Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 10 de 10
Filter
Add more filters










Publication year range
1.
Biomater Sci ; 8(14): 3896-3906, 2020 Jul 21.
Article in English | MEDLINE | ID: mdl-32539053

ABSTRACT

In order to infuse hemoglobin into the vasculature as an oxygen therapeutic or blood substitute, it is necessary to increase the size of the molecule to enhance vascular retention. This aim can be achieved by PEGylation. However, using non-specific conjugation methods creates heterogenous mixtures and alters protein function. Site-specific PEGylation at the naturally reactive thiol on human hemoglobin (ßCys93) alters hemoglobin oxygen binding affinity and increases its autooxidation rate. In order to avoid this issue, new reactive thiol residues were therefore engineered at sites distant to the heme group and the α/ß dimer/dimer interface. The two mutants were ßCys93Ala/αAla19Cys and ßCys93Ala/ßAla13Cys. Gel electrophoresis, size exclusion chromatography and mass spectrometry revealed efficient PEGylation at both αAla19Cys and ßAla13Cys, with over 80% of the thiols PEGylated in the case of αAla19Cys. For both mutants there was no significant effect on the oxygen affinity or the cooperativity of oxygen binding. PEGylation at αAla19Cys had the additional benefit of decreasing the rates of autoxidation and heme release, properties that have been considered contributory factors to the adverse clinical side effects exhibited by previous hemoglobin based oxygen carriers. PEGylation at αAla19Cys may therefore be a useful component of future clinical products.


Subject(s)
Hemoglobins , Polyethylene Glycols , Chromatography, Gel , Heme , Humans , Oxygen
2.
Biosci Rep ; 38(4)2018 08 31.
Article in English | MEDLINE | ID: mdl-29802155

ABSTRACT

Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been engineered to replace or augment the oxygen carrying capacity of erythrocytes. However, clinical results have generally been disappointing, in part due to the intrinsic oxidative toxicity of Hb. The most common HBOC starting material is adult human or bovine Hb. However, it has been suggested that fetal Hb may offer advantages due to decreased oxidative reactivity. Large-scale manufacturing of HBOC will likely and ultimately require recombinant sources of human proteins. We, therefore, directly compared the functional properties and oxidative reactivity of recombinant fetal (rHbF) and recombinant adult (rHbA) Hb. rHbA and rHbF produced similar yields of purified functional protein. No differences were seen in the two proteins in: autoxidation rate; the rate of hydrogen peroxide reaction; NO scavenging dioxygenase activity; and the NO producing nitrite reductase activity. The rHbF protein was: less damaged by low levels of hydrogen peroxide; less damaging when added to human umbilical vein endothelial cells (HUVEC) in the ferric form; and had a slower rate of intrinsic heme loss. The rHbA protein was: more readily reducible by plasma antioxidants such as ascorbate in both the reactive ferryl and ferric states; less readily damaged by lipid peroxides; and less damaging to phosphatidylcholine liposomes. In conclusion in terms of oxidative reactivity, there are advantages and disadvantages to the use of rHbA or rHbF as the basis for an effective HBOC.


Subject(s)
Blood Substitutes/metabolism , Fetal Hemoglobin/metabolism , Hemoglobins/metabolism , Adult , Animals , Human Umbilical Vein Endothelial Cells , Humans , Nitric Oxide/metabolism , Oxidation-Reduction , Oxidative Stress , Recombinant Proteins/metabolism
3.
J Biol Chem ; 290(12): 7426-35, 2015 Mar 20.
Article in English | MEDLINE | ID: mdl-25616660

ABSTRACT

Aggregation of α-synuclein (α-syn) into toxic fibrils is a pathogenic hallmark of Parkinson disease (PD). Studies have focused largely on residues 71-82, yet most early-onset mutations are located between residues 46 and 53. A semirationally designed 209,952-member library based entirely on this region was constructed, containing all wild-type residues and changes associated with early-onset PD. Intracellular cell survival screening and growth competition isolated a 10-residue peptide antagonist that potently inhibits α-syn aggregation and associated toxicity at a 1:1 stoichiometry. This was verified using continuous growth measurements and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity studies. Atomic force microscopy and circular dichroism on the same samples showed a random-coil structure and no oligomers. A new region of α-syn for inhibitor targeting has been highlighted, together with the approach of using a semirational design and intracellular screening. The peptides can then be used as candidates for modification in drugs capable of slowing or even preventing the onset of PD.


Subject(s)
Peptide Library , Peptides/pharmacology , alpha-Synuclein/antagonists & inhibitors , Alzheimer Disease/metabolism , Amino Acid Sequence , Base Sequence , Circular Dichroism , DNA Primers , Lewy Bodies/metabolism , Microscopy, Atomic Force , Parkinson Disease/metabolism , Peptides/chemistry , Polymerase Chain Reaction , alpha-Synuclein/metabolism
4.
J Cell Sci ; 121(Pt 6): 796-803, 2008 Mar 15.
Article in English | MEDLINE | ID: mdl-18285448

ABSTRACT

Phosphatidylinositol transfer proteins (PITPs) mediate the transfer of phosphatidylinositol (PtdIns) or phosphatidylcholine (PtdCho) between two membrane compartments, thereby regulating the interface between signalling, phosphoinositide (PI) metabolism and membrane traffic. Here, we show that PITPalpha is enriched in specific areas of the postnatal and adult brain, including the hippocampus and cerebellum. Overexpression of PITPalpha, but not PITPbeta or a PITPalpha mutant deficient in binding PtdIns, enhances laminin-dependent extension of axonal processes in hippocampal neurons, whereas knockdown of PITPalpha protein by siRNA suppresses laminin and BDNF-induced axonal growth. PITPalpha-mediated axonal outgrowth is sensitive to phosphoinositide 3-kinase (PI3K) inhibition and shows dependency on the Akt/GSK-3/CRMP-2 pathway. We conclude that PITPalpha controls the polarized extension of axonal processes through the provision of PtdIns for localized PI3K-dependent signalling.


Subject(s)
Axons/ultrastructure , Hippocampus/embryology , Neurons/cytology , Neurons/enzymology , Phosphatidylinositol 3-Kinases/metabolism , Phospholipid Transfer Proteins/metabolism , Animals , Axons/enzymology , Brain/growth & development , Brain Chemistry , Cells, Cultured , Hippocampus/cytology , Hippocampus/growth & development , Neurons/chemistry , Phospholipid Transfer Proteins/analysis , Phospholipid Transfer Proteins/antagonists & inhibitors , RNA Interference , Rats , Signal Transduction
5.
Biochem J ; 398(3): 411-21, 2006 Sep 15.
Article in English | MEDLINE | ID: mdl-16780419

ABSTRACT

Mammalian PITPbeta (phosphatidylinositol transfer protein beta) is a 272-amino-acid polypeptide capable of transferring PtdIns, PtdCho and SM (sphingomyelin) between membrane bilayers. It has been reported that Ser262 present in the C-terminus of PITPbeta is constitutively phosphorylated and determines Golgi localization. We provide evidence for the expression of an sp (splice) variant of PITPbeta (PITPbeta-sp2) where the C-terminal 15 amino acids of PITPbeta-sp1 are replaced by an alternative C-terminus of 16 amino acids. PITPbeta-sp1 is the product of the first 11 exons, whereas PITPbeta-sp2 is a product of the first 10 exons followed by the twelfth exon--exon 11 being 'skipped'. Both splice variants are capable of PtdIns and PtdCho transfer, with PITPbeta-sp2 being unable to transport SM. PITPbeta is ubiquitously expressed, with the highest amounts of PITPbeta found in HL60 cells and in rat liver; HL60 cells express only PITPbeta-sp1, whereas rat liver expresses both sp variants in similar amounts. In both cell types, PITPbeta-sp1 is constitutively phosphorylated and both the PtdIns and PtdCho forms of PITPbeta-sp1 are present. In contrast, PITPbeta-sp2 lacks the constitutively phosphorylated Ser262 (replaced with glutamine). Nonetheless, both PITPbeta variants localize to the Golgi and, moreover, dephosphorylation of Ser262 of PITPbeta-sp1 does not affect its Golgi localization. The presence of PITPbeta sp variants adds an extra level of proteome complexity and, in rat liver, the single gene for PITPbeta gives rise to seven distinct protein species that can be resolved on the basis of their charge differences.


Subject(s)
Alternative Splicing/physiology , Golgi Apparatus/metabolism , Phospholipid Transfer Proteins/metabolism , Amino Acid Sequence , Amino Acid Substitution , Animals , COS Cells , Chlorocebus aethiops , Cytosol , Gene Expression Regulation , HL-60 Cells , Humans , Liver/cytology , Molecular Sequence Data , Phosphorylation , Protein Binding , Protein Transport , Rats
6.
J Biol Chem ; 279(45): 47159-71, 2004 Nov 05.
Article in English | MEDLINE | ID: mdl-15322105

ABSTRACT

Phosphatidylinositol transfer protein alpha (PITPalpha) participates in the supply of phosphatidylinositol (PI) required for many cellular events including phospholipase C (PLC) beta and gamma signaling by G-protein-coupled receptors and receptor-tyrosine kinases, respectively. Protein kinase C has been known to modulate PLC signaling by G-protein-coupled receptors and receptor-tyrosine kinases, although the molecular target has not been identified in most instances. In each case phorbol myristate acetate pretreatment of HL60, HeLa, and COS-7 cells abrogated PLC stimulation by the agonists formyl-Met-Leu-Phe, ATP, and epidermal growth factor, respectively. Here we show that phosphorylation of PITPalpha at Ser166 resulted in inhibition of receptor-stimulated PLC activity. Ser166 is localized in a small pocket between the 165-172 loop and the rest of the protein and was not solvent-accessible in either the PI- or phosphatidylcholine-loaded structures of PITPalpha. To allow phosphorylation at Ser166, a distinct structural form is postulated, and mutation of Thr59 to alanine shifted the equilibrium to this form, which could be resolved on native PAGE. The elution profile observed by size exclusion chromatography of phosphorylated PITPalpha from rat brain or in vitro phosphorylated PITPalpha demonstrated that phosphorylated PITPalpha is structurally distinct from the non-phosphorylated form. Phosphorylated PITPalpha was unable to deliver its PI cargo, although it could deliver phosphatidylcholine. We conclude that the PITPalpha structure has to relax to allow access to the Ser166 site, and this may occur at the membrane surface where PI delivery is required for receptor-mediated PLC signaling.


Subject(s)
Phospholipid Transfer Proteins/chemistry , Protein Kinase C/metabolism , Serine/chemistry , Animals , Binding Sites , Brain/embryology , Brain/metabolism , COS Cells , Cell Membrane/metabolism , Chromatography , Chromatography, High Pressure Liquid , Cytosol/metabolism , Dose-Response Relationship, Drug , Electrophoresis, Polyacrylamide Gel , Green Fluorescent Proteins/metabolism , HL-60 Cells , HeLa Cells , Humans , Isoelectric Focusing , Lipid Metabolism , Microscopy, Confocal , Models, Molecular , Mutagenesis, Site-Directed , Mutation , Peptides/chemistry , Phosphorylation , Protein Conformation , Protein Structure, Tertiary , Rats , Recombinant Proteins/chemistry , Signal Transduction , Tetradecanoylphorbol Acetate , Threonine/metabolism , Time Factors , Transfection , Type C Phospholipases/chemistry , Type C Phospholipases/metabolism
7.
Curr Biol ; 13(1): 78-84, 2003 Jan 08.
Article in English | MEDLINE | ID: mdl-12526750

ABSTRACT

Phosphatidylinositol transfer proteins (PITP) function in signal transduction and in membrane traffic. Studies aimed at elucidating the mechanism of action of PITP have yielded a singular theme; the activity of PITP stems from its ability to transfer phosphatidylinositol (PI) from its site of synthesis to sites of cellular activity and to stimulate the local synthesis of phosphorylated forms of PI. The participation of various phosphoinositides in EGF signal transduction and in the trafficking of the EGF receptors is well documented. Using fluorescence lifetime imaging microscopy (FLIM) to measure fluorescence resonance energy transfer (FRET) between EGFP-PITP proteins and fluorescently labeled phospholipids, we report that PITPalpha and PITPbeta can dynamically interact with PI or PC at the plasma membrane when stimulated with EGF. Additionally, PITPbeta is localized at the Golgi, and EGF stimulation resulted in enhanced FRET. Inhibitors of the PLC and the Ras/MAP kinase pathway were both able to inhibit the EGF-stimulated interaction of PITPalpha with PI at the plasma membrane. The mobility of PITP proteins was determined by using fluorescence recovery after photobleaching (FRAP), and EGF stimulation reduced the mobility at the plasma membrane. We conclude that the dynamic behavior of PITPalpha and PITPbeta in vivo is a regulated process involving multiple mechanisms.


Subject(s)
Carrier Proteins/metabolism , Epidermal Growth Factor/metabolism , MAP Kinase Signaling System , Membrane Proteins/metabolism , Organic Chemicals , Type C Phospholipases/antagonists & inhibitors , ras Proteins/metabolism , Animals , Boron Compounds/chemistry , Butadienes/pharmacology , COS Cells/drug effects , Carrier Proteins/drug effects , Carrier Proteins/genetics , Cell Membrane/drug effects , Cell Membrane/metabolism , Enzyme Inhibitors/pharmacology , Epidermal Growth Factor/pharmacology , Fluorescence Resonance Energy Transfer , Golgi Apparatus/metabolism , Green Fluorescent Proteins , Lipid Metabolism , Lipids/chemistry , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , MAP Kinase Signaling System/drug effects , Membrane Proteins/drug effects , Membrane Proteins/genetics , Mitogen-Activated Protein Kinases/antagonists & inhibitors , Mutation , Nitriles/pharmacology , Phosphatidylcholines/metabolism , Phosphatidylethanolamines/metabolism , Phospholipid Transfer Proteins , Type C Phospholipases/metabolism , ras Proteins/drug effects
8.
Mol Biol Cell ; 13(10): 3730-46, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12388770

ABSTRACT

Phospholipase Ds (PLDs) are regulated enzymes that generate phosphatidic acid (PA), a putative second messenger implicated in the regulation of vesicular trafficking and cytoskeletal reorganization. Mast cells, when stimulated with antigen, show a dramatic alteration in their cytoskeleton and also release their secretory granules by exocytosis. Butan-1-ol, which diverts the production of PA generated by PLD to the corresponding phosphatidylalcohol, was found to inhibit membrane ruffling when added together with antigen or when added after antigen. Inhibition by butan-1-ol was completely reversible because removal of butan-1-ol restored membrane ruffling. Measurements of PLD activation by antigen indicate a requirement for continual PA production during membrane ruffling, which was maintained for at least 30 min. PLD1 and PLD2 are both expressed in mast cells and green fluorescent protein-tagged proteins were used to identify PLD2 localizing to membrane ruffles of antigen-stimulated mast cells together with endogenous ADP ribosylation factor 6 (ARF6). In contrast, green fluorescent protein-PLD1 localized to intracellular vesicles and remained in this location after stimulation with antigen. Membrane ruffling was independent of exocytosis of secretory granules because phorbol 12-myristate 13-acetate increased membrane ruffling in the absence of exocytosis. Antigen or phorbol 12-myristate 13-acetate stimulation increased both PLD1 and PLD2 activity when expressed individually in RBL-2H3 cells. Although basal activity of PLD2-overexpressing cells is very high, membrane ruffling was still dependent on antigen stimulation. In permeabilized cells, antigen-stimulated phosphatidylinositol(4,5)bisphosphate synthesis was dependent on both ARF6 and PA generated from PLD. We conclude that both activation of ARF6 by antigen and a continual PLD2 activity are essential for local phosphatidylinositol(4,5)bisphosphate generation that regulates dynamic actin cytoskeletal rearrangements.


Subject(s)
Antigens/metabolism , Cell Surface Extensions , Mast Cells/physiology , Phosphatidic Acids/metabolism , Phospholipase D/metabolism , ADP-Ribosylation Factor 1/metabolism , ADP-Ribosylation Factor 6 , ADP-Ribosylation Factors/metabolism , Actins/metabolism , Animals , Antigens/immunology , Butanols/pharmacology , Cells, Cultured , Cytoskeleton/metabolism , Enzyme Activation , Fluorescent Dyes/metabolism , Mast Cells/cytology , Mast Cells/drug effects , Mast Cells/immunology , Microscopy, Phase-Contrast , Phospholipase D/genetics , Protein Isoforms/metabolism , Rats , Recombinant Fusion Proteins/metabolism , Time Factors
9.
FEBS Lett ; 531(1): 74-80, 2002 Oct 30.
Article in English | MEDLINE | ID: mdl-12401207

ABSTRACT

Monomeric transport of lipids is carried out by a class of proteins that can shield a lipid from the aqueous environment by binding the lipid in a hydrophobic cavity. One such group of proteins is the phosphatidylinositol transfer proteins (PITP) that can bind phosphatidylinositol and phosphatidylcholine and transfer them from one membrane compartment to another. PITPs are found in both unicellular and multicellular organisms but not bacteria. In mice and humans, the PITP domain responsible for lipid transfer is found in five proteins, which can be classified into two classes based on sequence. Class I PITPs comprises two family members, alpha and beta, small 35 kDa proteins with a single PITP domain which are ubiquitously expressed. Class IIA PITPs (RdgBalphaI and II) are larger proteins possessing additional domains that target the protein to membranes and are only able to bind lipids but not mediate transfer. Finally, Class IIB PITP (RdgBbeta) is similar to Class I in size (38 kDa) and is also ubiquitously expressed. Class III PITPs, exemplified by the Sec14p family, are found in yeast and plants but are unrelated in sequence and structure to Class I and Class II PITPs. In this review we discuss whether PITP proteins are passive transporters or are regulated proteins that are able to couple their transport and binding properties to specific biological functions including inositol lipid signalling and membrane turnover.


Subject(s)
Carrier Proteins/metabolism , Membrane Proteins/metabolism , Phosphatidylinositols/metabolism , Saccharomyces cerevisiae Proteins , Animals , Carrier Proteins/physiology , Cell Membrane/metabolism , Humans , Lipid Metabolism , Lipids/chemistry , Membrane Proteins/physiology , Mice , Mice, Knockout , Models, Biological , Models, Molecular , Phosphatidylinositol 3-Kinases/metabolism , Phospholipid Transfer Proteins , Protein Structure, Secondary , Protein Structure, Tertiary , Signal Transduction
10.
Biochem J ; 366(Pt 1): 23-34, 2002 Aug 15.
Article in English | MEDLINE | ID: mdl-12023904

ABSTRACT

Mammalian phosphatidylinositol transfer proteins (PITPs) alpha and beta, which share 77% identity, have been shown to exhibit distinct lipid-transfer activities. In addition to transferring phosphatidylinositol (PI) and phosphatidylcholine (PC), PITPbeta has been shown to transfer sphingomyelin (SM), and this has led to the suggestion that PITPbeta is important for the regulation of SM metabolism. In the present study, we have analysed the ability of human PITPbeta to transfer and regulate the metabolism of cellular SM. We report that, in vitro, the two PITP isoforms were comparable in mediating PI, PC or SM transfer. Using permeabilized HL-60 cells as the donor compartment, both PITP isoforms efficiently transferred PI and PC, and were slightly active towards SM, with the activity of PITPbeta being slightly greater. To identify which cellular lipids were selected by PITPs, PITPalpha and PITPbeta were exposed to permeabilized HL-60 cells, and subsequently repurified and analysed for their bound lipids. Both PITPs were able to select only PI and PC, but not SM. SM synthesis takes place at the Golgi, and PITPbeta was shown to localize in that compartment. To examine the role of PITPbeta in SM biosynthesis, Golgi membranes were used. Purified Golgi membranes had lost their endogenous PITPbeta, but were able to recruit PITPbeta when added exogenously. However, PITPbeta did not enhance the activities of either SM synthase or glucosylceramide synthase. Further analysis in COS-7 cells overexpressing PITPbeta showed no effects on (a) SM and glucosylceramide biosynthesis, (b) diacylglycerol or ceramide levels, (c) SM transport from the Golgi to the plasma membrane, or (d) resynthesis of SM after exogenous sphingomyelinase treatment. Altogether, these observations do not support the suggestion that PITPbeta participates in the transfer of SM, the regulation of SM biosynthesis or its intracellular trafficking.


Subject(s)
Carrier Proteins/chemistry , Carrier Proteins/metabolism , Membrane Proteins , Sphingomyelins/biosynthesis , Sphingomyelins/metabolism , Animals , Biological Transport , Blotting, Western , COS Cells , Dose-Response Relationship, Drug , Golgi Apparatus/metabolism , HL-60 Cells , Humans , Hydrolysis , Lipid Metabolism , Phosphatidylcholines/metabolism , Phospholipid Transfer Proteins , Protein Isoforms/chemistry , Protein Isoforms/metabolism , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...