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1.
Bratisl Lek Listy ; 121(12): 881-887, 2020.
Article in English | MEDLINE | ID: mdl-33300358

ABSTRACT

OBJECTIVE: There is frequently a relationship between nocturnal hypertension and non-dipping pattern and endothelial dysfunction. Studies conducted previously have indicated that adrenomedullin (AM) (a potent, long-lasting, vasodilatory peptide) is capable of regulating endothelial cell function. The aim of the current research is to investigate the association between absolute night-time blood pressure (BP) and circadian BP pat-tern with serum AM and high-sensitivity C-reactive protein (hsCRP) levels in cases in whom untreated arterial hypertension has been newly diagnosed. METHODS: Ambulatory BP monitoring was performed in 100 individuals with hypertension (50 dippers,50 non-dippers) and 50 healthy controls for 24 hours. Measurement and recording of AM and hsCRP serum levels were performed. RESULTS: A strong correlation between night-time BP levels and AM and hsCRP levels was determined(p<0.001). On the contrary, higher AM levels were determined in the non-dipper group compared to the dipper and normotensive groups (non-dipper group, 258±27 pg/mL; dipper group, 199±30 pg/mL; normotensive group, 150±11 pg/mL; p<0.001). The non-dipper group exhibited significantly higher hsCRP levels in comparison with the remaining two groups (p=0.017). An independent association was determined between AM (p=0.014) and hsCRP (p=0.032) and a non-dipping pattern in a multivariate logistic regression analysis. CONCLUSIONS: The nocturnal hypertensive and non-dipper groups exhibited increased AM levels. An independent association was identified between AM and hsCRP and a non-dipping pattern. It is implied that increased AM levels in individuals with non-dipper hypertension may be related to a longer exposure time to high BP. The mentioned findings indicate a potential future part of AM in identifying patients with hypertension that are at higher risk of target organ damage (Tab. 3, Fig. 4, Ref. 41).


Subject(s)
Adrenomedullin/blood , Circadian Rhythm , Essential Hypertension/diagnosis , Blood Pressure , Blood Pressure Monitoring, Ambulatory , C-Reactive Protein/analysis , Case-Control Studies , Humans
2.
Biotech Histochem ; 90(3): 223-30, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25747049

ABSTRACT

We determined the concentrations of goblet and immune cells in conjunctival imprints and tissues of canines with keratoconjunctivitis sicca (KCS) before and after cyclosporine A (CsA) treatment. Twelve dogs with bilateral KCS were assigned to three groups: untreated, treatment group 1, and treatment group 2. The treatment groups were treated topically with 2% ophthalmic CsA solution for 45 days; CsA treatment group 2 was followed for an additional 30 days after discontinuation of the drug. Schirmer tear test (STT) scores were recorded prior to CsA treatment and on alternate days throughout the experiment. CsA treatment improved the STT scores, restored conjunctival histology, increased goblet and epithelial cell numbers, and decreased numbers of inflammatory cells. Although the STT scores regressed slightly at day 30 after discontinuing the treatment, the scores were higher than the baseline values. Topical CsA treatment resolved clinical signs of KCS, improved STT scores and restored normal conjunctival histology.


Subject(s)
Conjunctiva/pathology , Cyclosporine/therapeutic use , Dog Diseases/drug therapy , Dog Diseases/pathology , Immunosuppressive Agents/therapeutic use , Keratoconjunctivitis Sicca/pathology , Keratoconjunctivitis Sicca/veterinary , Administration, Topical , Animals , Biopsy , Dogs , Female , Immunity, Mucosal , Immunosuppressive Agents/administration & dosage , Keratoconjunctivitis Sicca/drug therapy , Lymphocyte Subsets/pathology , Male , Mast Cells/pathology
3.
Transbound Emerg Dis ; 59(4): 303-10, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22085446

ABSTRACT

Forty pestivirus isolates sampled from cattle in Turkey between 2002 and 2007 were characterized according to 5' untranslated region (5'UTR) sequences and autoprotease (N(pro) ) gene sequences. The sampling of Bovine virus diarrhoea viruses (BVDVs) from 15 farms in five different regions indicated that BVDV 1-l (18/40, 45%) was the predominant genotype in Turkey; the samples also contained the genotypes 1-f (10/40, 25%), 1-b (7/40, 17.5%), 1-d (3/40, 7.5%), and 1-a (2/40, 5%), respectively.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/virology , Diarrhea Virus 1, Bovine Viral/genetics , Animals , Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Cattle , DNA, Viral/genetics , Genotype , Phylogeny , Turkey/epidemiology
4.
Transbound Emerg Dis ; 58(1): 31-6, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21040510

ABSTRACT

Forty cows between day 1 and day 21 post-calving were examined for the presence of postpartum metritis in a dairy herd that had recently experienced an increase in metritis and that had previously tested positive against bovine herpes virus 4 (BoHV-4) by various methods. Antibodies against BoHV-4 were detected in sera from 15 of 22 cows. For the virological study, uterine swab samples of 22 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation (VI), and immunofluorescence techniques. Twenty-two point seven per cent (5/22) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. All of these samples were also positive in VI and/or immune fluorescence assay (IF). Swab samples were also tested for bacteria. Empirical therapy with a broad spectrum antibiotic (oxytetracycline) was administrated, pending culture and antibiotic sensitivity result. All cows with puerperal metritis or clinical metritis (CM) were treated with intra-uterine (i.u.) administration of oxytetracycline and with intramuscular (i.m.) injections of dinoprost tromethamine (PGF(2)α) for three consecutive days. Concurrently, with the administration of oxytetracycline and PGF(2)α, cows with a rectal temperature >39.5°C received an additional treatment with oxytetracycline (i.m) for three consecutive days. According to the antibiotic test result, on day 3 after the last oxytetracycline and PGF(2)α administrations, all cows were treated with a combination of amoxicillin and clavulanic acid (i.u.) for three consecutive days. All cows with metritis and that were positive for BoHV-4 recovered clinically after the administration of antibiotic and PGF(2)α. In conclusion, postpartum metritis cases in cows infected BoHV-4 recovered clinically following early diagnosis and prolonged treatments with a combination of antibiotics and PGF(2)α.


Subject(s)
Bacteria/isolation & purification , Bacterial Infections/veterinary , Cattle Diseases/pathology , Dinoprost/analogs & derivatives , Herpesviridae Infections/veterinary , Herpesvirus 4, Bovine , Oxytetracycline/administration & dosage , Puerperal Infection/veterinary , Animals , Antibodies, Viral/blood , Antibodies, Viral/immunology , Bacteria/immunology , Bacterial Infections/pathology , Cattle , Cattle Diseases/microbiology , Cattle Diseases/virology , Dinoprost/administration & dosage , Dinoprost/therapeutic use , Female , Herpesviridae Infections/blood , Herpesviridae Infections/complications , Herpesviridae Infections/immunology , Oxytetracycline/therapeutic use , Postpartum Period/immunology , Puerperal Infection/microbiology , Puerperal Infection/pathology , Puerperal Infection/virology , Turkey , Uterine Diseases/complications , Uterine Diseases/pathology , Uterine Diseases/veterinary , Vagina/microbiology , Vagina/virology , beta-Lactams/administration & dosage , beta-Lactams/therapeutic use
5.
Transbound Emerg Dis ; 58(2): 179-85, 2011 Apr.
Article in English | MEDLINE | ID: mdl-26353053

ABSTRACT

We investigated bovine coronavirus (BCoV) as an etiological agent in cattle with clinical respiratory and digestive signs using 147 feces and 199 nasal swab samples. A total of 18 test samples (16 feces and 2 nasal swap samples) were detected positive by ELISA and/or RT-PCR targeting the BCoV N gene. The partial S1 gene regions of BCoVs (An-4 and An-11) detected in feces samples from two herd-mate dairy calves were compared. Virological and serological results indicated that BCoVs are widespread in Turkey and are likely etiological agents in diarrhea cases in calves.


Subject(s)
Cattle Diseases/epidemiology , Coronavirus Infections/veterinary , Coronavirus, Bovine/genetics , Spike Glycoprotein, Coronavirus/genetics , Animals , Cattle , Cattle Diseases/virology , Coronavirus Infections/epidemiology , Coronavirus Infections/virology , Coronavirus, Bovine/isolation & purification , Coronavirus, Bovine/metabolism , Enzyme-Linked Immunosorbent Assay/veterinary , Feces/virology , Molecular Sequence Data , Nose/virology , Phylogeny , Sequence Analysis, DNA/veterinary , Spike Glycoprotein, Coronavirus/metabolism , Turkey/epidemiology
6.
Transplant Proc ; 41(2): 756-63, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19328973

ABSTRACT

BACKGROUND: There is an emerging consensus on conversion from calcineurin inhibitor (CNI)-based regimens to proliferation signal inhibitor (PSI)-based protocols for the prevention of a progressive decline in graft function due to CNI toxicity. METHODS: Thirty-one primary renal transplant recipients within 17-48 years of age (mean, 32.2 +/- 1.6) were enrolled in this dual-center study. Eligible patients had a baseline (pre-engraftment) biopsy and completed at least 12 months of follow-up with deteriorating graft function indicative of chronic CNI toxicity with or without nonspecific interstitial fibrosis/tubular atrophy (IF/TA) on a biopsy specimen. A fast conversion protocol, being defined as a 50% initial reduction followed by complete withdrawal of CNI drug within 2 weeks of introducing rapamycin was performed in all patients. A sirolimus (SRL) loading dose was not prescribed; all subjects directly received maintenance (2-5 mg/d) doses of the drug. The primary endpoint of this study was assessement of renal function using cGFR and renal histology by protocol biopsy at 1 year after conversion. RESULTS: The mean follow-up after conversion was 21.6 months. The difference between cGFR before compared with cGFR after 12 months after conversion (40.8 +/- 2.36 mL/min vs 55.7 +/- 3.6 mL/min; P < .000) and at the last follow-up (40.8 +/- 2.36 mL/min vs 53.8 +/- 2.96 mL/min; P < .000) was significant. The mean IF/TA with glomerulosclerosis and chronic vasculopathy scores of biopsy specimens at baseline, during conversion, and at 12 months of the study were 2.25 +/- 0.3, 3.30 +/- 0.24, and 3.0 +/- 0.30, respectively. The change in scores was indicative of mild progression; however, the difference was not significant. IF/TA, glomerulosclerosis, and chronic vasculopathy scores improved in 8 (30%) subjects, remained unchanged in 11 (42%) and worsened in 7 (28%) after 1 year of SRL therapy. After conversion there was no patient or graft loss in this group. Moreover, SCr and GFR improved in 21 or 29 patients (72%), remained stable in 4 (14%), and decreased in 4 (14%) patients. The predictors of successful conversion in our study were GFR > or = 40.6 mL/min, SCr < or = 2.34 mg/dL, and histological allograft damage score < or =3. CONCLUSION: SRL-MPA/MMF-ST combination may be a good therapeutic strategy against chronic CNI toxicity, particularly for patients whose conversion biopsy specimens demonstrated mild IF/TA, glomerulosclerosis, and chronic vasculopathy scores (< or =3.1 +/- 0.3).


Subject(s)
Calcineurin/adverse effects , Immunosuppressive Agents/adverse effects , Kidney Transplantation/immunology , Sirolimus/therapeutic use , Adolescent , Adult , Biopsy , Blood Pressure , Cyclosporine/blood , Cyclosporine/therapeutic use , Follow-Up Studies , Glomerular Filtration Rate , Histocompatibility Testing , Humans , Immunosuppressive Agents/blood , Immunosuppressive Agents/therapeutic use , Kidney Transplantation/pathology , Kidney Transplantation/physiology , Middle Aged , Patient Selection , Prospective Studies , Sirolimus/blood , Tissue Donors/statistics & numerical data , Young Adult
7.
Vet Microbiol ; 135(3-4): 374-9, 2009 Mar 30.
Article in English | MEDLINE | ID: mdl-19019578

ABSTRACT

Blood samples from sheep and/or goats from eight small ruminant flocks in the Turkish provinces of Aydin and Burdur were tested for the presence of Pestiviruses using an antigen-capture ELISA. From clinically affected animals, pathological and immunohistochemical findings were recorded. Post mortem examination of a virus-positive lamb showing abnormal fleece and paralysis of the hind legs revealed nonsuppurative meningoencephalomyelitis with hypomyelinogenesis. By immunohistochemistry Pestivirus antigen was detected in all parts of the brain including cerebellum, cerebral hemispheres and midbrain. Two Pestivirus isolates from a sheep and a goat kid, respectively, were isolated from samples that were positive in the antigen-capture ELISA. Genetic typing using the 5'-NTR (288bp) and N(pro) (738bp) showed that both were Border disease virus (BDV) isolates. By phylogenetic analysis, they formed a cluster clearly separated from the known clusters BDV-1 to BDV-6 and might therefore represent a new subgroup (BDV-7?). This is the first report confirming the occurrence and partial characterisation of BDV infection in small ruminants in Turkey.


Subject(s)
Border Disease/epidemiology , Border disease virus/pathogenicity , Animals , Antibodies, Viral/immunology , Antigens, Viral/analysis , Antigens, Viral/blood , Border disease virus/classification , Border disease virus/genetics , Cerebrum/virology , Genotype , Geography , Goat Diseases/blood , Goat Diseases/epidemiology , Goat Diseases/virology , Goats/virology , Hindlimb/virology , Pestivirus/genetics , Pestivirus/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , Sheep , Sheep Diseases/blood , Sheep Diseases/epidemiology , Sheep Diseases/virology , Turkey/epidemiology
8.
Vet Res Commun ; 33(3): 241-9, 2009 Mar.
Article in English | MEDLINE | ID: mdl-18787968

ABSTRACT

In this study, a totally 164 materials (lung, spleen, lymph node, nasal and ocular swap, blood and samples from oral lesions) from sheep and lambs (n = 57) in the 34 flocks suspected the PPRV infection as clinically and macroscopic pathologic remarks, housed in the 4 different provinces in the Middle and Eastern Blacksea Region were used for RT-PCR and virus isolation. Additionally, serum samples randomly collected from 892 sheep were tested for the detection of PPRV seroprevalance in the same regions. The seroprevalance were estimated as 14,9% and 3,5-38,2% in the sampled animals and sampled province, respectively. While no virus isolated in Vero cell cultures, PPRV nucleic acid was detected in 26 of 164 materials by RT-PCR. According to the result of RT-PCR, the PPRV infection were diagnosed in 44,1% (15/34) and 31,5% (18/57) of the flocks and sampled animals, respectively. Diagnostic value of necropsy materials such as lymph node, spleen, lung and of clinical samples such as nasal swap and conjunctival swap were determined more valuable diagnostic materials in the diagnosis of PPRV infection by RT-PCR. Data showed that PPRV infection was widespread in the Middle and East Blacksea Region and that the prevalence of the infection in the region varies in accordance with the factors such as geographical conditions (climate, etc.) and the method of breeding. Additionally, it is determined that RT-PCR is sensitive and reliable method in the diagnosis of PPRV infection.


Subject(s)
Morbillivirus Infections/veterinary , Morbillivirus/isolation & purification , Sheep Diseases/virology , Animals , Enzyme-Linked Immunosorbent Assay/veterinary , Morbillivirus/genetics , Morbillivirus Infections/diagnosis , Morbillivirus Infections/epidemiology , Morbillivirus Infections/virology , RNA, Viral/chemistry , RNA, Viral/genetics , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Seroepidemiologic Studies , Sheep , Sheep Diseases/diagnosis , Sheep Diseases/epidemiology , Turkey/epidemiology
9.
Arch Virol ; 152(6): 1175-85, 2007.
Article in English | MEDLINE | ID: mdl-17294092

ABSTRACT

Two genotypes of foot-and-mouth disease virus serotype A were identified as the cause of disease outbreaks in Turkey during 1996-2004, while serotype O strains, identified during the same period, seem to represent an evolutionary continuum, and Asia1 strains were only rarely identified. The data presented are concordant with the conclusion that serotype A strains are repeatedly introduced to Turkey from the east and circulate only transiently in farming communities, while type O strains persist and re-emerge from endemic areas of Turkey. The co-circulation of strains belonging to two A genotypes for 6 years, as observed in the present study, is a remarkable difference compared to previous decades in which only one A genotype was transiently circulating, successively being replaced by others. This co-circulation was observed in spite of enforcement countrywide of biannual vaccination of more than 50% of the cattle during the same period. Mean r(1) values of 0.70 +/- 0.19 and 0.39 +/- 0.04 found for A96 and A99 isolates, respectively, compared to the A96 vaccine component reveal antigenic differences but also imply that the vaccine in use in Turkey should provide protection against both genotypes. It is suggested that further studies to reveal the nature of the difference in epidemiological dynamics of type A and type O strains might lead to an understanding of the measures required to control foot-and-mouth disease in islands of persistent circulation.


Subject(s)
Foot-and-Mouth Disease Virus/isolation & purification , Animals , Base Sequence , Capsid Proteins/genetics , Cattle , Cattle Diseases/epidemiology , Cattle Diseases/virology , DNA Primers/genetics , DNA, Viral/genetics , Foot-and-Mouth Disease/epidemiology , Foot-and-Mouth Disease/virology , Foot-and-Mouth Disease Virus/classification , Foot-and-Mouth Disease Virus/genetics , Genotype , Phylogeny , Serotyping , Time Factors , Turkey/epidemiology
10.
Vet Res Commun ; 30(8): 965-71, 2006 Nov.
Article in English | MEDLINE | ID: mdl-17139549

ABSTRACT

Poxvirus epidemics occur almost every year and cause significant economic losses for small-scale animal producers in Turkey. In this study, the causative agent of the most recent epidemic in Central Anatolia was detected in clinical samples using electron microscopy (EM) and amplified using an in house polymerase chain reaction procedure for the first time. Additionally, the aetiological agent was isolated from a sheep and identified using EM and PCR.


Subject(s)
Capripoxvirus/classification , Capripoxvirus/isolation & purification , Disease Outbreaks/veterinary , Poxviridae Infections/veterinary , Sheep Diseases/virology , Animals , Capripoxvirus/genetics , Poxviridae Infections/epidemiology , Poxviridae Infections/virology , Sheep Diseases/epidemiology , Turkey/epidemiology
11.
Dtsch Tierarztl Wochenschr ; 112(7): 271-4, 2005 Jul.
Article in English | MEDLINE | ID: mdl-16124702

ABSTRACT

Serum samples of 15,909 cattle from 31 dairy herds located in various regions of Turkey were tested for the presence of antibodies against bovine leucosis virus (BLV) using Agar Gel Immuno-diffusion technique (AGID). 48.3% (15/31) of the herds had seropositive animals and positivity rates were detected from 0.5-34.4% in these herds. In an EBL control/eradication programme all seropositive animals were culled in the infected herds. Thereafter, a total of 74,347 sera were tested for the presence of BLV specific antibodies. The serological results and detail of EBL control/eradication programme were shown in this paper.


Subject(s)
Antibodies, Viral/blood , Enzootic Bovine Leukosis/prevention & control , Leukemia Virus, Bovine/immunology , Age Factors , Animals , Cattle , Dairying/methods , Enzootic Bovine Leukosis/epidemiology , Female , Immunodiffusion/veterinary , Seroepidemiologic Studies , Turkey/epidemiology
12.
Vet J ; 164(3): 280-7, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12505405

ABSTRACT

The effect of therapeutic ultrasound (US) on the growth plates of growing bone was determined through fluorescence labelling and histopathological evaluation in one-month-old rabbits. Mean growth rates and mean heights of the growth plates of the US exposed radius vs. control radius, and US exposed ulna vs. control ulna showed no statistically significant differences at all intensities and time intervals. Mean growth rates and heights of the growth plates of the US exposed radius for 0.2 W/cm(2) (SATA) US vs. 0.5 W/cm(2) US showed the same type of pattern compared to those of the control radius for 0.2 W/cm(2)vs. 0.5 W/cm(2) at days 10, 15 and 20. Similar results were also found for the ulna. Effects of 0.5 W/cm(2) US on the mean growth rates and mean heights of the growth plates were superior to those of 0.2 W/cm(2) US at day 10. The results at day 10 were reversed for the same comparisons at days 15 and 20. No cavitation effect of US was observed on growth plate cells. There was no evidence of partial or complete premature closure of the growth plates or of angular deformity of the carpal region.


Subject(s)
Bone Development , Forelimb/diagnostic imaging , Forelimb/growth & development , Growth Plate/diagnostic imaging , Growth Plate/growth & development , Ultrasonography/adverse effects , Animals , Contraindications , Female , Forelimb/pathology , Growth Plate/pathology , Male , Rabbits , Radius/diagnostic imaging , Radius/growth & development , Radius/pathology , Ulna/diagnostic imaging , Ulna/growth & development , Ulna/pathology
13.
Dtsch Tierarztl Wochenschr ; 107(5): 193-5, 2000 May.
Article in English | MEDLINE | ID: mdl-10887671

ABSTRACT

Nasal cells extracted from nasal swabs obtained from 95 cattle with signs of respiratory disease, out of eleven different herds, were tested for BHV-1, PI-3 virus, BRSV and BVDV using direct immunofluorescence technique. Viral antigen positive samples were detected in seven out of eleven herds examined. Of the 95 individual diseased cattle, 19 were found positive for at least one viral antigen. It was found that especially BHV-1 and PI-3 virus are important causative agents in cattle respiratory disease, both or in combination with other pathogenic agents. Multiple infection in virologically positive herds were observed in six (9.8%) of 61 animals tested. The findings reveal that single or multiple infections of selected viruses may be present in an important range in cattle and that direct immunofluorescence technique as a rapid method, based on the detection of viral antigen in nasal swab samples, is useful to establish the viral aetiology of acute bovine respiratory disease caused by these viruses, particularly in the diagnosis of mixed viral infections.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/diagnosis , Cattle Diseases/virology , Herpesviridae Infections/veterinary , Herpesvirus 1, Bovine/isolation & purification , Respiratory Tract Infections/veterinary , Respirovirus Infections/veterinary , Respirovirus/isolation & purification , Animals , Cattle , Cattle Diseases/diagnosis , Diarrhea Viruses, Bovine Viral/isolation & purification , Fluorescent Antibody Technique, Direct , Herpesviridae Infections/diagnosis , Respiratory Tract Infections/diagnosis , Respiratory Tract Infections/virology , Respirovirus Infections/diagnosis
14.
Dtsch Tierarztl Wochenschr ; 105(7): 276-8, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9697353

ABSTRACT

Serum/colostrum pairs were collected from 245 ewes in 6 sheep herds which had been determined previously to be infected with MV virus and were tested against maedi-visna infection using AGID test. Positive rates were detected as 3.8-41.2% in tested flocks. Serum and colostrum samples obtained from 53 sheep were positive for MV virus specific antibodies by AGID test. 16 colostrum samples were negative although serum samples obtained from the same animals were found to be positive for MV antibodies. Of the 245 sera and colostrum pairs tested, there was total agreement of results (+ or -) in 229 and disagreement in the results with the other 16 serum/colostrum pairs. Of the latter, all serum samples were positive and all colostrum samples were negative for MV antibodies. This study compared colostrum and serum samples for the determination of MV antibodies using AGID test under field conditions on naturally infected animals and on healthy animals. The results show that colostrum antibodies can be detected using AGID test and that colostrum is a reliable material to determine anti-MV virus antibodies. The procedure can be used for herd diagnosis.


Subject(s)
Antibodies, Viral/analysis , Colostrum/immunology , Visna/diagnosis , Animals , Antibodies, Viral/blood , Colostrum/virology , Female , Immunodiffusion/methods , Sheep , Visna/blood , Visna/immunology , Visna-maedi virus
15.
Dtsch Tierarztl Wochenschr ; 101(10): 390-1, 1994 Oct.
Article in English | MEDLINE | ID: mdl-7851298

ABSTRACT

In this study, a serological survey in goat herds for antibodies against Caprine Arthritis Encephalitis Virus (CAEV) in Turkey is described. 808 serum samples were collected from three state farms and seven private flocks in different regions of Turkey. The serologic examination was performed by Agar Gel Immunodiffusion (AGID) technique. 16 (1.9%) out of 808 sera deriving from two state farms (n = 14) and one private herd (n = 2) were found to be serologically positive. The findings are discussed with regard to the relatively low prevalence of CAEV in Turkey, age and race dependency and the kind of origin of the serologically screened animals.


Subject(s)
Antibodies, Viral/blood , Arthritis-Encephalitis Virus, Caprine/immunology , Goat Diseases/epidemiology , Lentivirus Infections/veterinary , Animals , Goats , Immunodiffusion/veterinary , Lentivirus Infections/epidemiology , Prevalence , Seroepidemiologic Studies , Turkey/epidemiology
16.
Dtsch Tierarztl Wochenschr ; 100(3): 107-9, 1993 Mar.
Article in English | MEDLINE | ID: mdl-8386080

ABSTRACT

The role of Bovine Viral Diarrhoea (BVD) virus in calves born with congenital brain abnormalities in western, southern and southeastern parts of Anatolia was investigated. 639 blood serum samples collected from cows which had aborted or given birth to calves with congenital abnormalities, from their offspring and from clinically healthy animals out of the above mentioned regions were tested for the presence of BVD antibodies. Additionally BVD virus isolation was performed with material obtained from cows which had aborted or given birth to calves with congenital abnormalities and from their offspring. Out of 639 blood sera 203 (31.7%) were found serologically positive, and from samples of 30 animals tested by direct immunofluorescence technique 13 (7 cows and 6 calves) BVD virus carriers could be demonstrated. The findings reveal that BVD virus infections might be a cause of malformations found in these calves born with congenital abnormalities of the central nervous system.


Subject(s)
Antibodies, Viral/blood , Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Brain Diseases/microbiology , Diarrhea Viruses, Bovine Viral/immunology , Pregnancy Complications, Infectious/veterinary , Animals , Bovine Virus Diarrhea-Mucosal Disease/complications , Brain Diseases/congenital , Cattle , Female , Incidence , Pregnancy , Pregnancy Complications, Infectious/epidemiology , Turkey/epidemiology
17.
Dtsch Tierarztl Wochenschr ; 98(6): 226-8, 1991 Jun.
Article in English | MEDLINE | ID: mdl-1653695

ABSTRACT

The prevalence of enzootic bovine leukosis (EBL) in Turkey was investigated by serological screenings using the agargel immunodiffusion test (AGID). A total of 4,047 blood samples obtained from 2,780 cattle between one and 14 years of age and taken in 6-8 month-intervals in three dairy farms were tested for the presence of EBL-specific antibodies. A high prevalence of EBL-sero-positive animals was found amongst cattle in two of the farms. Furthermore, recommendations based upon the regulation implemented by an expert group of the European Commission and considering the local dairy management conditions were presented.


Subject(s)
Antibodies, Viral/blood , Leukemia Virus, Bovine/immunology , Leukemia/veterinary , Animals , Cattle , Leukemia/epidemiology , Prevalence , Turkey/epidemiology
18.
Dtsch Tierarztl Wochenschr ; 97(2): 88-9, 1990 Feb.
Article in English | MEDLINE | ID: mdl-2311535

ABSTRACT

In this study, a survey of cattle for neutralizing antibodies to respiratory syncytial virus (RSV) in Turkey is presented. 490 serum samples were collected from several state farms and some private flocks in different regions of Turkey. The serologic examination was done by the microtiter serum-neutralization technique. In this report, a positive serum antibody titer at dilutions of 1:2 was demonstrated in 226 (46.12%) of the 490 sera tested.


Subject(s)
Antibodies, Viral/analysis , Cattle Diseases/epidemiology , Respiratory Syncytial Viruses/immunology , Respirovirus Infections/veterinary , Animals , Cattle , Neutralization Tests , Prevalence , Respirovirus Infections/epidemiology , Turkey/epidemiology
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