ABSTRACT
INTRODUCTION: Evaluate the immunohistochemical expression of T-bet and IFN-γ in lower lip (LLSCC) and oral tongue squamous cell carcinoma (OTSCC), verifying the presence of Th1 responses in lesions with different clinical conditions. METHODS AND MATERIALS: Thirty OTSCC and 30 LLSCC were analyzed by immunohistochemistry. T-bet was quantitatively assessed by parenchyma cell and stroma quantification, and IFN-γ was semi-quantitatively analyzed: 1:0-25%; 2:26-50%; 3:51-75%; 4:> 75% immunopositive cells. Histological differentiation degrees were categorized as well differentiated (WD), moderately differentiated (MD), or poorly differentiated (PD). RESULTS: OTSCC presented the highest number of T-bet+, parenchyma (p: 0.006), stroma (p: 0.156), parenchyma/stroma (p: 0.015), with no relationship to histological malignancy grade. IFN-γ higher concentrations in LLSCC were detected in parenchyma, stroma and in parenchyma/stroma (p: 0.000), as well as greater immunoreactivity in WD and MD (p: 0.001). In OTSCC, a positive and statistically significant correlation was observed between T-bet+ in parenchyma and IFN-γ in stroma(r: 0.388; p: 0.034), in addition to a statistically significant positive correlation between T-bet in parenchyma compared to stroma(r: 0.411; p: 0.024) and for IFN-γ in both parenchyma and stroma(r: 0.775; p: 0.000) in LLSCC. Higher T-bet+ was observed in OTSCCs, although higher IFN-γ was detected in LLSCCs. CONCLUSION: Thus, we suggest that, even though LLSCC presented lower T-bet+, the favorable microenvironment in these lesions led to an expressive activation of IFN-γ by T-bet+, considerably acting on Th1 differentiation and in antitumor activity, which, admittedly, present less aggressive behavior, reinforcing once again the important role of this cytokine and its use in strategy to fight cancer.
Subject(s)
Carcinoma, Squamous Cell , Head and Neck Neoplasms , Tongue Neoplasms , Humans , Lip/metabolism , Th1 Cells/metabolism , Squamous Cell Carcinoma of Head and Neck/metabolism , T-Box Domain Proteins/metabolism , Carcinoma, Squamous Cell/metabolism , Head and Neck Neoplasms/metabolism , Tumor MicroenvironmentABSTRACT
OBJECTIVE: The aim was to correlate radiomorphometric indices and biochemical analyses as an auxiliary method in bone evaluation in male and female patients with chronic kidney disease-mineral and bone disorder (CKD-MBD) and controls. MATERIALS AND METHODS: Nine radiomorphometric indices and four biochemical parameters were obtained: mental index (MI), height at the mental foramen, total mandibular height (THM), panoramic mandibular index (PMI), original height of the mandible, alveolar bone resorption, distance from the mental foramen to the alveolar bone crest (MF-ABC), mandibular cortical index (MCI), trabecular bone pattern, parathyroid hormone (PTH), calcium (Ca), phosphorus (P), and Ca × P product (Ca × P). STATISTICAL ANALYSIS: The Mann-Whitney U-test, chi-squared test, and Spearman's correlation were applied at a significance level of 95%. RESULTS: There was a moderate negative and significant correlation between MI, PMI, and PTH in female patients with CKD-MBD as well as between THM, MF-ABC, and Ca × P. The MCI and trabecular bone pattern indicated altered bone quality in male patients. CONCLUSIONS: The radiomorphometric evaluation was an auxiliary, noninvasive method to detect possible alterations in the cortex and mandibular bone trabeculation in male and female patients with CKD-MBD.
ABSTRACT
Antecedentes y métodos: El papel de la respuestainmune en la citopatogenia de la enfermedad periodontalconstituye tema de estudio de diversos investigadores. Elobjetivo de éste trabajo fue investigar a través de inmunohistoquímicala densidad y el patrón de distribución de lacélulas inflamatorias mononucleadas (linfocitos T helper elinfocitos B), utilizando los anticuerpos anti CD4 y antiCD20 en 17 casos de gingivitis crónica y 25 casos deperiodontitis crónica. Resultados: Fue observada inmunopositividadpara las células CD4 en un patrón difuso portodo tejido conectivo de todos los casos de gingivitis crónica.De los 25 casos de periodontitis crónica, 22 (88%)presentaron un patrón de distribución en pequeños focosaislados. Con relación a las células CD20, 16 casos de gingivitiscrónica exhibieron positividad en un patrón focal dedistribución tanto en posición yuxtaepitelial como en áreasdistantes del epitelio (10 casos, 62,5%). En los casos deperiodontitis crónica fue observada marcación positivapara el anticuerpo anti CD20 en 22 casos (88%), predominandoel patrón focal en posición yuxtaepitelial y distantedel epitelio (17 casos, 77,3%). El análisis estadístico nomostró diferencia significativa en la densidad de célulasCD4 y CD20 positivas entre los casos de gingivitis yperiodontitis crónica. Conclusión: Fue concluido que apesar de no ser constatada tal diferencia estadística, hubouna mayor densidad de linfocitos B en ambos grupos,sugiriendo una mayor participación de la respuesta inmunehumoral el los diferentes estadios de la enfermedadperiodontal
Introduction and objectives: the role of immunologicalreaction in periodontal disease has been described byseveral reports. The purpose of present research was toinvestigate by immunohistochemistry the density and distributionprofile utilizing the antibodies anti-CD4 and anti-CD20 in 17 cases of chronic gingivitis and 25 of chronicperiodontitis. Results: It was observed immunopositivityfor CD4 cells in diffuse profile by all connective tissue inall chronic gingivitis cases. Out of the 25 cases of chronicperiodontitis, 22 (88%) presented a focal profile distribution.In relation to the CD 20 cells, 16 cases of chronic gingivitishad shown positivity either juxtaepithelial positionas in distant areas of the epithelia (10 cases, 62.5%). Immunopositivityfor the anti-CD20 was observed in 22 chronicperiontitis cases (88%), predominantly in focal profile injuxtaepithelial and distant position of epithelium (17 cases,77.3%). The statistical analysis demonstrated no differencesin relation to the density of CD4 and CD20 cells betweengingivitis and periodontitis cases studied. Conclusion:It was concluded that, although there was no statisticaldifference, a higher density of lymphocytes B wasobserved in both groups, suggesting the predominant participationof the humoral immunity response in different stagesof the periodontal disease
Subject(s)
Humans , Periodontal Diseases/immunology , CD4 Antigens/analysis , Antigens, CD20/analysis , Gingivitis/immunology , B-Lymphocytes , T-LymphocytesABSTRACT
La displasia ectodérmica hereditaria (DEH) representa un grupo de disturbios caracterizados por aplasia o displasia de estructuras y tejidos derivados del ectoderma. Las estructuras generalmente afectadas incluyen el cabello, piel, uñas, dientes y diversas glándulas. Esta condición representa un disturbio raro y se estima su frecuencia de un caso a cada 10.000 o 100.000 nacimientos, teniendo el dentista un papel importante en su diagnóstico debido a las alteraciones dentales que ocurren. Los autores relatan 3 casos de DEH diagnosticados en una misma familia; además se realiza una breve revisión de la literatura sobre esta condición
