ABSTRACT
The objective of the current work was to explore the possible hypolipidemic and therapeutic impacts of Panax ginseng against cardiac damage in response to ethanol ingestion in male and female rats. 10% ethanol was ingested (2ml/Kg) to both male and female rats daily for fourteen days. The results showed that ingestion of ginseng (150mg/Kg) daily for six weeks to male and female rats intoxicated with ethanol, two weeks after ethanol ingestion, successfully modulated the alterations in the serum lipid profiles, namely, triglycerides (TGs), total cholesterol (TCh), low density lipoprotein (LDL-C) and high density lipoprotein (HDL-C), in male and female rats exposed to ethanol versus untreated intoxicated ones. The plant also pronouncedly attenuated the increases in serum cardiac damage biomarkers, namely lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) compared with ethanol intoxicated untreated rats. In conclusion, this study showed that Panax ginseng has a beneficial impact against ethanol induced hyperlipidemia as a risk element for cardiovascular illness.
Subject(s)
Ethanol/toxicity , Heart Diseases/drug therapy , Hyperlipidemias/drug therapy , Inflammation Mediators/antagonists & inhibitors , Panax , Plant Extracts/therapeutic use , Animals , Heart Diseases/blood , Heart Diseases/chemically induced , Hyperlipidemias/blood , Hyperlipidemias/chemically induced , Inflammation Mediators/blood , Male , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Rats , Rats, WistarABSTRACT
The aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen source, respectively. When the cells of the P. funiculosum were immobilized on different carriers, especially linen fibers, their production ability was successfully maintained for seven successive batches. When the fermentation was carried out using inulin juice prepared from Jerusalem artichoke tubers (in place of pure inulin), inulinase production could be sustained till the second cultivation batch of the P. funiculosum immobilized on linen fibers, yielding 122 U/mL enzyme. Results proved the feasibility of using crude inulin juice as a simple and economic carbon source for the production of inulinase.
