ABSTRACT
We present a method that combines the extraction of polychlorinated biphenyls (PCBs) and their separation from relatively large quantities of fat in biological matrixes by combined supercritical fluid extraction and separation. Cyano-functionalized silica gel, silica gel, aluminum oxide, Florisil, 3-amino-propyl-functionalized silica gel, and octadecyl-functionalized silica gel were tested for suitability as chromatographic media for separation of PCBs from lipids. Silica gel, 3-aminopropyl-functionalized silica gel, and Florisil adequately separated PCBs from lipids when eluted with supercritical CO2. Florisil allowed both the extraction of PCBs and their separation from lipids in PCB-spiked chicken egg and fish. Two grams of sample containing PCBs at 0.125 microgram/g was sufficient for subsequent separations and the low-level analysis required for the more toxic PCB components.
Subject(s)
Fats/analysis , Polychlorinated Biphenyls/analysis , Animals , Chemistry Techniques, Analytical/methods , Chickens , Eggs/analysis , Fishes , Lipids/analysis , Polychlorinated Biphenyls/isolation & purification , SolventsABSTRACT
The hepatic induction of cytochrome P-450-dependent monooxygenase components by cis- and trans-5,10-dihydrogen mirex was studied in male and female laboratory rats. There were sex-dependent differences between the two isomeric derivatives of mirex. The cis-isomer significantly increased aniline hydroxylase activity in the female, but not in the male. In contrast, aminopyrine N-demethylase was significantly increased by the cis-isomer in both sexes. The trans-isomer increased the hydroxylase and N-demethylase activities in both sexes. The cis-isomer induced NADPH-cytochrome c reductase in the female, and the trans-isomer did not. Both isomers induced hepatic reductase activity in the male rat. No sex-dependent differences in the hepatic induction of cytochrome P-450 were observed for either isomer.
