ABSTRACT
Silver nanoparticles are opted to have various applications in different fields ranging from traditional medicines to culinary items. It is toxic and most effective against bacteria, fungi viruses, parasites, parasite carrying vectors such as mosquitoes and their larvae and other eukaryotic microorganisms at low concentration without any side effects and toxicity to humans. In view of these data, the present research has been investigated by synthesizing silver nanoparticles using 1mM silver nitrate and aqueous extract of Passiflora foetida. The variation of nanoparticles in size and shape concerning the concentration of extract prepared were analysed. The formation of silver nanoparticles was confirmed by colour changing from yellowish green to reddish-brown implicating the surface plasmon resonance. Further, it was concluded by obtaining an absorbance peak at 420 nm using UV-Visible spectrophotometer analysis. FTIR analysis was used to identify the capping ligands, which included alkanes, aromatic groups and nitro compounds. The average grain size of ~12 nm to 14 nm with crystalline phase was revealed by X-ray Diffraction studies. The SEM images depicted the surface morphology with agglomeration; TEM studies showed the shape of nanoparticles as spherical and hexagonal with sizes ranging from 40 nm to 100 nm and EDAX analysis confirmed the presence of elemental silver as the principal constituent. The characterized silver nanoparticles were then tested for synergistic antibacterial effects with tetracycline, and the results show that they are more active against E. coli and S. aureus, but moderately effective against B. cereus and K. pneumoniae . It also had a strong larval and pupal toxic effects on the dengue vector, Aedes aegypti with the highest mortality. As a result, silver nanoparticles could be a viable alternative for a variety of applications.
Subject(s)
Aedes , Insecticides , Metal Nanoparticles , Passiflora , Animals , Humans , Metal Nanoparticles/chemistry , Escherichia coli , Staphylococcus aureus , Mosquito Vectors , Plant Leaves/chemistry , Silver/pharmacology , Silver/analysis , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Plant Extracts/chemistry , Larva , Insecticides/pharmacologyABSTRACT
Background: In a pulpectomy, the eradication of microbes from the primary root canal is accomplished through biomechanical preparation, which could be carried out with either manual or rotary instruments. Aims: The objective of this clinical trial was to evaluate the efficiency of manual K-files, H-files, and Kedo-S Square rotary files in reducing microbial flora after canal preparation in primary molars. Materials and Methods: This randomized clinical trial consisted of 45 primary molars requiring pulpectomy. The teeth were randomly allocated to one of the three groups: Group I: Manual K-files, Group II: Manual H-files, and Group III: Kedo-S Square rotary files, based on the type of instrumentation. Pre-and Post-instrumentation sampling was performed using clean absorbent paper points and kept in a clean Eppendorf tube having thioglycolate broth as the transport medium. Culturing was performed on agar media from which both aerobic and anaerobic microbial counts were estimated. Collected data were statistically analyzed using one-way analysis of variance (ANOVA) and Wilcoxon signed-rank test. Following root canal preparation, 87-89% reduction of the aerobic and anaerobic microbial load was noted in group I, whereas it was an 89-92% reduction in group II and a 93-95% reduction in group III. Results: Biomechanical preparation with Kedo-S Square rotary file showed higher efficacy in microbial reduction compared to manual instrumentation. Conclusion: Manual and rotary files were equally effective in removing root canal microbes. Biomechanical preparation with a KedoS Square rotary file resulted in greater microbial efficacy. Hence In children, effective root canal cleaning in a short period of time is a major consideration.
Subject(s)
Root Canal Preparation , Tooth, Deciduous , Child , Humans , Pulpectomy , Root Canal Therapy , Dental Pulp CavityABSTRACT
In this research paper, an innovative electrochemical sensor was suggested for simultaneous voltammetric analysis of azithromycin (AZM) and hydroxychloroquine (HCQ) for the first time. The sensor based on hydrothermal synthesis of vanadium disulfide quantum dots (VS2 QDs) and insertion within 3D N, S graphene aerogel (3D N, S @ GNA) and carbon nanotubes nanaostructure as a new and widely group of carbon nanomaterials. The nanocomposites were characterized morphologically using different techniques. In addition, the nanomaterials were characterized electrochemically using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The proposed electrochemical sensor showed wide dynamic linear ranges of 0.28-30 × 10-8 M and 0.84-22.5 × 10-8 M for analysis of AZM and HCQ, respectively. The limits of detection (LODs) based on signal to noise (S/N) 3:1 were found to be 0.091 × 10-8 M and 0.277 × 10-8 M for AZM and HCQ, respectively. Briefly, the electrochemical sensor had good stability, selectivity, reproducibility and feasibility for simultaneous detection of AZM and HCQ in presence of different interfering species.
