[Characterization of antagonistic and acid formation properties of Lactobacillus casei].

Cultural, morphologic, and biochemical characteristics of Lactobacillus casei were studied as well as their acid-forming and antagonistic activity and resistance to antibiotics. 60 cultures identified as L. caseiwere isolated from 250 samples of sour-milk products and clinical specimens. All isolated strains had respectively high antagonistic activity regardless of their source. Dependence between antagonistic activity and acid formation was not detected. Conclusion about promise for using these lactobacilli for manufacturing of probiotics has been done.

[The characteristics of the colonization resistance of the intestines in conventional animals with a history of clinical death]. / Kharakteristika kolonizatsionnoi rezistentnosti kishechnika konventsional'nykh zhivotnykh, perenesshikh klinicheskuiu smert'.

After modeling the terminal state caused by the acute loss of blood in rats their intestinal microflora was studied, as was their resistance to colonization. Decreased resistance to colonization was registered early after resuscitation (up to 3 days), which was confirmed by the translocation of bacteria into internal organs, decreased number of lactobacilli in the contents of the small intestine and elevated level of enterobacteria in the intestine. Disturbances in resistance to colonization was also manifested by prolonged colonization of the digestive tract of the resuscitated animals by Escherichia coli indicator strain K12pSS-120 carrying Shigella sonnei (phase I) invasiveness plasmid.

[The prevention of the translocation of intestinal microflora after the rescue of an organism from a terminal state]. / Profilaktika translokatsii kishechnoi mikroflory posle vyvedeniia organizma iz terminal'nogo sostoianiia.

In experiments on guinea pigs, the animals were resuscitated from clinical death caused by the acute loss of blood and subsequently treated intragastrically with enterosorbents: activated carbon fibrous material, alone and in combination with polymyxin B, polyphepan (a lignin derivative), polymethyloxan hydrogel and the sorbent Enterocat. In the animals, not treated during the postresuscitation period, a high population level of enterobacteria and Gram-positive aerobic cocci was registered in the contents of the small and large intestines and their translocation to mesenteric lymph nodes, liver, spleen and blood was observed. The amount of lactobacilli in the small intestine was decreased. Enterosorbents were found to decrease a high population level of intestinal microflora, to prevent the translocation of Gram-positive aerobic cocci and to inhibit the penetration of enterobacteria through the enteric barrier in the postresuscitation period. Combined use of activated carbon fibrous material with polymyxin B proved to be most effective for the elimination of enterobacteria.

[Immunomorphological characteristics of mesenteric lymph nodes and lymphoid nodules of the intestines of animals after clinical death]. / Immunomorfologicheskaia kharakteristika bryzheechnykh limfaticheskikh uzlov i limfoidnykh uzelkov kishechnika zhivotnykh, perenesshikh klinicheskuiu smert'.

The results obtained allow to determine, that the pathomorphologic changes (the hemorrhages, the stasis, the edema, the reduction of lymphoid tissue), the quantitative changes of immunoglobulin's cells in the intestine lymphoid tissue were detected. These influences had observed the persistence of enterobacteria, that was shown to observe the translocation from the intestine to the mesenterial lymph nodes during two weeks of postresuscitation period.

[Morphological characteristics of the small intestine and translocation of intestinal microflora in the post-resuscitation period]. / Morfologicheskaia kharakteristika tonkoi kishki i translokatsiia kishechnoi mikroflory v postreanimatsionnom periode.

Morphologic investigations were studied in rats during 1-3 hours after clinical death of the acute hemorrhage. The combination of morphologic and microbiologic methods obtained allow to describe the destruction of intestine wall and translocation of bacteria in the tissue and organs. In 3 days after clinical death the structure of small intestine regenerates, but the vital bacteria were isolated in the tissues and organs.

[The modelling of the translocation of the intestinal microflora in conventional animals]. / Modelirovanie translokatsii kishechnoi mikroflory na konventsional'nykh zhivotnykh.

To study the mechanisms of the translocation of enteric microflora and to develop the corresponding prophylactic measures, experimental models are necessary. In this connection two methods of simulating translocation are proposed. One of these methods is reproduced in animals resuscitated from the state of apparent death caused by acute venous blood loss and the other method, in animals with acute renal insufficiency induced by the ligation of the middle part of both ureters. During the period from the first hours to two weeks after the state of apparent death enterobacteria penetrate into mesenteric lymph nodes and occasionally into the liver, spleen and blood. On day 3 after the ligation of the ureters enterobacteria can be isolated from the above-mentioned organs and the blood, while the kidneys remain sterile. The models are reproducible, exhibit adequate clinical manifestations of these terminal states, and permit the detailed investigation of the translocation mechanisms.

[The autologous intestinal microflora in the postresuscitation period after acute blood loss in rats]. / Automikroflora kishechnika v postreanimatsionnom periode posle ostroi krovopoteri u krys.

In this work the data obtained in the study of intestinal microbiocenosis in rats on the third day of the postresuscitation period after acute blood loss are presented. The quantitative and qualitative shifts of microflora at different biotopes, such as the wall of the small intestine, the parietal microflora of Peyer's patches, the contents and wall of the large intestine, have been characterized. Some specific features of dysbiotic changes have been revealed in comparison with the shifts of intestinal microflora in cases of dysbacteriosis caused by other reasons. The translocation of different microorganisms, including Bacterium bifidum and lactobacilli, into mesenteric lymph nodes, the liver, the spleen and the blood has been observed.