ABSTRACT
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a complex disorder whose prevalence is rapidly growing in South America. The disturbances in the microbiota-gut-liver axis impact the liver damaging processes toward fibrosis. Gut microbiota status is shaped by dietary and lifestyle factors, depending on geographic location. We aimed to identify microbial signatures in a group of Chilean MASLD patients. Forty subjects were recruited, including healthy controls (HCs), overweight/obese subjects (Ow/Ob), patients with MASLD without fibrosis (MASLD/F-), and MASLD with fibrosis (MASLD/F+). Both MASLD and fibrosis were detected through elastography and/or biopsy, and fecal microbiota were analyzed through deep sequencing. Despite no differences in α- and ß-diversity among all groups, a higher abundance of Bilophila and a lower presence of Defluviitaleaceae, Lachnospiraceae ND3007, and Coprobacter was found in MASLD/F- and MASLD/F+, compared to HC. Ruminococcaceae UCG-013 and Sellimonas were more abundant in MASLD/F+ than in Ow/Ob; both significantly differed between MASLD/F- and MASLD/F+, compared to HC. Significant positive correlations were observed between liver stiffness and Bifidobacterium, Prevotella, Sarcina, and Acidaminococcus abundance. Our results show that MASLD is associated with changes in bacterial taxa that are known to be involved in bile acid metabolism and SCFA production, with some of them being more specifically linked to fibrosis.
Subject(s)
Gastrointestinal Microbiome , Humans , Male , Female , Middle Aged , Adult , Liver Cirrhosis/microbiology , Liver Cirrhosis/metabolism , Liver Cirrhosis/pathology , Feces/microbiology , Liver/metabolism , Liver/pathology , Fatty Liver/microbiology , Fatty Liver/metabolism , Fatty Liver/pathology , Disease Progression , Obesity/microbiology , Obesity/complications , Obesity/metabolism , Chile , Bacteria/classification , Bacteria/isolation & purification , Bacteria/genetics , Bacteria/metabolism , AgedABSTRACT
El objetivo es automatizar el sistema de corte y reconexión de líneas telefónicas apenas realizado el pago en el sistema de cobranzas bancario, mediante una red que interconecte directamente todas las centrales telefónicas digitales con un servidor central instalado en el COM, el cual distribuiria todos los procesos necesarios para el corte y reconexión de líneas telefónicas se realice en un tiempo mínimo.
