Aggresome-Like Formation Promotes Resistance to Proteotoxicity in Cells from Long-Lived Species.

The capacity of cells to maintain proteostasis declines with age, causing rapid accumulation of damaged proteins and protein aggregates, which plays an important role in age-related disease etiology. While our group and others have identified that proteostasis is enhanced in long-lived species, there are no data on whether this leads to better resistance to proteotoxicity. We compared the sensitivity of cells from long- (naked mole rat [NMR]) and short- (Mouse) lived species to proteotoxicity, by measuring the survival of fibroblasts under polyglutamine (polyQ) toxicity, a well-established model of protein aggregation. Additionally, to evaluate the contribution of proteostatic mechanisms to proteotoxicity resistance, we down-regulated a key protein of each mechanism (autophagy-ATG5; ubiquitin-proteasome-PSMD14; and chaperones-HSP27) in NMR fibroblasts. Furthermore, we analyzed the formation and subcellular localization of inclusions in long- and short-lived species. Here, we show that fibroblasts from long-lived species are more resistant to proteotoxicity than their short-lived counterparts. Surprisingly, this does not occur because the NMR cells have less polyQ82 protein aggregates, but rather they have an enhanced capacity to handle misfolded proteins and form protective perinuclear and aggresome-like inclusions. All three proteostatic mechanisms contribute to this resistance to polyQ toxicity but autophagy has the greatest effect. Overall, our data suggest that the resistance to proteotoxicity observed in long-lived species is not due to a lower level of protein aggregates but rather to enhanced handling of the protein aggregates through the formation of aggresome-like inclusions, a well-recognized protective mechanism against proteotoxicty.

Addressing two-level systems variably coupled to an oscillating field.

We propose a simple method to spectrally resolve an array of identical two-level systems coupled to an inhomogeneous oscillating field. The addressing protocol uses a dressing field with a spatially dependent coupling to the atoms. We validate this scheme experimentally by realizing single-spin addressing of a linear chain of trapped ions that are separated by ~3 µm, dressed by a laser field that is resonant with the micromotion sideband of a narrow optical transition.

Observation of anomalous diffusion and fractional self-similarity in one dimension.

We experimentally study anomalous diffusion of ultracold atoms in a one dimensional polarization optical lattice. The atomic spatial distribution is recorded at different times and its dynamics and shape are analyzed. We find that the width of the cloud exhibits a power-law time dependence with an exponent that depends on the lattice depth. Moreover, the distribution exhibits fractional self-similarity with the same characteristic exponent. The self-similar shape of the distribution is found to be well fitted by a Lévy distribution, but with a characteristic exponent that differs from the temporal one. Numerical simulations suggest that this is due to long trapping times in the lattice and correlations between the atom's velocity and flight duration.

Spectrum of two-level systems with discrete frequency fluctuations.

We study, theoretically and experimentally, an ensemble of two-level systems coupled to an environment which induces random jumps in their resonant frequency. We present a closed-form formula for the spectrum in terms of the resonant frequency distribution and the Poisson rate constant. For a normal distribution the spectrum deviates from a generalized Gumbel function, a well-known result for continuous stochastic Gaussian processes. We perform experiments with optically trapped cold 87Rb atoms and show that the predictions of our theory for a 3D harmonic trap match the measured spectra without fitting parameters.

Process tomography of dynamical decoupling in a dense cold atomic ensemble.

Atomic ensembles have many potential applications in quantum information science. Owing to collective enhancement, working with ensembles at high densities increases the efficiency of quantum operations, but at the same time also increases the collision rate and leads to decoherence. Here we report on experiments with optically trapped 87Rb atoms demonstrating a 20-fold increase of the coherence time when a dynamical decoupling sequence with more than 200 pi pulses is applied. Using quantum process tomography we demonstrate that a dense ensemble with an optical depth of 230 can be used as an atomic memory with coherence times exceeding 3 seconds.

Universal scaling of collisional spectral narrowing in an ensemble of cold atoms.

We study the spectral narrowing induced by collisions in a dense cold atomic ensemble. We report on experiments showing a prolongation of the coherence time of optically trapped 87Rb atoms as the density increases, a phenomenon we call collisional narrowing in analogy to the motional narrowing effect in NMR. We derive an expression for the new dephasing time scale in terms of the collision rate and the inhomogeneous decay time. Remarkably, this time scale universally depends only on the atomic phase space density.