ABSTRACT
Carbon monoxide (CO) is the most common cause of fatal poisoning all over the world. At the cellular level, a combination of tissue hypoxia and direct cellular damage underlie the pathophysiology of CO toxicity. The purpose of this study was to determine the effect of CO treatment on oxidative stress parameters in mitochondria isolated from male and female rat brains. Mitochondria prepared from frontal cortex, hippocampus and corpus striatum were treated with 0.1% CO at 37 degrees C for 30 minutes; control samples were not exposed to CO. Cytochrome c oxidase activity (COX), lipid peroxidation (thiobarbituric acid reactive species = TBARS), protein oxidation (protein carbonyls) and glutathione (GSH) levels were measured in CO treated and control samples. Our results confirmed previous studies reporting the inhibition of cytochrome c oxidase activity by CO in rat brain. Additionally, protein carbonyl levels in the hippocampus and striatum significantly increased after CO treatment in male rats. While CO treatment caused a significant decrease in GSH levels in the cortex and striatum in male rats, reduced GSH levels were observed in the cortex and hippocampus in female rats following CO exposure. Taken together, our data suggest a role for mitochondrial oxidative stress in CO toxicity at the cellular level during CO poisoning.
Subject(s)
Brain/drug effects , Carbon Monoxide/toxicity , Mitochondria/drug effects , Oxidative Stress/drug effects , Animals , Brain/enzymology , Brain/metabolism , Cerebral Cortex/drug effects , Cerebral Cortex/metabolism , Corpus Striatum/drug effects , Corpus Striatum/metabolism , Electron Transport Complex IV/metabolism , Female , Glutathione/metabolism , Hippocampus/drug effects , Hippocampus/metabolism , In Vitro Techniques , Lipid Peroxidation/drug effects , Male , Mitochondria/enzymology , Mitochondria/metabolism , Protein Carbonylation/drug effects , Rats , Rats, Sprague-DawleyABSTRACT
Intraurethral instillation was used in place of intracavernosal injection of vasoactive agents for the colour Doppler investigation of patients with erectile dysfunction. A total of 19 patients were enrolled in this study. Colour penile Doppler ultrasonography was done with intracavernosal injection of papaverine-HCl 60 mg and intraurethral instillation of prostaglandin-E2 0.5 mg. Arterial diameter, peak systolic velocity and end diastolic velocity were measured before and after papaverine injection and prostaglandin-E2 instillation. Colour penile Doppler parameters increased statistically significantly after papaverine injection and prostaglandin-E2 instillation (p < 0.01). But we did not observe differences between the results after papaverine-HCl and prostaglandin-E2. We believe that prostaglandin-E2 may be used with colour Doppler ultrasonography in the evaluation of patients with erectile dysfunction.
Subject(s)
Dinoprostone , Erectile Dysfunction/diagnostic imaging , Oxytocics , Papaverine , Penis/diagnostic imaging , Ultrasonography, Doppler, Color , Vasodilator Agents , Adult , Erectile Dysfunction/physiopathology , Hemodynamics , Humans , Instillation, Drug , Male , Middle AgedABSTRACT
The CT features of cerebral hydatid cysts are divided into two groups. In the unilocular cases, the cyst is large, spherical in shape with sharply defined borders. One side of the cyst wall is very close to the calvarium. The cyst may calcify and may have septae inside. There is no enhancement with contrast media and also no edema surrounding the cyst. In the second group there are multiple and small multilocular cysts. These cysts are in ovoid or polygonal forms like a bunch of grapes, with perifocal edema. After contrast media administration, there is enhancement in the cyst wall. In one case there were numerous cysts in both hemispheres.
