Evaluation of the time-dependent osteogenic activity of glycerol incorporated magnesium oxide nanoparticles in induced calvarial defects.

Introduction: Magnesium-based biomaterials have been explored for their potential as bone healing materials, as a result of their outstanding biodegradability and biocompatibility. These characteristics make magnesium oxide nanoparticles (MgO NPs) a promising material for treating bone disorders. The purpose of this investigation is to assess the osteogenic activity of newly-developed locally administered glycerol-incorporated MgO NPs (GIMgO NPs) in rabbits' calvarial defects. Materials and methods: Characterization of GIMgO was done by X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Bilateral calvarial defects were created in eighteen New Zealand Rabbits, of which they were divided into 3 groups with time points corresponding to 2, 4, and 6 weeks postoperatively (n = 6). One defect was implanted with absorbable gel foam impregnated with GIMgO NPs while the other was implanted with gel foam soaked with glycerol (the control). The defects were assessed using histological, Micro-Computed Tomography (Micro-CT), and histometric evaluation. Results: The characterization of the GIMgO nanogel revealed the presence of MgO NPs and glycerol as well as the formation of the crystalline phase of the MgO NPs within the nanogel sample. The histological and micro-CT analysis showed time-dependent improvement of healing activity in the calvarial defects implanted with GIMgO NPs when compared to the control. Furthermore, the histometric analysis demonstrated a marked increase in the total area of new bone, connective tissue, new bone area and volume in the GIMgO NPs implanted site. Statistically, the amount of new bone formation was more significant at 6 weeks than at 2 and 4 weeks postoperatively in the calvarial defects implanted with GIMgO NPs as compared to the control. Conclusion: The locally applied GIMgO NPs demonstrated efficacy in promoting bone formation, with more significant effects observed over an extended period. These findings suggest its suitability for clinical use as a therapeutic alternative to enhance bone healing.

Prevalence of apical periodontitis in endodontically-treated maxillary and mandibular posterior teeth in a Saudi Arabian population: a cone-beam computed tomography study.

OBJECTIVE: The aim of this retrospective study was to assess the prevalence of apical periodontitis (AP) in root canal-treated maxillary and mandibular posterior teeth in a Saudi Arabian population based on findings from images taken using cone-beam computed tomography (CBCT). METHODS: The sample included 300 CBCT images from patients (150 females and 150 males) aged between 18 and 80 years old and they were analyzed to detect AP in endodontically treated maxillary and mandibular premolars and molars. Also, the correlation between the prevalence of AP and gender along with location (right/left side of jaw) were analyzed. Periapical lesions were defined as lamina dura gaps appearing disrupted around the width of periodontal ligament and apex at the apical third of the roots. RESULTS: There was higher prevalence of AP in maxillary (19.1%) when compared to mandibular posterior teeth (17.3%). Mandibular first and maxillary second molars were the most affected teeth with AP (P = 0.05 and P = 0.04, respectively). Also, there was higher prevalence of AP cases with a predilection toward males (Maxillary P = 0.005; Mandibular P = 0.00) and on the right side of the mandibular jaw (P = 0.03). CONCLUSION: In this study, the prevalence of AP had a significant association between gender with a predilection in males and location with a tendency to occur on the mandibular right side as observed in endodontically treated maxillary and mandibular posterior teeth using CBCT.

Parental Knowledge and Awareness of Fluoride Varnish Application on Their Children - A Cross-Sectional Study.

Introduction: Fluoride varnishes are forms of topical fluoride that are applied on the surfaces of teeth to prevent from dental caries. It contains sodium fluoride, which is the active ingredient in the varnish and the concentration is about 22,600 ppm. Fluoride can promote enamel remineralization which protect against dental caries and it can be professionally applied by dentists and physicians for infants and young children. Methods: In this, cross-sectional study, a close-ended survey containing 14 items was given to 399 randomly assigned parents in Jeddah, Saudi Arabia. The questionnaire included demographic data and items to assess the general knowledge about fluoride and fluoride varnish. The responses were coded and statistical analysis was performed. Results: About 65.3% of males and 47.4% females were not sure if fluoride application was safe for their children, however, 67.2% of the population revealed that tooth pastes were one among the fluoride sources for their children. Pearson's correlation test showed high statistical significance between the parents' educational background and awareness level of fluoride varnish was of with p-value-0.000 and correlation coefficient (r-value-0.427). Conclusion: The lack of awareness among parents on an important caries-control measure warrants the implementation of special awareness programs on fluorides and its benefits in young children.

Microbial Culture and Scanning Electron Microscopic Evaluation of Endodontic Hand Files: An In Vitro Study.

Background Endodontic hand files are used during root canal treatment in the cleaning and shaping step of the procedure. Whether endodontic instruments should be single-use or reusable is a topic of debate. This in vitro study aimed to analyze the bioburden on used and new sterilized endodontic hand files. Methodology A total of 30 K-files (15 used, 15 new) and 30 H-files (15 used, 15 new) were studied. After clinical use, the used and new files were subjected to phloxine B staining, scanning electron microscope (SEM) analysis, and microbial culture. We used a Fisher's exact test to investigate the significant difference in contamination and bioburden between new and used endodontic files. Results The chi-square test showed no statistically significant difference between new and used groups in staining. In the used group, 20% of the H-files and 6.7% of the K-files were positive for bioburden (p > 0.05). The SEM analysis showed that all used files (100%) were contaminated with biological debris. All new files and most of the used files (86.7%) were negative for bacterial culture. Conclusions Used K-files and H-files (sizes 25 and below) had greater contamination and bioburden than new files. Our results highlight the inadequacy of cleaning methods (mechanical/ultrasonication pre-cleaning and heat sterilization) employed between re-usage of files in this study. Appropriate decision-making on either adapting an evidence-based and effective reprocessing strategy or single-use files can be considered by dentists.

Evidence-based Decisioning on the Management of a High Caries Risk Patient-A Case Report.

OBJECTIVE: The evidence-based approach to treatment planning has been at the forefront of clinical dentistry and the use of scientific evidence for clinical decisions has an impact on this case report, where the treatment planning of a high caries risk patient was performed with this approach. BACKGROUND: In this case report, management of a 30-year-old female patient with multiple carious lesions in her oral cavity has been discussed, and microbiological tests for caries risk assessment were performed that categorized her in the high caries risk group. METHODS: The management of this patient was formulated by carrying out a systematic search of the literature according to the clinical question. It is the evidence-based dentistry protocol, and the search was carried out in the following databases: PubMed, Embase, Medline, and Google Scholar. The CAMBRA protocol was employed for the management of high caries risk patients. RESULTS: A total of seven articles that included two randomized clinical trials, one case report, and four retrospective studies were identified for the evidence-based approach of the treatment planning. CONCLUSION: The caries management by risk assessment has been advocated in this case report, where the patient was identified in the high caries risk group according to the caries assessment tests (microbiological), and the management was carried out inculcating the CAMBRA protocol.

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments.

The reuse of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. A total of 122 HAs (used and sterilized [n = 107]; new [n = 15]) were procured from 3 centers, of which 3 samples were discarded due to perforation in the sterilization pouch. For sterility assessment, the used HAs (n = 80) were cultured in Brain Heart Infusion Broth (BHI) and potato dextrose agar (PDA); bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with phloxine B, photographed, and compared to new HAs (n = 5). A scanning electron microscope (SEM) assessed the differences between 2 sets of HAs, after which the 7 contaminated HAs along with 24 used HAs from staining experiment (total: 31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering reuse of HAs.

Awareness of the Effect of Diabetes on Oral Health among a Population in Jeddah, Saudi Arabia.

PURPOSE: Diabetes is an ever-growing health issue in the Kingdom of Saudi Arabia. It has several oral health implications and oral health in turn affects diabetes control. The primary objective of this research was to study the awareness of the effect of diabetes on oral health among the general population in the city of Jeddah, Saudi Arabia. MATERIALS AND METHODS: A closed-ended, validated questionnaire was distributed to 506 randomly selected shopping-mall-goers. Responses were coded and entered into spreadsheet (SPSS, IBM) and frequency distribution of the responses was calculated. RESULTS: The majority of the respondents were females (62.5%), non-diabetic (80.2%) and reported a positive family history of diabetes (87.9%). Most of them (63.4%) understood the importance of discussing one's diabetes status with the dentist as it affected the treatment plan, and also knew (84.4%) that diabetes affects oral health in some way. A majority also correctly responded to how diabetes affects oral health (66.3%) and to the sequelae of untreated gum disease (87.2%). The majority of the respondents had not received any tips or information regarding the connection between diabetes and oral health. CONCLUSION: This study reported adequate knowledge of the sample with respect to diabetes-related oral health. An important finding of this study was that the majority of the study participants did not receive information leading to diabetes-related oral health awareness or knowledge from anyone, which implies that health professionals and health media do not play the requisite role in dissemination of this important aspect of public health.

Awareness of population regarding the effects of diabetes on dental implant treatment in Jeddah, Saudi Arabia.

OBJECTIVE: Diabetes mellitus (DM) has several complications. Delayed wound healing, microvascular disease and an impaired response to infections are complications that can have a direct bearing on dental implant therapy. This paper studies the awareness of the population with regard to the effect of DM on dental implant treatment. MATERIALS AND METHODS: A validated, close-ended questionnaire was distributed to 506 randomly selected mall-goers in the city of Jeddah. Responses were coded and entered into spreadsheet software (SPSS, IBM). The frequency distribution of the responses was calculated, and inferences were drawn. RESULTS: The study revealed that the majority of the sample were females (62.8%), did not have diabetes (80.4%) and reported a positive family history of diabetes (87.4%). Most of the respondents (56%) believed that uncontrolled diabetes can lead to implant loss and that diabetes affects the healing process (91.6%). Many patients (42%) responded that diabetes could be treated with dental implants if the blood sugar level was controlled. CONCLUSION: The studied sample revealed a satisfactory level of awareness regarding the association of diabetes and oral hygiene in dental implant therapy. However, there is less than adequate knowledge about the effects of diabetes on dental implants, as the majority of the respondents believe that only controlled diabetics can avail dental implant treatment.

VWC2 Increases Bone Formation Through Inhibiting Activin Signaling.

By a bioinformatics approach, we have identified a novel cysteine knot protein member, VWC2 (von Willebrand factor C domain containing 2) previously known as Brorin. Since Brorin has been proposed to function as a bone morphogenetic protein (BMP) antagonist, we investigated the binding of Brorin/VWC2 to several BMPs; however, none of the BMPs tested were bound to VWC2. Instead, the ßA subunit of activin was found as a binding partner among transforming growth factor (TGF)-ß superfamily members. Here, we show that Vwc2 gene expression is temporally upregulated early in osteoblast differentiation, VWC2 protein is present in bone matrix, and localized at osteoblasts/osteocytes. Activin A-induced Smad2 phosphorylation was inhibited in the presence of exogenous VWC2 in MC3T3-E1 osteoblast cell line and primary osteoblasts. The effect of VWC2 on ex vivo cranial bone organ cultures treated with activin A was investigated, and bone morphometric parameters decreased by activin A were restored with VWC2. When we further investigated the biological mechanism how VWC2 inhibited the effects of activin A on bone formation, we found that the effects of activin A on osteoblast cell growth, differentiation, and mineralization were reversed by VWC2. Taken together, a novel secretory protein, VWC2 promotes bone formation by inhibiting Activin-Smad2 signaling pathway.

Biomarkers of alveolar bone resorption in gingival crevicular fluid: A systematic review.

BACKGROUND: Periodontitis is a prevalent oral disease with bone loss being it's hallmark. Clinical parameters used to measure periodontitis are retrospective and do not indicate active inflammation nor prognosis. GCF can be easily collected chairside and bone turnover biomarkers found in GCF can be evaluated to check for active inflammation and disease progression. This systematic review aims to evaluate the literature for association and predictive value of bone turnover biomarkers in GCF during periodontal disease. MATERIALS AND METHODS: This review was conducted and reported according to the PRISMA guidelines. The online databases Google Scholar and PubMed were used for data search. MeSH terms were used for PubMed search. All original studies from 1990 to 2017 conducted on human subjects in the English language were included in the review. Studies on non-human subjects, reviews and studies conducted in languages other than English were not considered. Reference lists of qualified articles were also searched. RESULTS: The search generated 2300 results whose titles were screened and 1571 articles were retreived. 23 articles were accepted in the review and full texts were accessed. These included 1 randomized controlled trial, 12 cross-sectional studies, five pre-post interventional studies, 4 longitudinal and 1 in-vitro in-vivo experimental study. The studies were conducted on patients of both genders ranging from 10 to 81 years in age. A total of 37 biomarkers were evalueted in the studies included in this review. Majority of the studies reported interleukin-1ß (IL-1ß) while receptor activated nuclear factor-kappa B ligand (RANKL) and matrix metalloproteinase-8 (MMP-8) were the other frequently reported biomarkers. Most of the studies evaluated more than two biomarkers. ELISA was the most commonly used biochemical test used for detection. CONCLUSION: A wide range of biomarkers have been established as indicators of alveolar bone resorption. Few of the biomarkers have also shown positive correlation with disease progression and outcome of periodontal therapies thus underscoring their predictive value in periodontal diagnosis and prognosis. Not one single biomarker has been reported to have a predictive advantage over another and a combination of two or more biomarkers along with clinical evaluation is recommended.