Molecular detection of IL-10 level to determine severity of periodontitis in type 2 Diabetes Mellitus patients / Detecção molecular do nível de IL-10 para determinar a gravidade da periodontite em pacientes com Diabetes Mellitus tipo 2

Objective: The prevalence of periodontal disease is increasing in most countries including developing and developed countries. It affects 20-50-% of the global population. Patients with type 2 Diabetes Mellitus (DM) with severe periodontal disease had a 3.2 times higher risk of death than individuals without periodontitis. Periodontitis contributes to small-scale systemic inflammation. The objective of this study was to determine the severity of periodontitis using IL-10 (Interleukin-10) level in type 2 diabetes mellitus. Materials and Methods: This study was cross-sectional. All methods were performed following the guidelines and regulations of the Ethics Committee, Faculty of Dental Medicine, Universitas Airlangga. The samples were 90 subjects. The instruments used were questionnaires, periodontal status measurements based on Community Periodontal Index (CPI), and random blood glucose measurements. Data on the IL-10 level was obtained using Gingival Crevicular Fluid (GCF). Results: There was a significant difference in lifestyle in each group. The highest IL-10 level was found in the periodontitis group, followed by the periodontitis with the type 2 DM group. Conclusion: The level of IL-10 can be used to determine periodontitis severity in type 2 DM. Most respondents with the highest level of IL-10 were found in periodontitis followed by periodontitis with type 2 DM group. High levels of IL-10 will decrease the synthesis of Tumor Necrosis Factor Alpha (TNF-α), Interleukin-1 (IL-1), Interleukin-6 (IL-6), activation of macrophages, and Polymorphonuclear neutrophil (PMN) (AU)

Objetivo: A prevalência da doença periodontal tem aumentado na maioria dos países, incluindo países em desenvolvimento e desenvolvidos, Afetando 20-50% da população global. Pacientes com Diabetes Mellitus tipo 2 (DM) com doença periodontal grave apresentaram risco 3,2 vezes maior de morte do que indivíduos sem periodontite. O objetivo deste estudo foi determinar a gravidade da periodontite utilizando o nível de IL-10 (Interleucina-10) no diabetes mellitus tipo 2. Materiais e Métodos: Este estudo transversalfoi realizadoseguindo as orientações e regulamentos do Comitê de Ética da Faculdade de Medicina Dentária da Universitas Airlangga. Noventa participantes,responderam um questionário e foram examinados , para o estado periodontal, baseadas no Índice Periodontal Comunitário (IPC) e medidas aleatórias de glicemia. Os dados do nível de IL-10 foram obtidos utilizando Fluido Crevicular Gengival (GCF). Resultados: Houve uma diferença significativa no estilo de vida em cada grupo. O nível mais alto de IL-10 foi encontrado no grupo com periodontite, seguido pela periodontite com o grupo DM tipo 2. Conclusão: O nível de IL-10 pode ser utilizado para determinar a gravidade da periodontite no DM tipo 2. A maioria dos participantes com maior nível de IL-10 estava no grupo periodontite seguida de periodontite com DM tipo 2. Altos níveis de IL-10 diminuiem a síntese do Fator de Necrose Tumoral Alfa (TNF-α), Interleucina-1 (IL-1), Interleucina-6 (IL-6), ativação de macrófagos e neutrófilos polimorfonucleares (PMN) (AU)

Alveolar ridge preservation in post-extraction sockets using concentrated growth factors: a split-mouth, randomized, controlled clinical trial.

Aim: The aim of this clinical trial was to assess the impact of autologous concentrated growth factor (CGF) as a socket-filling material and its ridge preservation properties following the lower third molar extraction. Materials and methods: A total of 60 sides of 30 participants who had completely symmetrical bilateral impacted lower third molars were enrolled. The primary outcome variables of the study were bone height and width, bone density, and socket surface area in the coronal section. Cone beam computed tomography images were obtained immediately after surgery and three months after surgery as a temporal measure. Follow-up data were compared to the baseline using paired and unpaired t-tests. Results: CGF sites had higher values in height and width when compared to control sites (Buccal wall 32.9 ± 3.5 vs 29.4 ± 4.3 mm, Lingual wall 25.4 ± 3.5 vs 23.1 ± 4 mm, and Alveolar bone width 21.07 ± 1.55vs19.53 ± 1.90 mm, respectively). Bone density showed significantly higher values in CGF sites than in control sites (Coronal half 200 ± 127.3 vs -84.1 ± 121.3 and Apical half 406.5 ± 103 vs 64.2 ± 158.6, respectively). There was a significant difference between both sites in the reduction of the periodontal pockets. Conclusion: CGF application following surgical extraction provides an easy, low-cost, and efficient option for alveolar ridge preservation. Thus, the use of CGF by dentists during dental extractions may be encouraged, particularly when alveolar ridge preservation is required. Clinical trial registration: TCTR identification, TCTR20221028003.

Oral Health-Related Quality of Life (OHRQoL) Analysis in Partially Edentulous Patients with and without Denture Therapy.

Purpose: One of the Indonesians oral health problems today is teeth loss. Several treatments can be done to overcome the problems, especially to restore missing teeth function, which are mastication, speech function, and improve aesthetic. The aim of this study was to analyze the correlation between oral health-related quality of life (OHRQoL) domains physical health, psychological health, social relationships, environment, and the domain of Oral Impact on Daily Performance (OIDP) in partially edentulous patients with implants, conventional dentures, and non-users (without implant and conventional denture). Patients and Methods: This is a cross-sectional analytic observational study. Samples were partially edentulous patients aged 15-70 in Surabaya were taken using a simple random sampling technique within inclusion criteria. Reliability and validity analysis was carried out with Eta correlation test and then comparative analysis was performed with Kruskal Wallis and Post Hoc test with Mann Whitney U-Test. All procedures were carried out in accordance with the relevant guidelines and regulations by Ethics Committee, Faculty of Dental Medicine, Unair, Surabaya, Indonesia (No: 441/HRECC.FODM/VII/2022). Results: The results showed that there was a significant correlation between partially edentulous patients with and without dentures with the domains of physical health, psychological health, social, environment, and the domain of OIDP. Conclusion: The study showed a statistically significant correlation between OHRQoL domain of physical health, psychological health, social and environment, and the domain of OIDP in partially edentulous patients with implants, conventional dentures, and non-users (without implants and conventional dentures). Edentulism is really felt by the people and has a negative effect meaningful to the physical, economic, and psychological. For this reason, in determining the use of implants, conventional dentures, and non-users (without implants and conventional dentures) it is important to consider domains of OHRQoL consisting of physical health, psychological health, social relationships, environment, and the domain of OIDP.

Caregiver perception of children's dental caries and experience of accessing dental health services for their children / Percepção do cuidador sobre a cárie dentária infantil e experiência no acesso a serviços de saúde bucal para seus filhos

Objective: this study aims to know the caregiver's perception of children's dental caries and their experience of accessing dental health services in children. Material and Methods: this research was an analytic observational study with a cross-sectional method and used a purposive sampling technique with a sample of 56 respondents. Data were obtained through a questionnaire and the sampling technique used purposive sampling with samples were all caregivers at Cahaya Tazkia Kindergarten, Yapita Kindergarten, and Aisyiah 52 Kindergarten Surabaya. Data were analyzed using a correlation Spearman test. Results: perceived benefit and self-efficacy had a significant correlation with the experience of accessing dental health services (p-value ≤ 0.05) with coefficient correlations were 0.168 and 0.936 respectively. Meanwhile, perceived susceptibility, perceived severity, and the perceived barrier had no correlation with the experience of accessing dental health. Most male respondents in this research had a proper job to manage their children to go to dental health. Perceived susceptibility, perceived severity, perceived benefit, and self-efficacy of women caregivers in productive age were good but the perceived barrier showed a high mean. Respondents with that characteristics did not have steady jobs. Conclusion: women caregiver between the age of 21-40 years old believes that maintaining oral health is beneficial and will result in an increased number of accessing dental health services for their children. But caregivers who were women and also did not have a steady job had barriers to accessing dental health for their children (AU).

Objetivo: este estudo tem como objetivo conhecer a percepção do cuidador sobre a cárie na primeira infância e sua experiência de acesso aos serviços de saúde bucal em crianças. Material e Métodos: esta pesquisa foi um estudo observacional analítico transversal e utilizou uma técnica de amostragem intencional com uma amostra de 56 entrevistados. Os dados foram obtidos por meio de um questionário e a técnica de amostragem utilizou amostragem intencional com todas as amostras sendo cuidadores do Jardim de infância Cahaya Tazkia, Jardim de infância Yapita e Jardim de infância Aisyiah 52 Surabaya. Os dados foram analisados por meio de um teste de correlação de Spearman. Resultados: o benefício percebido e a autoeficácia tiveram correlação significativa com a experiência de acesso aos serviços de saúde bucal (p-valor ≤ 0,05), com coeficientes de correlação de 0,168 e 0,936, respectivamente. Enquanto isso, a suscetibilidade percebida, a severidade percebida e a barreira percebida não tiveram correlação com a experiência de acesso à saúde bucal. A maioria dos entrevistados do sexo masculino nesta pesquisa tinha um trabalho adequado para gerenciar a saúde bucal de seus filhos. A suscetibilidade percebida, a severidade percebida, o benefício percebido e a autoeficácia de cuidadores mulheres em idade produtiva foram bons, mas a barreira percebida apresentou uma média alta. Os entrevistados com essas características não tinham empregos fixos. Conclusão: mulheres cuidadoras na faixa etária de 21 a 40 anos acreditam que a manutenção da saúde bucal é benéfica e resultará em maior número de acessos aos serviços de saúde bucal de seus filhos. No entanto, cuidadoras que eram mulheres, mas que não tinham emprego fixo, apresentavam barreiras para o acesso à saúde bucal de seus filhos (AU)

The use of mucoadhesive oral patches containing epigallocatechin-3-gallate to treat periodontitis: an in vivo study.

Objectives: The application of topical drugs such as mucoadhesive oral patches (MOPs) do not irritate the mucosa and are able to increase the permeability of drugs to oral tissue. Epigallocatechin-3-gallate (EGCG) is an active ingredient that exhibits significant antibacterial and anti-inflammatory effects. The purpose of this study was to analyze the therapeutic potential of a mucoadhesive oral patch containing EGCG (MOP-EGCG) in a model of periodontitis and investigate its effects on the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kappa Β ligand (RANKL) and receptor activator of nuclear factor-κB (RANK). Methods: A model of periodontitis was induced in Rattus novergicus used Porphyromonas gingivalis by applying 0.03 ml of bacteria locally with 1 × 1010 colony-forming units (CFU) seven times at 2-day intervals in the central lower incisors. Periodontitis was then treated with MOP (control), a mucoadhesive oral patch containing doxycycline (MOP-doxy) or MOP-EGCG for 1 h/day for 21 days. On days 3, 5, 7, 14 and 21 after treatment, the central lower incisor was biopsied and analyzed by immunohistochemistry for RANK/RANKL and OPG expression in the gingiva tissue. Results: MOP-EGCG extract significantly reduced the expression of RANKL and increased the expression of OPG and RANK (p < 0.05) when compared to the MOP-doxy and MOP groups. Conclusion: MOP-EGCG extract reduced the expression of RANKL and increased the expression of OPG and RANK, thus suggesting that MOP-EGCG can inhibit the loss of alveolar bone in periodontitis.

Nano-emulsion of mangosteen rind extract in a mucoadhesive patch for periodontitis regenerative treatment: An in vivo study.

Objective: To investigate the therapeutic potential of nano-emulsion of mangosteen rind extract in a mucoadhesive gingival patch on periodontitis, and its effect on tumor necrosis factor alpha (TNF-α), receptor activator of nuclear factor kappa Β ligand (RANKL), and interleukin 10 (IL-10) expression. Methods: Sixty Wistar rats were divided into four groups: positive control group (mucoadhesive patch with doxycycline), negative control group (mucoadhesive patch), treatment group I (mucoadhesive patch with mangosteen rind extract), and treatment group II (mucoadhesive patch with nano-emulsion of mangosteen rind extract). An experimental model of Porphyromonas gingivalis-induced periodontitis was established in rats by treatment with 0.03 mL bacteria locally (1 × 1010 colony-forming units) seven times at 2-day intervals in the gingival sulcus of mandibular anterior teeth. Treatment was 1 h/day for 3 days. On days 3, 5, and 7, five rats from each group were killed. TNF-α, IL-10, and RANKL expression was determined by dissecting the lower jaw for immunohistochemistry. Results: The mucoadhesive patch with nano-emulsion mangosteen rind extract significantly decreased TNF-α and RANKL expression and increased IL-10 expression (p < 0.05) compared to the treatment I, positive and negative control groups. Conclusion: A mucoadhesive gingival patch with nano-emulsion of mangosteen rind extract has the potential to treat periodontitis by decreasing TNF-α, RANKL, and increasing IL-10 expression.

N-acetylcysteine versus chlorhexidine in treatment of aphthous ulcers: a preliminary clinical trial.

INTRODUCTION: This study sought to assess the efficacy of N-acetyl cysteine (NAC) in the treatment of recurrent aphthous stomatitis (RAS). METHODS: Fifty-eight patients, aged 28 ± 9.7 years, presented with clinically diagnosed RAS to two oral medicine centers. They were assigned randomly to a single application of either NAC (200 mg dissolved in water, n = 38) or 0.12% chlorhexidine digluconate (CHX, n = 20) mouthwashes for 30 seconds. Pain was measured using a Visual Analog Scale (VAS). The size of the ulcer was measured through its greatest dimension using a periodontal probe. These two measurements were taken pre-application (day 1) and 2nd, 4th, and 6th day post-application. Average time (in days) until complete healing was assessed. RESULTS: Of all participants, 33 (57%) were females; 34 (59%) married; 29 (50%) reported a family history of aphthae; and 51 (88%) were affected with minor RAS. There were greater improvement in pain from day 1 with NAC on the second day (-3.0 ± 2.0 versus -1.8 ± 1.9; p = .028) and on the fourth day (-5.0 ± 2.6 versus -3.4 ± 2.7; p = .041). The differences with regard to the change in ulcer size and average healing time were not significant between NAC and CHX. CONCLUSION: Single application of NAC results in a clinically significant reduction of RAS-associated pain within one day of application and is more effective than CHX.

Social Habits and Other Risk Factors that Cause Tooth Loss: An Associative Study Conducted in Taiz Governorate, Yemen.

AIMS: To investigate the relationship between tooth loss, age, gender, and its correlation with several local habits that affect oral health, especially the khat, and Shammah use. MATERIALS AND METHODS: The current study included 580 participants. They were divided into five age groups15-24, 25-34, 35-44, 45-54, and 55-64-years-old. Clinical and radiographic examinations were done for each subject. Reasons for teeth loss, age, gender, khat chewing, Shammah use, smoking, and use of oral hygiene aids were recorded. The data were statically analyzed using Chi-square tests of the statistical package for social sciences (SPSS) program. The p value < 0.05 were considered statistically significant. RESULTS: Caries was the most cause of tooth loss in the young age groups 15-34 years-old and was significantly more common compared to the older groups (p < 0.001). The periodontal diseases increased with age and progressively became the main cause of tooth loss of 100% in the 55-64 years-old group. Orthodontic reasons for tooth extractions were limited to the young age group 15-24 years old. Third molars were the highest extracted teeth in the maxillary arch, while the first molars were the highest in the mandibular arch. Anterior teeth were the lowest extracted teeth ranging from 9; 1.6% in maxillary teeth and 9-29; 1.6-5% in the mandibular teeth. CONCLUSION: This study highlights the importance of local habitual use of khat 52.9% and Shammah 8.1% besides smoking in tooth loss. Caries was the main cause of tooth loss in the young age groups, followed by the periodontal disease in the older age. Molars were frequently extracted followed by maxillary first premolars. CLINICAL SIGNIFICANCE: Limited number of epidemiological surveys were carried out in Taiz Governorate to investigate the reasons of tooth loss and its relationship with social habits like Khat chewing, Shammah use, smoking, using of Miswake and toothbrush. The results of this study will guide us to develop a preventive program which may minimize tooth loss and its adverse effects.

Effect of adjunctive use of systemic antioxidant therapy (N-acetylcysteine) on soluble receptor activator nuclear factor κB ligand levels in gingival crevicular fluid following surgical periodontal treatment for chronic periodontitis.

N-acetylcysteine (NAC) is an anti-oxidant drug that has been used as a mucolytic agent and a paracetamol antidote for many years. This study was designed to determine the efficacy of the adjunctive use of NAC for periodontal treatment. Thirty subjects with moderate-to-severe chronic periodontitis were randomized to surgery with NAC (600 mg; S-NAC), surgery only (S-nonNAC), and healthy control groups. Gingival crevicular fluid (GCF) samples were obtained from all patients and sRANKL levels were determined by enzyme-linked immunosorbent assay at baseline, and 1, 3, and 7 months post-surgery. Plaque and gingival indices, probing depths, and clinical attachment levels were recorded at the same time. There was a significant reduction in probing depth at 3 months in the S-NAC group when compared to the S-nonNAC group (P < 0.05). However, no statistically significant differences in plaque and gingival indices, probing depths, clinical attachment levels, and sRANKL levels in GCF were noted between the surgical treatment groups at the end of 7 months. Hence, the use of adjunctive NAC resulted in a significant reduction in probing depths in the S-NAC group when compared to the S-nonNAC group at 3 months, but no statistically significant differences in GCF sRANKL levels were observed in the sites that underwent surgical treatment with or without NAC at different time intervals.