Cytological and microbiological investigations of professional hygiene efficiency in patients with generalized periodontitis.

OBJECTIVE: Aim: The purpose of this study is to assess the impact of occupational hygiene procedures for microbiological and cytological contents of periodontal pockets. PATIENTS AND METHODS: Material and Methods: Cytological and microbiological content of the periodontal pockets before treatment and after professional hygiene procedures including scaling with hand instruments and root cementum polishing have been investigated in patients with periodontitis. RESULTS: Results: According to obtained data it can be resumed that in periodontitis patients with the depth of pockets 3-5,5 mm before professional hygiene all the pockets contain great number of Cocci, Spirochetes, Candida Albicans, Flagellated rods and Protozoa species. It was proved by revealing of small amount of Polymorphonuclear leukocytes with active phagocytosis. After scaling and planing of the roots, a decrease in the number of Protozoa and Candida Albicans was observed in 97% and 72% of the investigated cells, respectively. CONCLUSION: Conclusions: Cytological and microbiological content of periodontal pockets before treatment and after professional hygiene procedures including scaling and root planning testify to the level of local protective mechanisms, especially process of phagocytosis and virulence of microbial species in periodontal pockets.

The influence of immediate intraoperative loading with a splinting component on supporting tissues during a one-stage implant.

OBJECTIVE: Aim: To study the specifics of the impact of immediate intraoperative loading with a splinting component on supporting tissues during a one-stage implantation protocol. PATIENTS AND METHODS: Materials and Methods: In the course of the study, orthopedic treatment was carried out for 55 patients aged 29 to 60 years. The following were performed: cone-beam computed tomography, software planning and intraoral scanning with an optical scanner, one-stage implantation protocol, assessment of implant stability with the Osstell ISQ device, microcirculation study in the peri-implant area using laser Doppler flowmetry (LDF). RESULTS: Results: It was established that around loaded implants there is an increase in blood flow and vasomotor activity of the microcirculatory channel of the supporting tissues, an increase in the volume of bone tissue and an increase in torque, which is the optimal forecast for the acceleration of the pace of osseointegration. CONCLUSION: Conclusions: The use of a splinting component during immediate intraoperative functional masticatory load accelerates the dynamics of bone tissue remodeling processes around the implant, which is an optimal prediction of osseointegration rates in various dental implantation protocols and is consistent with high values of the implant stability coefficient.

EVALUATION OF DYNAMIC CHANGES IN THE MICROCIRCULATION OF THE MUCOSA IN THE ZONE OF DENTAL IMPLANTATION WITH IMMEDIATE INTRAOPERATIVE LOAD.

OBJECTIVE: The aim: Study of the dynamics of changes in the average values of the index of mucosal microcirculation after dental implantation with immediate intraoperative prosthetics. PATIENTS AND METHODS: Materials and methods: In clinical conditions, 55 patients aged from 29 to 60 years with a diagnosis of partial absence of teeth requiring orthopedic treatment using implants on the lower jaw were treated and examined. In the course of the latest achievements, the following methods were used: clinical protocol of immediate implantation with Solidum and Simplex implants of the «ART IMPLANT¼ system on the lower jaw by the one-stage implantation method, with immediate intraoperative loading and the manufacture of a temporary non-removable dental prosthesis, determination of the microcirculation index in dynamics using the laser Doppler method flowmetry, statistical analysis. RESULTS: Results: The obtained results indicate a pronounced reaction of microcirculation up to the 3rd day after surgery, an increase in blood perfusion of the mucous membrane by 2.7 times while maintaining vasomotor activity, which indicates adaptation to the injury and immediate loading of the denture in the postoperative period. 3 months after dental surgery and immediate intraoperative prosthetics, all indicators of microcirculation approach the initial values before surgery. CONCLUSION: Conclusions: With the help of laser Doppler flowmetry, the fact of a sharp restoration of microcirculation after dental implantation surgery with immediate intraoperative prosthetics is confirmed.

CHANGES IN THE ULTRASTRUCTURAL ELEMENTS OF PERIODONTAL NEUROTROPHY UNDER CONDITIONS OF ACUTE SIMPLE COAGULATION DYSTROPHY IN THE EXPERIMENT.

OBJECTIVE: Aim: To determine the role of damage to the ultrastructural elements of the periodontal nervous system in the pathogenesis of dystrophic periodontal disease. PATIENTS AND METHODS: Materials and Methods: The basis of the experimental part of the study was the preparation of ultrathin sections from blocks of gum tissue of white rats, which were prepared using the UMTP-3M device. The study and analysis of biopsy samples was carried out with the help of an electron microscope UEMV-100K. RESULTS: Results: With the help of transmission electron microscopy, it was found that from the first minutes after the injection of hemolysate of isogenic erythrocytes into the rats, aggregates of erythrocytes, clumps of blood plasma, clusters of fibrin monomer masses, bundles of fibrin fibers, platelet and homogeneous were present in the connective tissue of the gums, and in particular in the lumens of hemocapillaries microthrombi, which confirms damage to the ultrastructures of the periodontium, which lead to the development of a pathological process, which is described when simple coagulation dystrophy is reproduced. CONCLUSION: Conclusions: Coagulative damage to the ultrastructural elements of the periodontal nervous system is one of the important factors in the pathogenesis of dystrophic periodontal damage. Under these conditions, trophic disturbances occur, similar to those that occur when the integrity of the nerve is disturbed - neurotrophic mechanism of dystrophy.

COMPARATIVE EVALUATION OF CLINICAL APPLICATION OF MONOLITHIC AND FOLDING IMPLANTS IN REHABILITATION OF ELDERLY PATIENTS WITH VARIOUS DEGREES OF ATROPHY OF ALVEOLAR PROCESSES.

OBJECTIVE: The aim: Comparative evaluation of long-term results of clinical application of one- and two-stage surgical protocols of dental implantation with the use of monolithic and collapsible implants in the rehabilitation of elderly patients. PATIENTS AND METHODS: Materials and methods: Under clinical observation were 46 patients with various clinical diagnoses of dentition defects aged 60 to 70 years. The following methods were used in the study: one - stage surgical protocol of dental implantation operation with non - detachable implants of ART IMPLANT system with subsequent temporary splint fixed prosthesis and immediate occlusive functional load, mechanical oscillatory - resonance method, questionnaire and statistical analysis. RESULTS: Results: The duration of surgical stages of treatment and complete rehabilitation showed statistically significant differences (p <0.05) and was significantly less when using a single-stage protocol of dental implant surgery and non-detachable implants and averaged 3.9 ± 0.8, p <0.05 months against 7.3 ± 1.2, p <0.05 months in implants according to the two-stage protocol. Assessment of patient satisfaction with the treatment was directly correlated with his timing. CONCLUSION: Conclusions: Thus, it should be noted that the clinical use of one-stage surgical protocol of implantation and non-detachable (monolithic) dental implants of the system «ART IMPLANT¼ in the rehabilitation of elderly patients with varying degrees of atrophy of the alveolar processes of the jaws is clinically justified.

IMMEDIATE IMPLANTATION AND AESTHETIC COMPONENT AS A RESULT OF SUCCESSFUL FORECAST TREATMENT.

OBJECTIVE: The aim: Improving the method of immediate implantation in the aesthetic zone in case of bone deficiency to obtain the highest aesthetic and predictable treatment result. PATIENTS AND METHODS: Materials and methods: Under clinical observation were 32 patients with different clinical diagnoses in the anterior part of the upper jaw aged 30 to 55 years. In the course of recent advances, the following methods have been used: clinical protocol of immediate implantation with passive exceptional loads by temporary orthopedic constructions, X-ray method using cone-beam computed tomography, statistical analysis. RESULTS: Results: After surgical treatment of patients 1 year after surgery, the distribution of biotypes was as follows: in group 1 - thick biotype 12.87%, medium - 87.13%; in group 2 - thick biotype 27.04%, medium - 72.96%, with p <0.05. According to the results of CT, the distance between the implant and the vestibular in the first group was after 6 months - 1.67 ± 0.04 mm (p <0.05); in the second group of the study we obtained the following results after 6 months - 1.59 ± 0.06 mm (p <0.05). CONCLUSION: Conclusions: The advanced method of immediate implantation in the anterior part of the upper jaw allows to change the biotype of soft tissues, improve the color spectrum of the gums, increase the thickness of soft tissues with connective tissue autograft, and increase gum density and fixation of osteoplastic material in the presence of defect ), as well as reduce the risk of recession.

EXPERIMENTAL STUDY OF BONE REBUILDING IN THE PERIIMPLANTATION AREA UNDER IMMEDIATE LOADING ON DENTAL IMPLANTS.

OBJECTIVE: The aim: Study of the dynamics of morphological rearrangement of bone under conditions of immediate occlusive functional load and the effect of splinting of implants with temporary orthopedic structures with the analysis of the coefficient of stability of implants during immediate implantation in the experiment. PATIENTS AND METHODS: Materials and methods: A series of experiments was performed on 6 male Duroc pigs at the age of 6 months and weighing 40-60 kg. In the course of recent advances, the following methods have been used: the clinical protocol of immediate - implantation of time-consuming clothes, the definition of COEFICIENT, morphometry and light microscopy of the slides, statistical analysis. RESULTS: Results: By morphometric examination after 3 months the BIC in the series with splinting was 1.68 times higher compared to 1 month. studies, in a series of experiments without splinting - 1.9 times, after 3 months. the difference between implantation experiments with splinting components and without splinting is 1.6 times. During the functional study of the resonant - frequency analyzer, there is an increase in the ISQ in the second and third months after surgery, but this figure is higher in the study using the splint component. CONCLUSION: Conclusions: Stagnation of the shingle component in the case of intrinsic intraoperative functional juvenile implantation accelerates the dynamics of osteointegration, so that high indicators of the efficiency of the implant stability can be achieved.

The use of photodynamic therapy in the treatment of dental caries in children of contaminated areas of the ecosystem of the upper tysa region.

OBJECTIVE: The aim: improving the effectiveness of treatment of dental caries in children using the method of photodynamic therapy. PATIENTS AND METHODS: Materials and methods: The treatment of permanent tooth decay was performed in 35 children of the Upper Tysa region aged 12-15 years. Using the polymerase chain reaction method, the clinical efficacy of photodynamic therapy in the treatment of dentin caries was determined. RESULTS: Results: During the study, by PCR analysis, dentin caries most commonly revealed genetic markers of DNA of the five most virulent anaerobic bacteria: Prevotella intermedia, Fusobacterium spp., Enterococcus Faecalis, Veilonella spp., Candida albicans. After treatment of the carious cavity by the method of photodynamic therapy with exposure of 30 s - fusobacterium spp. were not detected, the detection rate of prevotella intermedia decreased 3-fold, enterococcus faecalis 3.5-fold, veilonella spp.- 5-fold, and candida albicans- 8-fold; after treatment with carious cavities with 60 s exposure - anaerobic microorganisms were not detected in the investigated samples. The effect of photodynamic therapy with laser exposure of 60 seconds on caries-causing streptococci resulted in their total death, and with exposure of 30 seconds - the frequency of isolated strains decreased several folds. CONCLUSION: Conclusions: The use of photodynamic therapy in the treatment of dentine caries is a highly effective and pathogenetically sound method of treatment that provides a significant reduction in the optional and obligate types of cariesogenic microorganisms.

Prevention of main dental diseases in children using herbal tea «dentesvita¼

Introduction: Transcarpathian region refers to the climate-geographic zone with a low level of fluoride and iodine in the environment and as shown by the epidemiological survey «very high¼ according to WHO criteria for the level of intensity of main dental diseases. With the purpose of directing the implementation of the state policy in the field of health care and for the prevention of dental diseases, the industrial release of a functional food product - «DentesVita¼ enriched with iodine and fluorine - has been adjusted. The aim: Was to study the effectiveness of the use of functional food product - DentesVita for the prevention of dental caries in children. Material and methods: A comprehensive dental examination of 180 children of 12 years of age has been carried out: 60 of them in each of the climatic and geographical zones of Transcarpathia: plain, foothills and mountains. The intensity of caries according to the DMF index, increase of intensity was determined. A simplified hygiene index (OHI-S) was used to assess the hygienic state of the oral cavity. Carried out research on the mineralization potential of saliva. Results: The growth of caries in children of the main group of the plain zone was 1.8 times smaller, and in the foothills and mountains 1.6 times smaller than the similar rates of control group children (p <0.05). The caries prophylactic effect at that was among schoolchildren in the plain zone: 43.48%, foothill - 38.66%, and mountainous - 23.53%. Conclusions: These studies confirm the effectiveness of using «DentesVita¼ for the prevention of dental caries in children living in conditions of biogeochemical deficiency of fluorine and iodine on the basis of caries intensity growth indexes, hygienic indexes of the oral cavity and mineralizing ability of oral liquid.