Complementary and Alternative Medicine Use Among Patients with Dry Eye Syndrome in Saudi Arabia: A Survey Study.

Introduction: Complementary and alternative medicine (CAM) includes all healthcare practices that are not part of conventional medicine. One of the most common eye disorders for visiting an ophthalmology clinic is dry eye disease (DED), and due to the increasing number of CAM used for eye conditions, 1 in 5 patients has been reported in previous studies to use CAM for eye treatment. This study aimed to estimate the prevalence of CAM use among patients with (DED). Methods: A web-based survey was used to collect the data, the first part of the questionnaire was about demographic data, and the second part included a validated Arabic version of the ocular surface disease index (OSDI). The third part was about practices that alleviate DED other than conventional medicine. Results: The total sample was 282, 61 were males, and 221 were females. Most participants (48.5%) were between 25 and 44 years old, 90% were Saudi, and only 10% were non-Saudi. Of the participant, 70% reported having attained a university or higher education level. The prevalence of the use of CAM among patients with dry eye was around 85%. The most frequently reported type of CAM used among the participants was faith healing (Ruqyah) (41%). This was followed by warm compressors (37%). There was no significant correlation between the severity of OSDI and using CAM, as the p-value was 0.909. Conclusion: In conclusion, our study shows that CAM is a popular choice among patients with dry eye syndrome. Faith healing and warm compressors are the most used types of CAM. However, the lack of correlation between OSDI severity and CAM use indicates that patients may use CAM for reasons other than symptom severity. Further research is needed to explore the reasons behind CAM use and its effectiveness in managing dry eye syndrome.

SARS-CoV-2 RBD protein enhances the oncolytic activity of the vesicular stomatitis virus.

Despite recent advances in the research on oncolytic viruses (OVs), a better understanding of how to enhance their replication is key to improving their therapeutic index. Understanding viral replication is important to improve treatment outcomes based on enhanced viral spreading within the tumor milieu. The VSV-Δ51 oncolytic virus has been widely used as an anticancer agent with a high selectivity profile. In this study, we examined the role of the SARS-CoV-2 spike protein receptor-binding domain (RBD) in enhancing VSV-Δ51 viral production and oncolytic activity. To test this hypothesis, we first generated a novel VSV-Δ51 mutant that encoded the SARS-COV-2 RBD and compared viral spreading and viral yield between VSV-Δ51-RBD and VSV-Δ51 in vitro. Using the viral plaque assay, we demonstrated that the presence of the SARS-CoV-2 RBD in the VSV-Δ51 genome is associated with a significantly larger viral plaque surface area and significantly higher virus titers. Subsequently, using an ATP release-based assay, we demonstrated that the SARS-CoV-2 RBD could enhance VSV-Δ51 oncolytic activity in vitro. This observation was further supported using the B16F10 tumor model. These findings highlighted a novel use of the SARS-CoV-2 RBD as an anticancer agent.

Awareness About Keratoconus and Its Relation With Eye Rubbing: A Cross-Sectional Study in Medina.

BACKGROUND: Keratoconus is a non-inflammatory, bilateral, asymmetrical progressive disorder characterized by ectasia, thinning, and increased curvature of the cornea, as well as loss of visual acuity. Eye rubbing is considered the most common risk factor for keratoconus. OBJECTIVES: This study aims to assess the awareness of the population in Medina about keratoconus and its relation to eye rubbing. METHODS: This is a cross-sectional study involving 767 participants via an online pre-designed questionnaire from November 2021 to January 2022, in Medina, Saudi Arabia.  Results: Among the study participants, 94.1% have a lack of awareness about keratoconus and its relation to eye rubbing. Participants who have a visual disturbance and positive family history of keratoconus were found to have good awareness levels. Those who heard about keratoconus represent 39.8% of the participants, and relatives with keratoconus were the most common source of their information. An allergic reaction was reported by 34.9% of the participants, and 7.7% have a family history of keratoconus. Only 27.8% believe in the relationship between keratoconus and allergy, and 61.9% have no idea about the treatment. For eye rubbing, 28.9% of participants believe it can lead to keratoconus; also, 80.4% reported rubbing their eyes, and itching was the most common cause of their behavior. CONCLUSION: The majority of the participants have a lack of awareness about keratoconus and its relation to eye rubbing. Health education programs for the population should be conducted to enhance public awareness about keratoconus.