HYALURONIDASE OINTMENT IN TREATMENT OF HYPERTROPHIC SCARS.

We have studied efficiency of hyaluronidase ointment created on the base of microbial hyaluronidase preparation "Bilidase" in treatment of postoperative hypertrophic scars. A total number of 14 patients with postoperative hypertrophic scars on different part of face were enrolled in the study. All patients were treated with hyaluronidase ointment by help of iontophoresis for 10 days -1 procedure per day and than retreated 1 time with the same regimen at 4-week interval. Scar conditions were assessed in terms of pigmentation, vascularity, pliability, height, pain and pruritis in accordance with the Vancouver Scar Scale (VSS). The data of this investigation indicate that treatment of postoperative scars with hyaluronidase ointment significantly improves the function as well as a cosmetic appearance of scar tissues. Each of the investigated parameters was improved significantly after hyaluronidase treatment. Total score after 1 cycle of treatment was 5.14±0.9 vs 10±1.5 before treatment and after 2 cycles of treatment. total score was 0.85±0.9 vs 10±1.5 before treatment (p<0.001). Hyaluronidase ointment could also be recommended for preventing the scar formation in plastic surgery.

[Effects of bacteriophages on biofilm formation by strains of Pseudomonas aeruginosa].

The effects of two Pseudomonas aeruginosa bacteriophages, vB-Pa 4 and vB-Pa 5, on the formation and development of biofilms of six polyresistant hospital strains of P. aeruginosa have been investigated. Pretreatment of bacteriophages prevented the formation or almost completely prevented the growth of adequate biofilms. The biofilms that had already formed were partially or completely destroyed after phage treatment. The results demonstrate the prospects of using isolated bacteriophages of P. aeruginosa to destroy biofilms and prevent their formation.

[Investigation of metabolic paramagnetic centers in the blood of patients with prostate adenocarcinoma prior to and after the plastic orchectomy].

Paramagnetic centers in the blood of patients with prostate adenocarcinoma have been studied prior to and after plastic orchectomy by EPR. It has been shown that significant alterations in blood paramagnetic centers occur after the surgery. These alterations point to an improvement of antioxidant properties of the blood. Specifically, the content of the Fe(3+)-transferrin complexes increased, and the number of inactivated adrenoreceptors, MetHb, and Mn(2+)-containing complexes decreased. It was found that, along with improvement of the antioxidant properties of the blood, a significant decrease in the intensity of lipid peroxidation in the blood of the patients occurs.

Characteristics of morphological change in erythrocytes during metaststic adenocarcinoma of the prostate before and after castration.

The objective of our study was to establish whether there is a direct link between displayed changes in Erythrocytes and hormonal shifts talking place in organism. For the experimental research we used a blood serum and erythrocytes of 15 men with metastasized adenocarcinoma before castration and a blood serum and erythrocytes of 15 men in 6 months after castration, also a blood serum and erythrocytes of practically healthy men. Age of patients varied from 60-75 years. Structural observation of erythrocytes was conducted by means of light microscope method, and quantitative calculation of steroid hormones was performed by means of the method of imunopherment analysis. Deep morphological changes of erythrocytes were observed in peripheral blood during metastasized adenocarcinoma of prostate gland before and after castration. At the same time during this pathology we detected sharp quantitative change of steroid hormones of testicular and adrenal glands. It was established that the deep morphological changes of erythrocytes in peripheral blood should be related to the cancerous processes occurring in organism (direct way); It was established that during the noted pathology hormonal shifts taking place in organism (indirect way) influence the course of erythropoiesis and cause changes of surface architectonics of erythrocytes, as a result of which pathological erythrocytes -cells with altered form and low functional activity, are formed.

Erythrocyte membrane permeability in the men with metastatic adenocarcinoma of the prostate.

The aim of our study was to investigate the alterations of the erythrocyte membrane permeability in the men with metastatic adenocarcinoma of the prostate before and six months after castration. For experimental research were used the erythrocytes of 15 men with metastatic prostate cancer (Pca) (before and after 6 months from castration) and of the 15 practically healthy men (control group). The patients age was vacillate from 60 to 75 years old. Clinical stage of the disease was established at A. Tsulukidze National Center of Urology. Investigations revealed the changes of Na(+)/K(+)-ATP-ase activity and the changes of Na(+) and K(+) ions concentration in metastatic Pca patients before and six months after castration. It was determined that after the castration alterations of the erythrocytes transportive function is less pronounced in comparison with pre-operational data; These indicate the overall improvement of the organism's condition in the post-operational period and partial recovery of the cellular homeostasis.

Activity and content of antioxidant enzymes in prostate tumors.

AIM: To investigate the antioxidant enzyme system in blood of men with benign hyperplasia of prostate (BHP) and with prostate adenocarcinoma (CaP). METHODS: The spectrophotometrical methods were applied to study content and activity changes of superoxide-dismutase (SOD), catalase (CAT), ceruloplasmin (Cp), tripeptide glutathione (GSH), glutathione-peroxidase (GSH-Px), and glutathione-reductase (GR). Lipid peroxidation was evaluated using thiobarbituric acid (TBA)-test. Blood plasma and erythrocytes of men with prostate tumors served as the material for the studies; n = 15 for each group. RESULTS: SOD activity was increased in BHP (24.65 +/- 1.20 U/microl) and decreased in CaP (11.45 +/- 0.89 U/microl), CAT activity remained unaltered in BHP (12.41 +/- 0.85 mcat/ml) and was slightly declined in CaP (9.52 +/- 0.56 mcat/ml). Cp was increased in both kind of tumors, especially in CaP (54.27 +/- 7.22 mg%), as well as GSH (0.736 +/- 0.07 microM/l) and GR (0.031 +/- 0.002 microM/g.Hemogl/min). GSH-Px was sharply increased in BHP (0.67 +/- 0.05 microM/g.Hemogl/min) and reduced in CaP (0.16 +/- 0.01 microM/g.Hemogl/min). CONCLUSION: The development of BHP reflects relatively weakly on blood system as activity and content of antioxidant enzymes are not revealing marked changes in this disease. The significant changes are revealed in case of CaP, showing the reduced functional state of blood antioxidant enzyme system.

Dynamics of the protein spectrum changes in blood erythrocytes of male patients with prostate adenocarcinoma after plastic orchiectomy.

We have studied erythrocytes electrophoresis profiles in patients with prostate adenocarcinoma before and after plastic orchectomy and in healthy men to detect changes in the protein spectrum of erythrocytes. Our investigations have shown that during this pathology protein spectrum of erythrocytes structure undergoes substantial changes. This is demonstrated by losing of 35 KD molecular weight protein fraction and by appearance of 36 KD molecular weight protein fraction on the erythrocytes electrophoregram. It should be noted, that after plastic orchectomy we can see the relative normalization of erythrocyte's electrophoresis picture and it's approaching to the data of the control group (losing of 120 KD protein fraction and appearance of 48 KD protein fraction), which indicates to the normalization of the condition of the whole organism. It was detected that the synthesis of lower molecular protein fractions, on the one hand is influenced by rising of accantocytes percentage and on the other hand by the activation of the lipid oxidation process in erythrocytes membrane.

Use of bilidase for the treatment of experimental hypertrophic postburn cicatrices.

The efficiency of bilidase (hyaluronidase preparation) was studied in guinea pigs with experimental postburn cicatrices. Bilidase promoted normalization of the structure and histochemical picture of new cicatricial tissue. The drug can be used for the treatment of hypertrophic postburn cicatrices.

[Isolation and physico-chemical characteristics of Staphylococcus hyaluronidase]. / Vydelenie i fiziko-khimicheskie svoi'stva Stafilokokkovoi' gialuronidazy.

A scheme for purification of hyaluronidase from Staphylococcus aureus 0-15 that allows to produce a 550-fold-purified preparation with a high specific activity was developed. Hyaluronidase was found to consist of two molecular forms with different molecular weights. Staphylococcus hyaluronidase referred to as a complex preparation and its molecular forms have action optimum at pH 5.0-7.2 and at a temperature between 30 and 37 degrees C. The enzymes were highly specific to their substrate: they catalyzed the depolimerization of hyaluronic acid but did not split the other glycosaminoglycans.