Relevance of the bacteriophage adherence to mucus model for Pseudomonas aeruginosa phages.

Pseudomonas aeruginosa infections are getting increasingly serious as antimicrobial resistance spreads. Phage therapy may be a solution to the problem, especially if improved by current advances on phage-host studies. As a mucosal pathogen, we hypothesize that P. aeruginosa and its phages are linked to the bacteriophage adherence to mucus (BAM) model. This means that phage-host interactions could be influenced by mucin presence, impacting the success of phage infections on the P. aeruginosa host and consequently leading to the protection of the metazoan host. By using a group of four different phages, we tested three important phenotypes associated with the BAM model: phage binding to mucin, phage growth in mucin-exposed hosts, and the influence of mucin on CRISPR immunity of the bacterium. Three of the tested phages significantly bound to mucin, while two had improved growth rates in mucin-exposed hosts. Improved phage growth was likely the result of phage exploitation of mucin-induced physiological changes in the host. We could not detect CRISPR activity in our system but identified two putative anti-CRISPR proteins coded by the phage. Overall, the differential responses seen for the phages tested show that the same bacterial species can be targeted by mucosal-associated phages or by phages not affected by mucus presence. In conclusion, the BAM model is relevant for phage-bacterium interactions in P. aeruginosa, opening new possibilities to improve phage therapy against this important pathogen by considering mucosal interaction dynamics.IMPORTANCESome bacteriophages are involved in a symbiotic relationship with animals, in which phages held in mucosal surfaces protect them from invading bacteria. Pseudomonas aeruginosa is one of the many bacterial pathogens threatening humankind during the current antimicrobial resistance crisis. Here, we have tested whether P. aeruginosa and its phages are affected by mucosal conditions. We discovered by using a collection of four phages that, indeed, mucosal interaction dynamics can be seen in this model. Three of the tested phages significantly bound to mucin, while two had improved growth rates in mucin-exposed hosts. These results link P. aeruginosa and its phages to the bacteriophage adherence to the mucus model and open opportunities to explore this to improve phage therapy, be it by exploiting the phenotypes detected or by actively selecting mucosal-adapted phages for treatment.

Morpho-Physiological Traits and Oil Quality in Drought-Tolerant Raphanus sativus L. Used for Biofuel Production.

Raphanus sativus L. is a potential source of raw material for biodiesel fuel due to the high oil content in its grains. In Brazil, this species is cultivated in the low rainfall off-season, which limits the productivity of the crop. The present study investigated the effects of water restriction on the physiological and biochemical responses, production components, and oil quality of R. sativus at different development stages. The treatments consisted of 100% water replacement (control), 66%, and 33% of field capacity during the phenological stages of vegetative growth, flowering, and grain filling. We evaluated characteristics of water relations, gas exchange, chlorophyll a fluorescence, chloroplast pigment, proline, and sugar content. The production components and chemical properties of the oil were also determined at the end of the harvest cycle. Drought tolerance of R. sativus was found to be mediated primarily during the vegetative growth stage by changes in photosynthetic metabolism, stability of photochemical efficiency, increased proline concentrations, and maintenance of tissue hydration. Grain filling was most sensitive to water limitation and showed a reduction in yield and oil content. However, the chemical composition of the oil was not altered by the water deficit. Our data suggest that R. sativus is a drought-tolerant species.

Sexual health and access to services for lesbian women in Manaus, Brazil. / Saúde sexual e acesso aos serviços para mulheres lésbicas em Manaus, Amazonas, Brasil.

This study aims to understand the meanings related to sexual health and the delineations that define the experience of accessing health services for lesbian women in Manaus, Brazil. This study followed a qualitative approach, counting on the participation of ten women who self-reported themselves as lesbians. Semi-structured interviews were carried out and their analyses occurred through three thematic axes. The first addressed the representations concerning prevention and sexual practices, highlighting the notion of fidelity in the relationship as a "protective factor". Difficulties in the use of condoms in relationships between two women were reported. The second discussed heteronormativity and its effects on self-care, reporting the participants' difficulties in being understood and welcomed by health services. The third addressed the search for one's own knowledge as a care tactic, highlighting the importance of information and autonomy for health promotion and prevention of Sexually Transmitted Infections (STIs). It can therefore be concluded that there is a need for public policies aimed at promoting the sexual health of lesbian women and the recognition of their specificities by health services.

O estudo tem por objetivo compreender os significados relacionados à saúde sexual e os contornos que definem a experiência de acesso aos serviços de saúde para mulheres lésbicas de Manaus, Amazonas. Trata-se de uma pesquisa de abordagem qualitativa. Contou com a participação de dez mulheres que se autoidentificaram como lésbicas. Foram realizadas entrevistas semiestruturadas e suas análises ocorreram por meio de três eixos temáticos. O primeiro abordou as representações sobre prevenção e práticas sexuais, destacando a noção de fidelidade no relacionamento como "fator de proteção". Foram relatadas dificuldades no uso de preservativos em relações entre duas mulheres. O segundo discutiu a heteronormatividade e seus efeitos no cuidado de si, relatando as dificuldades de as participantes serem compreendidas e acolhidas pelos serviços de saúde. O terceiro abordou a busca do próprio conhecimento como tática de cuidado, destacando a importância da informação e da autonomia para a promoção da saúde e a prevenção de infecções sexualmente transmissíveis. Conclui-se que existe a necessidade de políticas públicas voltadas para a promoção da saúde sexual de mulheres lésbicas e o reconhecimento de suas especificidades pelos serviços de saúde.

Meanings and perceptions of parenthood among transgender men who became pregnant before gender transition. / Sentidos e significados de parentalidade entre homens trans que engravidaram antes da transição de gênero.

This qualitative study, with five participating interlocutors, sought to understand the senses and meanings of parenting among trans men who became pregnant before gender transition. Analysis was conducted in light of social theories of gender. The results demonstrated an experience of parenthood subject to a field of tensions and negotiations, as well as subjective production that oscillated between transgression and accommodation of the cultural perspective of their own experience. The forms of violence found to be practiced reiterated social vulnerabilities, exposed healthcare service weaknesses and produced harmful effects on transgender men who become pregnant before gender transition.

Buscou-se compreender os sentidos e significados do exercício parental entre homens trans que engravidaram antes da transição de gênero por meio de uma pesquisa qualitativa, na qual participaram cinco interlocutores, cuja análise foi realizada à luz das teorias sociais de gênero. Os resultados demonstraram uma experiência parental sujeitada a um campo de tensão e negociações, além de uma produção subjetiva que oscilava entre a transgressão e a acomodação da perspectiva cultural da sua própria vivência. Identificou-se práticas de violências que reiteraram as vulnerabilidades sociais, deflagraram as fragilidades dos serviços de saúde e provocaram efeitos deletérios em homens trans que engravidam antes da transição de gênero.

Immune Response to an Inactivated Vaccine of SARS-CoV-2 (CoronaVac) in an Indigenous Brazilian Population: A Cohort Study.

INTRODUCTION: Although the adaptive immune responses to the CoronaVac vaccine are known, their dynamics in indigenous communities remain unclear. In this study, we assessed the humoral and cellular immune responses to CoronaVac (Sinovac Biotech Life Sciences, 2021 NCT05225285, Beijing, China), in immunized Brazilian indigenous individuals. METHODS: We conducted a prospective cohort study on indigenous Brazilian people between February 2021 and June 2021. Analyses of immune responses were carried out before (T1) and after a vaccination schedule was completed (T2). Demographic data were collected using a questionnaire. RESULTS: We initially included 328 patients; among them, 120 (36.6%) had no SARS-CoV-2 antibodies. Peripheral blood mononuclear cells (PBMCs) were collected from 106 patients during follow-up visits, of which 91 samples were analyzed by immunophenotyping assay to detect SARS-CoV-2-specific memory T-cell response. Post-vaccination, the levels of memory B-cells and Natural Killer T-lymphocytes increased. Bororó village residents, females, and Terena ethnic group members had higher levels of anti-spike IgG antibodies post-vaccination, whereas alcohol and tobacco users had lower concentrations. CONCLUSIONS: To our best knowledge, this was the first comprehensive assessment of antibody and T-cell responses against CoronaVac vaccination in indigenous patients. Our findings showed that antibody response and T-cell immunity against SARS-CoV-2 were present in most patients following the vaccination schedule.

Body fat predicts urinary tract infection in kidney transplant recipients: a prospective cohort study.

BACKGROUND: The association between obesity and infectious diseases is increasingly reported in the literature. There are scarce studies on the association between obesity and urinary tract infection after kidney transplantation (KTx). These studies defined obesity based on body mass index, and their results were conflicting. The present study aimed to evaluate this association using bioelectrical impedance analysis for body composition evaluation, and obesity definition. METHODS: A single-center cohort study was conducted. Demographic, clinical, anthropometric, and laboratory data were collected at KTx admission, and bioelectrical impedance analysis was performed to measure the visceral fat area, waist circumference, and total fat mass. The occurrence of urinary tract infection (symptomatic bacteriuria and/or histological evidence of pyelonephritis) was evaluated within three months after KTx. RESULTS: Seventy-seven patients were included in the cohort, and 67 were included in the final analysis. Urinary tract infection was diagnosed in 23.9% of the transplanted patients. Waist circumference (HR: 1.053; 95% CI 1.005-1.104; p = 0.032), visceral fat area (HR: 1.015; 95% CI 1.003-1.027; p = 0.014), and total fat mass (HR: 1.075; 95% CI 1.008-1.146; p = 0.028) were associated with urinary tract infection occurrence after KTx, using Cox regression models. Patients with high waist circumference (above 102 cm for men and above 88 cm for women) had a 4.7 times higher risk of a urinary tract infection than those with normal waist circumference (HR: 4.726; 95% CI 1.267-17.630; p = 0.021). Kaplan-Meier curves showed that patients with high waist circumference, high visceral fat area, and high total fat mass had more urinary tract infections (Log-rank test p = 0.014, p = 0.020, and p = 0.018, respectively). Body mass index was not able to predict urinary tract infection in the study sample. CONCLUSIONS: Waist circumference, visceral fat area, and total fat mass, assessed by bioelectrical impedance analysis, were predictors of urinary tract infection risk within the first three months after KTx.

The consequences of viral infection on protists.

Protists encompass a vast widely distributed group of organisms, surpassing the diversity observed in metazoans. Their diverse ecological niches and life forms are intriguing characteristics that render them valuable subjects for in-depth cell biology studies. Throughout history, viruses have played a pivotal role in elucidating complex cellular processes, particularly in the context of cellular responses to viral infections. In this comprehensive review, we provide an overview of the cellular alterations that are triggered in specific hosts following different viral infections and explore intricate biological interactions observed in experimental conditions using different host-pathogen groups.

Sentidos e significados de parentalidade entre homens trans que engravidaram antes da transição de gênero / Meanings and perceptions of parenthood among transgender men who became pregnant before gender transition

Resumo Buscou-se compreender os sentidos e significados do exercício parental entre homens trans que engravidaram antes da transição de gênero por meio de uma pesquisa qualitativa, na qual participaram cinco interlocutores, cuja análise foi realizada à luz das teorias sociais de gênero. Os resultados demonstraram uma experiência parental sujeitada a um campo de tensão e negociações, além de uma produção subjetiva que oscilava entre a transgressão e a acomodação da perspectiva cultural da sua própria vivência. Identificou-se práticas de violências que reiteraram as vulnerabilidades sociais, deflagraram as fragilidades dos serviços de saúde e provocaram efeitos deletérios em homens trans que engravidam antes da transição de gênero.

Abstract This qualitative study, with five participating interlocutors, sought to understand the senses and meanings of parenting among trans men who became pregnant before gender transition. Analysis was conducted in light of social theories of gender. The results demonstrated an experience of parenthood subject to a field of tensions and negotiations, as well as subjective production that oscillated between transgression and accommodation of the cultural perspective of their own experience. The forms of violence found to be practiced reiterated social vulnerabilities, exposed healthcare service weaknesses and produced harmful effects on transgender men who become pregnant before gender transition.

Saúde sexual e acesso aos serviços para mulheres lésbicas em Manaus, Amazonas, Brasil / Sexual health and access to services for lesbian women in Manaus, Brazil

Resumo O estudo tem por objetivo compreender os significados relacionados à saúde sexual e os contornos que definem a experiência de acesso aos serviços de saúde para mulheres lésbicas de Manaus, Amazonas. Trata-se de uma pesquisa de abordagem qualitativa. Contou com a participação de dez mulheres que se autoidentificaram como lésbicas. Foram realizadas entrevistas semiestruturadas e suas análises ocorreram por meio de três eixos temáticos. O primeiro abordou as representações sobre prevenção e práticas sexuais, destacando a noção de fidelidade no relacionamento como "fator de proteção". Foram relatadas dificuldades no uso de preservativos em relações entre duas mulheres. O segundo discutiu a heteronormatividade e seus efeitos no cuidado de si, relatando as dificuldades de as participantes serem compreendidas e acolhidas pelos serviços de saúde. O terceiro abordou a busca do próprio conhecimento como tática de cuidado, destacando a importância da informação e da autonomia para a promoção da saúde e a prevenção de infecções sexualmente transmissíveis. Conclui-se que existe a necessidade de políticas públicas voltadas para a promoção da saúde sexual de mulheres lésbicas e o reconhecimento de suas especificidades pelos serviços de saúde.

Abstract This study aims to understand the meanings related to sexual health and the delineations that define the experience of accessing health services for lesbian women in Manaus, Brazil. This study followed a qualitative approach, counting on the participation of ten women who self-reported themselves as lesbians. Semi-structured interviews were carried out and their analyses occurred through three thematic axes. The first addressed the representations concerning prevention and sexual practices, highlighting the notion of fidelity in the relationship as a "protective factor". Difficulties in the use of condoms in relationships between two women were reported. The second discussed heteronormativity and its effects on self-care, reporting the participants' difficulties in being understood and welcomed by health services. The third addressed the search for one's own knowledge as a care tactic, highlighting the importance of information and autonomy for health promotion and prevention of Sexually Transmitted Infections (STIs). It can therefore be concluded that there is a need for public policies aimed at promoting the sexual health of lesbian women and the recognition of their specificities by health services.

Dynamic transmission modeling of COVID-19 to support decision-making in Brazil: A scoping review in the pre-vaccine era.

Brazil was one of the countries most affected during the first year of the COVID-19 pandemic, in a pre-vaccine era, and mathematical and statistical models were used in decision-making and public policies to mitigate and suppress SARS-CoV-2 dispersion. In this article, we intend to overview the modeling for COVID-19 in Brazil, focusing on the first 18 months of the pandemic. We conducted a scoping review and searched for studies on infectious disease modeling methods in peer-reviewed journals and gray literature, published between January 01, 2020, and June 2, 2021, reporting real-world or scenario-based COVID-19 modeling for Brazil. We included 81 studies, most corresponding to published articles produced in Brazilian institutions. The models were dynamic and deterministic in the majority. The predominant model type was compartmental, but other models were also found. The main modeling objectives were to analyze epidemiological scenarios (testing interventions' effectiveness) and to project short and long-term predictions, while few articles performed economic impact analysis. Estimations of the R0 and transmission rates or projections regarding the course of the epidemic figured as major, especially at the beginning of the crisis. However, several other outputs were forecasted, such as the isolation/quarantine effect on transmission, hospital facilities required, secondary cases caused by infected children, and the economic effects of the pandemic. This study reveals numerous articles with shared objectives and similar methods and data sources. We observed a deficiency in addressing social inequities in the Brazilian context within the utilized models, which may also be expected in several low- and middle-income countries with significant social disparities. We conclude that the models were of great relevance in the pandemic scenario of COVID-19. Nevertheless, efforts could be better planned and executed with improved institutional organization, dialogue among research groups, increased interaction between modelers and epidemiologists, and establishment of a sustainable cooperation network.

Seroprevalence Of SARS-COV-2 infection in asymptomatic indigenous from the largest Brazilian periurban area.

This study assessed the seroprevalence of SARS-CoV-2 in 496 asymptomatic individuals from Mato Grosso do Sul, located in Dourados, the largest periurban indigenous area in Brazil, from January 25 to February 4, 2021. The volunteers participated before receiving their first dose of the CoronaVac inactivated vaccine. For screening, blood samples were collected and analyzed using SARS-CoV-2 rapid tests and the enzyme-linked immunosorbent assay (ELISA). We observed varying trends in total anti-SARS-CoV-2 antibodies across different variables. Seropositivity among the participants tested was 63.70% (316/496) using the rapid test and 52.82% (262/496) were positive using the ELISA method. The majority of participants identified with the Guarani-Kaiowá ethnic group, with 66.15% (217/328), and other ethnic groups with 58.84% (193/328). The median age of the subjects was 30.5 years, with 79.57% (261/328) being femaleThis research showed the elevated seroprevalence of SARS-CoV-2 antibodies in asymptomatic Brazilians. The findings indicate a high seropositivity rate among the asymptomatic indigenous population of Midwest Brazil. This underscores the overlooked status of these communities and underscores the need for targeted national initiatives that emphasize the protection of vulnerable ethnic groups in the fight against COVID-19.

Effects of Water Deficit on Growth and Productivity in Tomato Crops Irrigated with Water Treated with Very Low-Frequency Electromagnetic Resonance Fields.

The tomato crop is very sensitive to stress conditions. A water deficit is defined as when precipitation is less than the evapotranspiration (ETc) of the crop in a given period, and in this scenario of climate change, it is identified as responsible for global productivity losses. The use of potential technologies for better irrigation management, such as electromagnetically treated water, remains controversial. Thus, the objective of the present work was to investigate the effects of very low-frequency electromagnetic resonance field treatment on water for tomato crops submitted to different irrigation rates. For this, an experiment was carried out under controlled conditions with different types of water: electromagnetically treated water (WTVLF) and untreated water (UNW), as well as four water replacement rates: 40, 60, 80, and 100% ETc. The electromagnetic treatment of the water was carried out using the commercial equipment AQUA4D®. The experiment was carried out in pots with five replications per treatment. Lower activity of SOD, POD, CAT, and APX enzymes was observed in plants irrigated with water treated with very low-frequency electromagnetic resonance fields (WTVLF), indicating less oxidative stress caused by water deficit. Water deficit reduced chlorophyll content, but the effects were less harmful with WTVLF water. The water deficit resulted in less accumulation of dry matter and less productivity in a linear relationship. However, plants irrigated with WTVLF showed increments of about 20% in dry matter accumulation and 20% in fruit production concerning plants irrigated with untreated water, independent of the irrigation rates. We can conclude that irrigation with WTVLF can be a solution to reduce the damage caused by water deficits and increase the productivity of tomato crops.

Fuzzy Modeling Development for Lettuce Plants Irrigated with Magnetically Treated Water.

Due to the worldwide water supply crisis, sustainable strategies are required for a better use of this resource. The use of magnetic water has been shown to have potential for improving irrigation efficacy. However, a lack of modelling methods that correspond to the experimental results and minimize error is observed. This study aimed to estimate the replacement rates of magnetic water provided by irrigation for lettuce production using a mathematical model based on fuzzy logic and to compare multiple polynomial regression analysis and the fuzzy model. A greenhouse study was conducted with lettuce using two types of water, magnetic water (MW) and conventional water (CW), and five irrigation levels (25, 50, 75, 100 and 125%) of crop evapotranspiration. Plant samples for biometric lettuce were taken at 14, 21, 28 and 35 days after transplanting. The data were analyzed via multiple polynomial regression and fuzzy mathematical modeling, followed by an inference of the models and a comparison between the methods. The highest biometric values for lettuce were observed when irrigated with MW during the different phenological stage evaluated. The fuzzy model provided a more exact adjustment when compared to the multiple polynomial regressions.

Determining reference ranges for immunological cells of healthy indigenous individuals from a region in Brazil.

Morais et al. conducted a pioneering study with Brazilian indigenous populations to determine reference values for immunologic cells from healthy adult individuals. The main findings included a higher relative median for T lymphocyte subsets in females than males, and T CD3+, T CD4+, and T CD8+ relative values were statistically different when compared with Brazilian populations from other Brazilian regions. The relative medians of CD3+, CD4+, and CD8+ T cells were significantly higher in women than in men in a healthy indigenous population. Demographic and ethnic diversity of the Brazilian population can be associated with quantitative modifications in the immunologic cells of healthy individuals. OBJECTIVE: The establishment of reference values for a subset of leukocytes is common in clinical practice, and ethnic variations are strongly associated with disease development. In Brazil, indigenous people are vulnerable to infections, and few studies have described the health and disease conditions of this population. This study aimed to provide reference values for immunological cell subsets in indigenous Brazilians living in the state of Mato Grosso do Sul. METHODS: Flow cytometry and 4-color combinations of monoclonal antibodies were used to characterize cells. A total of 115 healthy adults, mostly females (72%), were included in the study. The results are presented as mean and median (2.5%-97.5% percentiles) for T and B lymphocytes, CD4+ T cells, CD8+ T cells, Natural Killer cells, monocytes, and dendritic cells, providing an average immunological profile for the population in question. RESULTS: The relative medians of CD3+, CD4+, and CD8+ T cells were significantly higher in women than in men in a healthy indigenous population. CONCLUSION: To our knowledge, cell reference data from indigenous Brazilians are unknown in the literature. The immune cell results presented in this pioneering study will contribute to the clinical and laboratory evaluation of the Brazilian indigenous population, especially given the important differences when compared with other Brazilian ethnic groups.

Lower-Limb Kinematics and Clinical Outcomes: Correlation Does Not Imply Causality.

Letter to the Editor-in-Chief in response to JOSPT article "Are changes in dynamic knee movement control related to changes in pain or function in people with knee disorders?" by Nunes GS, de Moraes WSLA, de Souza Sampaio V, et al. J Orthop Sports Phys Ther 2023;53(11):1. doi:10.2519/jospt.2023.0203.

_Combining return to sport, psychological readiness, body mass, hamstring strength symmetry, and hamstring/quadriceps ratio increases the risk of a second anterior cruciate ligament injury.

PURPOSE: To investigate the combinations of variables that comprise the biopsychosocial model domains to identify clinical profiles of risk and protection of second anterior cruciate ligament injury. METHODS: One hundred and forty-five patients for return-to-sport testing after anterior cruciate ligament (ACL) reconstruction (ACLR) were contacted, and 97 were deemed eligible. All were evaluated between 6 and 24 months and followed up for 2 years. Participants answered the International Knee Documentation Committee (IKDC) and Anterior Cruciate Ligament-Return to Sport after Injury Scale (ACL-RSI), performed the postural stability assessment using the Biodex Balance System, and assessed muscle strength at 60° and 300°/s on the isokinetic dynamometer. Personal factors (age, gender, body mass index), body structures (graft type and concomitant injuries), and environmental factors (time between surgery and evaluation) were also collected. The participants were asked about the occurrence of a second ACL injury and return to sport after 2 years of follow-up. Classification and regression tree (CART) analysis was used to determine predictors of a second ACL injury. The receiver operating characteristic (ROC) curve was performed to verify the accuracy of the CART analysis, in addition to the sensitivity, specificity, and relative risk (RR) of the model. RESULTS: Of the initial 97 participants, 88 (89.8%) responded to follow-up and 14 (15.9%) had a second ACL injury (11 graft ruptures and three contralateral ACL). CART analysis identified the following variables as predictors of second ACL injury: return to sport, hamstring strength symmetry at 300°/s, ACL-RSI score, hamstrings/quadriceps ratio at 60°/s, and body mass index (BMI). CART correctly identified 9 (64.3%) of the 14 participants who were reinjured and 71 (95.9%) of the 74 participants who were not. The total correct classification was 90.9%. The area under the ROC curve was 0.88 (95% CI 0.72-0.99; p < 0.001), and the model showed a sensitivity of 75% (95% CI 42.8-94.5), specificity of 93.4% (95% CI 85.3-97.8), and RR of 15.9 (95% CI 4.9-51.4; p < 0.0001). CONCLUSION: The combination of hamstring strength symmetry, hamstring/quadriceps ratio (body functions); return to sport (activity and participation); psychological readiness; and BMI (personal factors) could identify three clinical risk profiles for a second ACL injury with good accuracy. LEVEL OF EVIDENCE: IV.

Dose-response effect of lower limb resistance training volume on pain and function of women with patellofemoral pain: A systematic review and meta-regression.

OBJECTIVE: Analyze whether the effects of lower limb resistance training on pain and self-reported function were associated with the exercise volume prescribed for women with patellofemoral pain (PFP). METHODS: A systematic search was undertaken in four databases from inception to May 2023. Eligible trials examined the effects of resistance training programs on pain (visual analogue scale or numerical pain scale) and function (Anterior Knee Pain Scale) in women with PFP. Meta-analysis was undertaken with a random-effects model. The association of resistance training volume-related variables with mean difference effects on pain and function were tested by exploratory univariable meta-regression models. RESULTS: From 1,404 estudies retained for screening after duplicate removals, 16 studies (579 patients) were included. Changes in knee pain were inversely associated with weekly training frequency (ß = 0.5 ± 0.2, P = 0.012). No associations were found between the amount of resistance exercise prescribed per session or per week and effects on pain. Changes in function were associated with the number of sets per week (ß = 0.1 ± 0.1, P = 0.044) and number of sets per session (ß = 0.6 ± 0.2, P < 0.001) over the intervention. Most favorable results were achieved with 17 to 27 sets per session and >45 sets per week. CONCLUSIONS: The amount of prescribed resistance exercise does not seem to be critical for pain reduction in women with PFP. However, our findings support a dose-response effect in terms of improving function.

Consumption of cashew nut induced anxiolytic-like behavior in dyslipidemic rats consuming a high fat diet.

This study aimed to evaluate the effect of cashew nut consumption on anxiety-like behavior in dyslipidemic rats. The groups formed were: Control (CONT), Dyslipidemic (DL) and Dyslipidemic cashew nuts (DLCN). Tests to assess anxiety parameters were performed after the treatment period. Brain fatty acid profiles were analyzed. The animals in the DLCN group showed more rearing than DL, without differing from the CONT and less grooming than either the DL and CONT in the Open Field. In the Elevated Plus Maze, DLCN spent more time on the open arms and in the central area compared to the other groups. As for brain fatty acids, there was a reduction in polyunsaturated fatty acids for the DLCN compared to the other groups. The cashew nut, rich in fatty acids, phenolic and flavonoid compounds, reduced the anxiogenic-like behavior caused by dyslipidemia in rats without altering brain fatty acids.

Construção de procedimento operacional padrão como ferramenta facilitadora de gestão em saúde em uma faculdade de odontologia / Construction of standard operating procedure as a facilitating tool for health management in a school of dentistry / Construcción de procedimiento operativo estándar como herramienta facilitadora para la gestión en salud en una facultad de odontología

O Procedimento Operacional Padrão é uma relevante ferramenta de gestão para otimizar o processo de trabalho dentro da perspectiva da resolutividade definida nos princípios do Sistema Único de Saúde.Objetivo:elaborar um Procedimento Operacional Padrãopara estandardizar o funcionamento do fluxo de materiais do almoxarifado da Faculdade de Odontologia da Universidade Federal do Pará. Metodologia:Estudo descritivo que propôs a criação de uma ferramenta de tecnologia leve dividido em três etapas: aprofundamento teórico, elaboração e refinamento. Resultados:A equipe envolvida no processo de trabalho da unidade de saúde colaborou com a construção do instrumento, favorecendo o estabelecimento de vínculo e assertividade. O Procedimento Operacional Padrãoelaborado constitui subsídio para organização do fluxo com o objetivo de esclarecer, facilitar e padronizar o uso do almoxarifado, promovendo estratégias de uso adequado no manejo dos materiais e a racionalização dos mesmos, com a finalidade de tirar máximo proveito e reduzir custos. Conclusões:A elaboração da ferramenta promoveu aprimoramento do processo de trabalho no manejo dos materiais e equipamentos odontológicos. A participação dos sujeitos envolvidos gerou fortalecimento do vínculo e co-responsabilização (AU).

The Standard Operating Procedure is a relevant management tool to optimizethe work processwithin the perspective of resolution as defined in the Unified Health Systemprinciples.Objective:to elaborate a Standard Operating Procedureto standardize the flow use of materials in the storeroom of the College of Dentistry of the Federal University of Pará.Methodology:Descriptive study which proposed the creation of a soft technology tool divided into three stages: theoretical deepening, elaboration and refinement. Results:The team involved in the health center'swork process collaborated with the development of the instrument, promoting the establishment of bonding and assertiveness. The elaborated Standard Operating Procedureconstitutes subsidy for the organization of the flow with the goal of clarifying, facilitating and standardizing the use of the storeroom, promoting strategies of adequate use in the handling of materials and the rationalization of them, in order to take full advantage and reduce costs. Conclusions:The tool elaborationpromoted the improvement of the work process in the handling of dental materials and equipment. The participation of the health center's team strengthened the bond and co-responsibility (AU).

El Procedimiento Operativo Estándar es una herramienta de gestiónrelevante para optimizar el proceso de trabajo en la perspectiva de resolución definida en los principios del Sistema Único de Salud.Objetivo: desarrollar un Procedimiento Operativo Estándarpara estandarizar el flujo de materiales en el almacén de la Facultad de Odontología de la Universidad Federal de Pará. Metodología: Estudio descriptivo que propuso la creación de una herramienta de tecnología liviana dividida en tres etapas: profundización teórica, elaboración y refinamiento. Resultados: El equipo involucrado en el proceso de trabajo delaunidad de saludcolaboró con la construcción del instrumento, favoreciendo el establecimiento de un vínculoe asertividad. El Procedimiento Operativo Estándarelaboradoconstituyesubsidio para la organización del flujo con el objetivo de aclarar, facilitar y estandarizar el uso del almacén, promover estrategias de uso adecuado en el manejo de los materiales y la racionalización de los mismos, con el fin de aprovechar al máximo y reducir los costos.Conclusiones: La elaboración de la herramienta promovió la mejora del proceso de trabajo en el manejo de materiales y equipos odontológicos. La participación de los sujetos involucrados fortaleció el vínculo y la corresponsabilidad (AU).

Renal cell carcinoma with contralateral adrenal metastasis: Case report.

Renal cell carcinoma is the major cause of kidney malignancy. Its adrenal metastasis is less frequent and is even rarer when it is affected contralaterally or bilaterally. We present the case of a 55-year-old man with diffuse abdominal pain. An irregular mass in the lower third of the left renal cortex and another in the right adrenal gland. Pathology showed it was a renal cell carcinoma with metastasis in the contralateral adrenal gland.