective or non-protective immune responses. These findings suggest that TLR2 and TLR9 are targets worth pursuing either for modulation or blockage to trigger host protective immune response towards leishmaniasis.

Inhibitory Effects of Leishmania Mexicana Infection on MHC-I Expression in Bone Marrow Derived Dendritic Cells.

Background: Leishmania is a parasite causing leishmaniasis with different clinical manifestations depending on the infectious species in many countries worldwide. Although different studies have been taken place to clear the interaction of the parasite with the immune system, many aspects of immunology of leishmaniasis is remained uncertain. Methods: Bone marrow derived dendritic cells (DCs) were cultured in vitro and divided into different groups (Nottingham Trent University, Nottingham, UK). The groups were separately infected with live or autoclaved L. mexicana or loaded with Soluble Leishmania Antigen (SLA). The expression of major histocompatibility complex class I (MHC-I) molecule was checked and compared on the cultured DCs using flow cytometry. Results: Infection of L. mexicana caused a significant downregulation in expression of molecules where killed Leishmania or SLA could not induce suppression in expression of these molecules. Conclusion: L. mexicana infection results in downregulation of MHC-I expression on bone marrow-derived dendritic cells.

Non-Visual Accessibility Assessment of Videos.

Video accessibility is crucial for blind screen-reader users as online videos are increasingly playing an essential role in education, employment, and entertainment. While there exist quite a few techniques and guidelines that focus on creating accessible videos, there is a dearth of research that attempts to characterize the accessibility of existing videos. Therefore in this paper, we define and investigate a diverse set of video and audio-based accessibility features in an effort to characterize accessible and inaccessible videos. As a ground truth for our investigation, we built a custom dataset of 600 videos, in which each video was assigned an accessibility score based on the number of its wins in a Swiss-system tournament, where human annotators performed pairwise accessibility comparisons of videos. In contrast to existing accessibility research where the assessments are typically done by blind users, we recruited sighted users for our effort, since videos comprise a special case where sight could be required to better judge if any particular scene in a video is presently accessible or not. Subsequently, by examining the extent of association between the accessibility features and the accessibility scores, we could determine the features that signifcantly (positively or negatively) impact video accessibility and therefore serve as good indicators for assessing the accessibility of videos. Using the custom dataset, we also trained machine learning models that leveraged our handcrafted features to either classify an arbitrary video as accessible/inaccessible or predict an accessibility score for the video. Evaluation of our models yielded an F 1 score of 0.675 for binary classification and a mean absolute error of 0.53 for score prediction, thereby demonstrating their potential in video accessibility assessment while also illuminating their current limitations and the need for further research in this area.

Modeling Gliding-based Target Selection for Blind Touchscreen Users.

Gliding a finger on touchscreen to reach a target, that is, touch exploration, is a common selection method of blind screen-reader users. This paper investigates their gliding behavior and presents a model for their motor performance. We discovered that the gliding trajectories of blind people are a mixture of two strategies: 1) ballistic movements with iterative corrections relying on non-visual feedback, and 2) multiple sub-movements separated by stops, and concatenated until the target is reached. Based on this finding, we propose the mixture pointing model, a model that relates movement time to distance and width of the target. The model outperforms extant models, improving R2 from 0.65 for Fitts' law to 0.76, and is superior in cross-validation and information criteria. The model advances understanding of gliding-based target selection and serves as a tool for designing interface layouts for screen-reader based touch exploration.

Breaking the Accessibility Barrier in Non-Visual Interaction with PDF Forms.

PDF forms are ubiquitous. Businesses big and small, government agencies, health and educational institutions and many others have all embraced PDF forms. People use PDF forms for providing information to these entities. But people who are blind frequently find it very difficult to fill out PDF forms with screen readers, the standard assistive software that they use for interacting with computer applications. Firstly, many of the them are not even accessible as they are non-interactive and hence not editable on a computer. Secondly, even if they are interactive, it is not always easy to associate the correct labels with the form fields, either because the labels are not meaningful or the sequential reading order of the screen reader misses the visual cues that associate the correct labels with the fields. In this paper we present a solution to the accessibility problem of PDF forms. We leverage the fact that many people with visual impairments are familiar with web browsing and are proficient at filling out web forms. Thus, we create a web form layer over the PDF form via a high fidelity transformation process that attempts to preserve all the spatial relationships of the PDF elements including forms, their labels and the textual content. Blind people only interact with the web forms, and the filled out web form fields are transparently transferred to the corresponding fields in the PDF form. An optimization algorithm automatically adjusts the length and width of the PDF fields to accommodate arbitrary size field data. This ensures that the filled out PDF document does not have any truncated form-field values, and additionally, it is readable. A user study with fourteen users with visual impairments revealed that they were able to populate more form fields than the status quo and the self-reported user experience with the proposed interface was superior compared to the status quo.

A Saliency-driven Video Magnifier for People with Low Vision.

Consuming video content poses significant challenges for many screen magnifier users, which is the "go to" assistive technology for people with low vision. While screen magnifier software could be used to achieve a zoom factor that would make the content of the video visible to low-vision users, it is oftentimes a major challenge for these users to navigate through videos. Towards making videos more accessible for low-vision users, we have developed the SViM video magnifier system [6]. Specifically, SViM consists of three different magnifier interfaces with easy-to-use means of interactions. All three interfaces are driven by visual saliency as a guided signal, which provides a quantification of interestingness at the pixel-level. Saliency information, which is provided as a heatmap is then processed to obtain distinct regions of interest. These regions of interests are tracked over time and displayed using an easy-to-use interface. We present a description of our overall design and interfaces.

Ontology-Driven Transformations for PDF Form Accessibility.

Filling out PDF forms with screen readers has always been a challenge for people who are blind. Many of these forms are not interactive and hence are not accessible; even if they are interactive, the serial reading order of the screen reader makes it difficult to associate the correct labels with the form fields. This demo will present TransPAc[5], an assistive technology that enables blind people to fill out PDF forms. Since blind people are familiar with web browsing, TransPAc leverages this fact by faithfully transforming a PDF document with forms into a HTML page. The blind user fills out the form fields in the HTML page with their screen reader and these filled-in data values are transparently transferred onto the corresponding form fields in the PDF document. TransPAc thus addresses a long standing problem in PDF form accessibility.

Towards Making Videos Accessible for Low Vision Screen Magnifier Users.

People with low vision who use screen magnifiers to interact with computing devices find it very challenging to interact with dynamically changing digital content such as videos, since they do not have the luxury of time to manually move, i.e., pan the magnifier lens to different regions of interest (ROIs) or zoom into these ROIs before the content changes across frames. In this paper, we present SViM, a first of its kind screen-magnifier interface for such users that leverages advances in computer vision, particularly video saliency models, to identify salient ROIs in videos. SViM's interface allows users to zoom in/out of any point of interest, switch between ROIs via mouse clicks and provides assistive panning with the added flexibility that lets the user explore other regions of the video besides the ROIs identified by SViM. Subjective and objective evaluation of a user study with 13 low vision screen magnifier users revealed that overall the participants had a better user experience with SViM over extant screen magnifiers, indicative of the former's promise and potential for making videos accessible to low vision screen magnifier users.

SaIL: Saliency-Driven Injection of ARIA Landmarks.

Navigating webpages with screen readers is a challenge even with recent improvements in screen reader technologies and the increased adoption of web standards for accessibility, namely ARIA. ARIA landmarks, an important aspect of ARIA, lets screen reader users access different sections of the webpage quickly, by enabling them to skip over blocks of irrelevant or redundant content. However, these landmarks are sporadically and inconsistently used by web developers, and in many cases, even absent in numerous web pages. Therefore, we propose SaIL, a scalable approach that automatically detects the important sections of a web page, and then injects ARIA landmarks into the corresponding HTML markup to facilitate quick access to these sections. The central concept underlying SaIL is visual saliency, which is determined using a state-of-the-art deep learning model that was trained on gaze-tracking data collected from sighted users in the context of web browsing. We present the findings of a pilot study that demonstrated the potential of SaIL in reducing both the time and effort spent in navigating webpages with screen readers.

Molecular Identification of Free-Living Amoebae (Naegleria spp., Acanthamoeba spp. and Vermamoeba spp.) Isolated from Un-improved Hot Springs, Guilan Province, Northern Iran.

BACKGROUND: This study was conducted to determine the presence and molecular identify of Acanthamoeba, Naegleria and Vermamoeba in unimproved hot springs. METHODS: From Jul to Aug 2017, 54 water samples were collected from hot springs in different parts of the Guilan Province, North Iran. For the isolation of Acanthamoeba, Naegleria and Vermamoeba approximately 500 ml of the water samples were filtered through a cellulose nitrate membrane with a pore size of 0.45 µm. The filter was transferred onto non-nutrient agar plates seeded with Gram-negative bacteria (Escherichia coli) as a food source. The morphological key of page was used to identify free-living amoebae (FLA) using an inverted microscope, PCR amplification targeting specific genes for each genus and sequencing determined frequent species and genotypes base on NCBI database. RESULTS: Fifteen of the 54 samples were positive by culture and/or PCR for Acanthamoeba and other FLA from unimproved hot springs. By sequencing the positive isolates, the strains were shown to belong to Acanthamoeba castellanii (12 case isolates belonged to T4 genotype), 4 cases of V. vermiformis, and 3 cases of N. australiensis, 2 cases of N. pagei and 1 cases of N. gruberi. CONCLUSION: Although FLA-mediated illnesses are not as high as in environmental distribution, but because of a poor prognosis, more investigations about FLA distribution in hot springs is critical. Hot spring may enhance exposure of the amoebae in individuals. Hence, more attention to unimproved hot springs is needed to prevent free-living amoebae mediated diseases.

Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines.

Treatment of gestational trophoblastic diseases (GTD) involves surgery, radiotherapy and chemotherapy. Although, these therapeutic approaches are highly successful, drug resistance and toxicity remain a concern for high risk patients. This Chemoresistance has also been observed in the presence of cancer stem cells that are thought to be responsible for cases of cancer recurrence. In this study, we report the presence of previously unknown populations of trophoblastic stem-like cells (SLCs) that are resistant to the chemotherapeutic drug doxorubicin. We demonstrate that these populations express the stem cell markers NANOG and Sox2 and higher levels of OCT-4 (NANOG+/OCT-4high/SOX2+). Although chemoresistant, we show that the invasive capacity of these trophoblastic SLCs is significantly inhibited by doxorubicin treatment. To better characterise these populations, we also identified cellular pathways that are involved in SLCs-chemoresistance to doxorubicin. In summary, we provide evidence of the presence of NANOG+/OCT-4+/SOX2+ trophoblastic SLCs that are capable to contribute to the susceptibility to GTD and that may be involved in Chemoresistance associated with drug resistance and recurrence in high risk GTDs' patients. We propose that targeting these populations could be therapeutically exploited for clinical benefit.

CTL responses to DCs stimulated with leishmania antigens detected by DCs expressing Leishmania gp63.

BACKGROUND: Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania. DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity. OBJECTIVE: To evaluate the potency of DCs primed with soluble Leishmania mexicana antigens (SLA) in developing CTL activity. METHODS: DCs were loaded with SLA and injected to Balb/c mice. After two weeks the mice were sacrificed and their splenocytes were used as effector cells in a standard 4-hour cytotoxicity assay against DCs transfected with pcDNA3 containing L. mexicana gp63 gene. RESULTS: Immunization of Balb/c mice with DCs loaded with SLA resulted in high levels of CTL activity against DCs transfected with pcDNA3 containing L. mexicana gp63 gene. CONCLUSIONS: The results indicate a high potency for DCs primed with Leishmania antigens in inducing CTL activity, which can be used for developing an immunogenic vaccine against Leishmania.

Retracted article: Quantum dots high fluorescent signal amplification immunoassay using branched DNA and peptide nucleic acid conjugated antibody.

I, Yuan-Cheng Cao, hereby wholly retract this Analyst paper for correction. This article was submitted for publication without the knowledge and approval of the co-authors listed. Signed: Yuan-Cheng Cao, Newcastle University, UK, December 2011. This retraction is endorsed by May Copsey, Editor. Retraction published 16th December 2011.