Computational optical sensing and imaging 2021: feature issue introduction.

This Feature Issue includes 2 reviews and 34 research articles that highlight recent works in the field of Computational Optical Sensing and Imaging. Many of the works were presented at the 2021 OSA Topical Meeting on Computational Optical Sensing and Imaging, held virtually from July 19 to July 23, 2021. Articles in the feature issue cover a broad scope of computational imaging topics, such as microscopy, 3D imaging, phase retrieval, non-line-of-sight imaging, imaging through scattering media, ghost imaging, compressed sensing, and applications with new types of sensors. Deep learning approaches for computational imaging and sensing are also a focus of this feature issue.

Computational Optical Sensing and Imaging 2021: introduction to the feature issue.

This feature issue includes two reviews and 34 research papers that highlight recent works in the field of computational optical sensing and imaging. Many of the works were presented at the 2021 Optica (formerly OSA) Topical Meeting on Computational Optical Sensing and Imaging, held virtually from 19 July to 23 July 2021. Papers in the feature issue cover a broad scope of computational imaging topics, such as microscopy, 3D imaging, phase retrieval, non-line-of-sight imaging, imaging through scattering media, ghost imaging, compressed sensing, and applications with new types of sensors. Deep learning approaches for computational imaging and sensing are also a focus of this feature issue.

Metformin protects against diclofenac-induced toxicity in primary rat hepatocytes by preserving mitochondrial integrity via a pathway involving EPAC.

BACKGROUND AND PURPOSE: It has been shown that the antidiabetic drug metformin protects hepatocytes against toxicity by various stressors. Chronic or excessive consumption of diclofenac (DF) - a pain-relieving drug, leads to drug-induced liver injury via a mechanism involving mitochondrial damage and ultimately apoptotic death of hepatocytes. However, whether metformin protects against DF-induced toxicity is unknown. Recently, it was also shown that cAMP elevation is protective against DF-induced apoptotic death in hepatocytes, a protective effect primarily involving the downstream cAMP effector EPAC and preservation of mitochondrial function. This study therefore aimed at investigating whether metformin protects against DF-induced toxicity via cAMP-EPACs. EXPERIMENTAL APPROACH: Primary rat hepatocytes were exposed to 400 µmol/L DF. CE3F4 or ESI-O5 were used as EPAC-1 or 2 inhibitors respectively. Apoptosis was measured by caspase-3 activity and necrosis by Sytox green staining. Seahorse X96 assay was used to determine mitochondrial function. Mitochondrial reactive oxygen species (ROS) production was measured using MitoSox, mitochondrial MnSOD expression was determined by immunostaining and mitochondrial morphology (fusion and fission ratio) by 3D refractive index imaging. KEY RESULTS: Metformin (1 mmol/L) was protective against DF-induced apoptosis in hepatocytes. This protective effect was EPAC-dependent (mainly EPAC-2). Metformin restored mitochondrial morphology in an EPAC-independent manner. DF-induced mitochondrial dysfunction which was demonstrated by decreased oxygen consumption rate, an increased ROS production and a reduced MnSOD level, were all reversed by metformin in an EPAC-dependent manner. CONCLUSION AND IMPLICATIONS: Metformin protects hepatocytes against DF-induced toxicity via cAMP-dependent EPAC-2.

Tailored optical propulsion forces for controlled transport of resonant gold nanoparticles and associated thermal convective fluid flows.

Noble metal nanoparticles illuminated at their plasmonic resonance wavelength turn into heat nanosources. This phenomenon has prompted the development of numerous applications in science and technology. Simultaneous optical manipulation of such resonant nanoparticles could certainly extend the functionality and potential applications of optothermal tools. In this article, we experimentally demonstrate optical transport of single and multiple resonant nanoparticles (colloidal gold spheres of radius 200 nm) directed by tailored transverse phase-gradient forces propelling them around a 2D optical trap. We show how the phase-gradient force can be designed to efficiently change the speed of the nanoparticles. We have found that multiple hot nanoparticles assemble in the form of a quasi-stable group whose motion around the laser trap is also controlled by such optical propulsion forces. This assembly experiences a significant increase in the local temperature, which creates an optothermal convective fluid flow dragging tracer particles into the assembly. Thus, the created assembly is a moving heat source controlled by the propulsion force, enabling indirect control of fluid flows as a micro-optofluidic tool. The existence of these flows, probably caused by the temperature-induced Marangoni effect at the liquid water/superheated water interface, is confirmed by tracking free tracer particles migrating towards the assembly. We propose a straightforward method to control the assembly size, and therefore its temperature, by using a nonuniform optical propelling force that induces the splitting or merging of the group of nanoparticles. We envision further development of microscale optofluidic tools based on these achievements.

Label-free bioanalysis of Leishmania infantum using refractive index tomography with partially coherent illumination.

In this work, we report the use of refractive index (RI) tomography for quantitative analysis of unstained DH82 cell line infected with Leishmania infantum. The cell RI is reconstructed by using a modality of optical diffraction tomography technique that employs partially coherent illumination, thus enabling inherent compatibility with conventional wide-field microscopes. The experimental results demonstrate that the cell dry mass concentration (DMC) obtained from the RI allows for reliable detection and quantitative characterization of the infection and its temporal evolution. The RI provides important insight for studying morphological changes, particularly membrane blebbing linked to an apoptosis (cell death) process induced by the disease. Moreover, the results evidence that infected DH82 cells exhibit a higher DMC than healthy samples. These findings open up promising perspectives for clinical diagnosis of Leishmania.

Partially coherent illumination engineering for enhanced refractive index tomography.

Optical diffraction tomography based on partially coherent illumination (PC-ODT) is a quantitative label-free imaging technique that allows reconstructing of the object's 3D refractive index from measured through-focus intensity images. PC illumination provides advantages such as speckle noise-free imaging and inherent compatibility with conventional wide-field microscopes. Here we experimentally demonstrate that a proper design of the PC illumination, different from the familiar bright-field one, and the use of more realistic optical transfer functions (OTFs) have crucial importance in PC-ODT to significantly increase the accuracy in 3D refractive index reconstruction. While realistic OTFs properly account for the real experimental illumination conditions, the proposed PC illumination design allows for gathering the object spatial-frequency content attenuated when bright-field illumination is used.

Dynamic morphing of 3D curved laser traps for all-optical manipulation of particles.

The development of optical manipulation techniques focused on the confinement and transport of micro/nano-particles has attracted increased interest in the last decades. In particular the combination of all-optical confinement and propelling forces, respectively arising from high intensity and phase gradients of a strongly focused laser beam, is promising for optical transport. The recently developed freestyle laser trap exploits this manipulation mechanism to achieve optical transport along arbitrary 3D curves. In practice, reconfigurable 3D optical transport of numerous particles is a challenging problem because it requires the ability to easily adapt the trajectory in real time. In this work, we introduce and experimentally demonstrate a strategy for on-task adaptive design of freestyle laser traps based on a dynamic morphing technique. This provides programmable smooth transformation of the 3D shape of the curved laser trap with independent control of the propelling forces along it, that can be configured according to the considered application. Dynamic morphing, proven here on the example of colloidal dielectric micro-particles, significantly simplifies the important problem of real-time reconfigurable 3D optical transport and opens up routes for other sophisticated optical manipulation tasks.

Programmable optical transport of particles in knot circuits and networks.

A freestyle single-beam laser trap allows for multi-particle optical transport along arbitrary open or closed trajectories with independent control of the all-optical confinement and propulsion forces exerted over the particles. Here, exploiting this manipulation tool, we propose and experimentally demonstrate an optical dynamic routing technique to assist multi-particle transport in knot circuits and networks exhibiting multiple crossing paths. This new functionality for optical transport enables the particle circulation in such complex systems handling traffic jams and making possible particle separation/mixing in them. It is important for the development of programmable particle delivery and other automated optical transport operations of interest in colloidal physics, optofluidics, biophysics, etc.

Vector polymorphic beam.

A scalar polymorphic beam is designed with independent control of its intensity and phase along a strongly focused laser curve of arbitrary shape. This kind of beam has been found crucial in the creation of freestyle laser traps able to confine and drive the motion of micro/nano-particles along reconfigurable 3D trajectories in real time. Here, we present and experimentally prove the concept of vector polymorphic beam adding the benefit of independent design of the light polarization along arbitrary curves. In particular, we consider polarization shaped tangential and orthogonal to the curve that are of high interest in optical manipulation and laser micromachining. The vector polymorphic beam is described by a surprisingly simple closed-form expression and can be easily generated by using a computer generated hologram.

Optical diffraction tomography with fully and partially coherent illumination in high numerical aperture label-free microscopy [Invited].

Quantitative label-free imaging is an important tool for the study of living microorganisms that, during the last decade, has attracted wide attention from the optical community. Optical diffraction tomography (ODT) is probably the most relevant technique for quantitative label-free 3D imaging applied in wide-field microscopy in the visible range. The ODT is usually performed using spatially coherent light illumination and specially designed holographic microscopes. Nevertheless, the ODT is also compatible with partially coherent illumination and can be realized in conventional wide-field microscopes by applying refocusing techniques, as it has been recently demonstrated. Here, we compare these two ODT modalities, underlining their pros and cons and discussing the optical setups for their implementation. In particular, we pay special attention to a system that is compatible with a conventional wide-field microscope that can be used for both ODT modalities. It consists of two easily attachable modules: the first for sample illumination engineering based on digital light processing technology; the other for focus scanning by using an electrically driven tunable lens. This hardware allows for a programmable selection of the wavelength and the illumination design, and provides fast data acquisition as well. Its performance is experimentally demonstrated in the case of ODT with partially coherent illumination providing speckle-free 3D quantitative imaging.

Closed-form expression for mutual intensity evolution of Hermite-Laguerre-Gaussian Schell-model beams.

We derive a comprehensive closed-form expression for the evolution of the mutual intensity (MI) of Hermite-Laguerre-Gaussian Schell-model beams (HLG-SMBs) during propagation through rotationally symmetric optical systems. We demonstrate that the MI of the beam associated with a given HLG mode at any transverse plane can be presented as a linear superposition of the MIs of the SMBs associated with the equal and lower index modes of the same type, but of complex argument. The obtained expression allows easy analysis of the evolution of the intensity distribution and the CCF of such beams and, in particular, an understanding of the coherence singularity formation and modification during the beam propagation.

Label-free quantitative 3D tomographic imaging for partially coherent light microscopy.

Crucial benefits provided by partially coherent light microscopy such as improved spatial resolution, optical sectioning and speckle-noise suppression are exploited here to achieve 3D quantitative imaging: reconstruction of the object refractive index (RI). We present a partially coherent optical diffraction tomography technique (PC-ODT) that can be easily implemented in commercially available bright-field microscopes. We show that the high numerical apertures of the objective and condenser lenses, together with optical refocusing, are main issues for achieving fast and successful 3D RI reconstruction of weak objects. In particular, the optical refocusing is performed by a high-speed focus tunable lens mounted in front of the digital camera enabling compatibility with commercial microscopes. The technique is experimentally demonstrated on different examples: diatom cells (biosilica shells), polystyrene micro-spheres and blood cells. The results confirm the straightforward 3D-RI reconstruction of the samples providing valuable quantitative information for their analysis. Thus, the PC-ODT can be considered as an efficient and affordable alternative to coherent ODT which requires specially designed holographic microscopes.

Fast label-free microscopy technique for 3D dynamic quantitative imaging of living cells.

The refractive index (RI) is an important optical characteristic that is often exploited in label-free microscopy for analysis of biological objects. A technique for 3D RI reconstruction of living cells has to be fast enough to capture the cell dynamics and preferably needs to be compatible with standard wide-field microscopes. To solve this challenging problem, we present a technique that provides fast measurement and processing of data required for real-time 3D visualization of the object RI. Specifically, the 3D RI is reconstructed from the measurement of bright-field intensity images, axially scanned by a high-speed focus tunable lens mounted in front of a sCMOS camera, by using a direct deconvolution approach designed for partially coherent light microscopy in the non-paraxial regime. Both the measurement system and the partially coherent illumination, that provides optical sectioning and speckle-noise suppression, enable compatibility with wide-field microscopes resulting in a competitive and affordable alternative to the current holographic laser microscopes. Our experimental demonstrations show video-rate 3D RI visualization of living bacteria both freely swimming and optically manipulated by using freestyle laser traps allowing for their trapping and transport along 3D trajectories. These results prove that is possible to conduct simultaneous 4D label-free quantitative imaging and optical manipulation of living cells, which is promising for the study of the cell biophysics and biology.

Polymorphic beams and Nature inspired circuits for optical current.

Laser radiation pressure is a basis of numerous applications in science and technology such as atom cooling, particle manipulation, material processing, etc. This light force for the case of scalar beams is proportional to the intensity-weighted wavevector known as optical current. The ability to design the optical current according to the considered application brings new promising perspectives to exploit the radiation pressure. However, this is a challenging problem because it often requires confinement of the optical current within tight light curves (circuits) and adapting its local value for a particular task. Here, we present a formalism to handle this problem including its experimental demonstration. It consists of a Nature-inspired circuit shaping with independent control of the optical current provided by a new kind of beam referred to as polymorphic beam. This finding is highly relevant to diverse optical technologies and can be easily extended to electron and x-ray coherent beams.

Light-driven transport of plasmonic nanoparticles on demand.

Laser traps provide contactless manipulation of plasmonic nanoparticles (NPs) boosting the development of numerous applications in science and technology. The known trapping configurations allow immobilizing and moving single NPs or assembling them, but they are not suitable for massive optical transport of NPs along arbitrary trajectories. Here, we address this challenging problem and demonstrate that it can be handled by exploiting phase gradients forces to both confine and propel the NPs. The developed optical manipulation tool allows for programmable transport routing of NPs to around, surround or impact on objects in the host environment. An additional advantage is that the proposed confinement mechanism works for off-resonant but also resonant NPs paving the way for transport with simultaneous heating, which is of interest for targeted drug delivery and nanolithography. These findings are highly relevant to many technological applications including micro/nano-fabrication, micro-robotics and biomedicine.

Programmable simulator for beam propagation in turbulent atmosphere.

The study of light propagation though the atmosphere is crucial in different areas such as astronomy, free-space communications, remote sensing, etc. Since outdoors experiments are expensive and difficult to reproduce it is important to develop realistic numerical and experimental simulations. It has been demonstrated that spatial light modulators (SLMs) are well-suited for simulating different turbulent conditions in the laboratory. Here, we present a programmable experimental setup based on liquid crystal SLMs for simulation and analysis of the beam propagation through weak turbulent atmosphere. The simulator allows changing the propagation distances and atmospheric conditions without the need of moving optical elements. Its performance is tested for Gaussian and vortex beams.

Evolution of coherence singularities of Schell-model beams.

We show that the propagation of the widely used Schell-model partially coherent light can be easily understood using the ambiguity function. This approach is especially beneficial for the analysis of the mutual intensity of Schell-model beams (SMBs), which are associated with stable coherent beams such as Laguerre-, Hermite-, and Ince-Gaussian. We study the evolution of the coherence singularities during the SMB propagation. It is demonstrated that the distance of singularity formation depends on the coherence degree of the input beam. Moreover, it is proved that the shape, position, and number of singularity curves in far field are defined by the associated coherent beam.

Illumination coherence engineering and quantitative phase imaging.

Partially coherent illumination provides significant advantages such as speckle-free imaging and enhanced optical sectioning in optical microscopy. The knowledge of the spatial and temporal coherence is crucial to obtain accurate quantitative phase imaging (QPI) of specimens such as live cells, micrometer-sized particles, etc. In this Letter, we propose a novel technique for illumination coherence engineering. It is based on a DMD projector providing fast switchable both multi-wavelength and spatial coherence design. Its performance is experimentally demonstrated for QPI with different spatial coherence states.

Rapid quantitative phase imaging for partially coherent light microscopy.

Partially coherent light provides promising advantages for imaging applications. In contrast to its completely coherent counterpart, it prevents image degradation due to speckle noise and decreases cross-talk among the imaged objects. These facts make attractive the partially coherent illumination for accurate quantitative imaging in microscopy. In this work, we present a non-interferometric technique and system for quantitative phase imaging with simultaneous determination of the spatial coherence properties of the sample illumination. Its performance is experimentally demonstrated in several examples underlining the benefits of partial coherence for practical imagining applications. The programmable optical setup comprises an electrically tunable lens and sCMOS camera that allows for high-speed measurement in the millisecond range.

Recovery of Schell-model partially coherent beams.

Partially coherent light is often preferable to its completely coherent counterpart in applications such as imaging, sensing, and free-space optical communications. To fully exploit its advantages, techniques able to retrieve information carried by the beam are required. Here, we develop and experimentally demonstrate a phase-space optics technique for complete spatial analysis of widely used Schell-model beams. It allows for fast information recovery and can be applied for quantitative phase imaging of objects under partially coherent illumination.