ABSTRACT
El objetivo fue comparar la rugosidad de un composite nanoparticulado con diferentes técnicas de acabado y pulido. Se obtuvieron 20 discos en conformadores ad-hoc en los que se insertó un composite nanoparticulado en capas de 2mm. Se activó cada incremento durante 30s con una intensidad de 1450 mw/cm2. Todas las probetas se pulieron con la secuencia completa de discos Sof-lex a velocidad media y luego se asignaron a uno de 4 grupos (n=5) tratados de la siguiente manera: G1: sin tratamiento extra; G2: con cepillos Astrobrush y JC-SICRA-G; G3: con pasta de diamantes Diamond Gloss aplicada con fieltro; y pasta de óxido de aluminio Poligloss y fieltro; G4: igual que G3 + cepillos (igual que G2). Las superficies se evaluaron con un perfilómetro óptico tridimensional. Se registró la rugosidad promedio (Ra) en tres puntos de cada espécimen, se consideró la Ra a la media de los tres registros obtenidos. Los datos se analizaron mediante ANOVA de una vía y prueba de Tukey para comparaciones múltiples. Los resultados obtenidos fueron: G1: 132,20nm (26,16); G2: 77,20 nm (20,64); G3: 78,74 nm (10,04); G4: 66,47nm (31,08). Se encontró diferencia estadísticamente significativa entre los grupos (P=0.0017); de las comparaciones múltiples surge que G1 presentó una rugosidad media significativamente mayor que los restantes. G1>G2 (P<0.01), G1>G3 (P<0,05), G1>G4 (P<0,01). En conclusión, el uso de sistemas anexos de pulido permite lograr mayor lisura superficial. El uso de más de un sistema complementario no mejora el resultado (AU)
Subject(s)
Surface Properties , Composite Resins , Dental Polishing/methods , Nanoparticles , Materials Testing , Data Interpretation, Statistical , Analysis of Variance , Imaging, Three-Dimensional , Aluminum OxideABSTRACT
The activities of the crude ethanol extract from Piper cubeba seeds, (-)-cubebin and its semi-synthetic derivatives were evaluated against oral pathogens. The crude ethanol extract was more active against Streptococcus salivarius (MIC value of 80 microg/mL). (-)-Cubebin displayed MIC values ranging from 0.20 mm for Streptococcus mitis to 0.35 mm for Enterococcus faecalis. The natural product (-)-cubebin and its semi-synthetic derivative (-)-hinokinin displayed bacteriostatic activity at all evaluated concentrations, as well as fungicidal activity against Candida albicans at 0.28 mm. The O-benzyl cubebin derivative showed fungistatic and fungicidal effects against C. albicans at 0.28 mm and 0.35 mm, respectively. Also, the other dibenzylbutyrolactone derivatives [(-)-6,6'-dinitrohinokinin and (-)-O-(N,N-dimethylaminoethyl)-cubebin] displayed bacteriostatic and fungistatic effects at the evaluated concentrations. Moreover, the semi-synthetic derivative (-)-6,6'-dinitrohinokinin was the most active compound against all the evaluated microorganisms. Therefore, it may be suggested that the presence of the carbonyl group at C-9 plus the introduction of polar groups in the aromatic rings improve the antimicrobial activity of dibenzylbutyrolactone compounds.
Subject(s)
Anti-Infective Agents/pharmacology , Candida albicans/drug effects , Enterococcus faecalis/drug effects , Lignans/pharmacology , Piper/chemistry , Streptococcus/drug effects , 4-Butyrolactone/analogs & derivatives , 4-Butyrolactone/chemistry , 4-Butyrolactone/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/isolation & purification , Benzodioxoles , Dioxoles/chemistry , Dioxoles/pharmacology , Lignans/chemistry , Lignans/isolation & purification , Microbial Sensitivity Tests , Plant Extracts/chemistry , Plant Extracts/pharmacologyABSTRACT
Heme oxygenase degrades heme to form carbon monoxide. It has been reported that heme oxygenase-derived carbon monoxide may interact with L-glutamate (L-Glu) receptors in the nucleus tractus solitarius (NTS). Integrative studies suggest that heme oxygenase inhibitors raise blood pressure, in part, by inhibiting carbon monoxide formation in the NTS. The currents studies were designed to determine if heme oxygenase inhibitors affect the cardiovascular actions of L-Glu in the NTS. Accordingly, MAP and HR responses to unilateral microinjections of L-Glu (5 nmol/100 nl) into the NTS were measured before and after ipsilateral microinjections of zinc deuteroporphyrin 2,4-bis glycol (ZnDPBG, 4.5 nmol/100 nl) or chromium mesoporphyrin (CrMP, 1.5 nmol/100 nl) in awake rats chronically instrumented with NTS guide cannulaes and arterial catheters. With respect to non-treatment (+36+/-5 mmHg, -107 bpm, n=10), ZnDPBG pre-treatment attenuated the pressor and bradycardic responses to L-Glu (+7+/-3 mmHg, -10+/-6 bpm, P<0.05). CrMP similarly attenuated cardiovascular responses to L-Glu (+47+/-3 mmHg, -68+/-8 bpm vs. +20+/-5 mmHg, -40+/-9 bpm; before vs. after, n=10, P<0.05). Matched series yielded no vehicle- or time-related effects. Our findings suggest that a heme oxygenase product, such as carbon monoxide, may affect NTS glutamatergic neurotransmission to participate in cardiovascular control.
