ABSTRACT
Em 1998, a Fundação de Medicina Tropical/Instituto de Medicina Tropical do Amazonas implementou o sistema de vigilância para síndromes febris agudas indiferenciadas, com o propósito de manter vigilância ativa e passiva na Amazônia Ocidental, Brasil, permitindo identificar e diagnosticar os agentes etiológicos causadores de febres agudas. O diagnóstico foi realizado através de estudos sorológicos para a detecção de anticorpos IgM, utilizando-se técnicas de ELISA (Enzyme-linked-immunosorbent assay) e kits ELISA comerciais. Foram analisadas 8.557 amostras de soros de pacientes com suspeita clínica de dengue, 40 por cento dos soros foram ELISA positivos para o vírus da dengue e 26 por cento dos soros foram ELISA positivos para outras doenças exantemáticas virais como rubéola, sarampo, parvovírus, oropouche e mayaro.
Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Dengue , Dengue Virus , Disease Outbreaks , Exanthema , Antibodies, Viral , Brazil , Enzyme-Linked Immunosorbent Assay , Immunoglobulin M , SeasonsABSTRACT
In 1998, the FMT/IMT-AM foundation implemented the surveillance system to diagnose acute undifferentiated febrile syndromes, with the objective of active and passive surveillance in Brazilian western Amazonian rainforest to identify and diagnose the etiologic agents of acute fever. The diagnoses were performed using serological tests to detect IgM antibodies by ELISA (Enzyme-linked-immunosorbent assay) CDC/OPAS or using commercial kits. A total of 8,557 serum samples obtained from patients with clinical suspicion of dengue virus were analyzed. ELISA positive reaction to dengue virus was presented by 40% of the serum samples and 26% of the serum samples had positive ELISA reactions to other exanthematous viral diseases, such as rubella, measles, parvovirus, Oropouche virus and Mayaro virus.
