Respuesta estrogénica anabólica del varón ante el estrés físico-emocional / Male anabolic estrogenic response to physical and emotional stress

Este es un análisis prospectivo endocrinológico de la respuesta al ejercicio físico agudo/crónico combinado con el estrés emocional de una población militar de 14 aspirantes a ingresar al Batallón de Fusileros Paracaidistas del Ejército Nacional Mexicano, con patrón de sueño, conducta sexual y dieta controlados. En cada individuo se determinó hormona estimulante del folículo, hormona luteinizante, testosterona, estradiol, prolactina y progesterona plasmáticos. Estas hormonas se relacionaron con elementos del semen. El estudio se dividió en cuatro etapas: fase I: control; fase II: 4.5 horas de ejercicio de calentamiento + 10 km de caminata-trote al día durante seis semanas y primer salto en paracaídas; fase III: 2 horas de ejercicio de calentamiento + 5 km de trote diariamente por seis semanas + salto en paracaídas; y fase IV: similar a la fase III en actividades físicas pero con una duración de doce semanas. De ese análisis la respuesta más inesperada fue una elevación del estradiol sin cambios en la testosterona, hormona luteinizante, hormona estimulante del folículo ni prolactina en la fase II; mientras que en las fases III y IV se detectó incremento de la testosterona a la elevación de la hormona luteinizante y prolactina. Estos cambios hormonales no originaron efectos deletéreos en el semen. El hallazgo de la respuesta estrógenica podría se un índice de utilidad para valorar la respuesta fisiológica/clínica al estrés físico emocional en el varón

Role of testosterone and dihydrotestosterone in spontaneous gynecomastia of adolescents.

To test a possible hormonal mechanism of gynecomastia at puberty, a group of pubertal spontaneous gynecomastia (PSG) and healthy young volunteers (HYV), Tanner's stage II-V, were studied. Peripheral blood samples were obtained for measuring follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), testosterone (T), dihydrotestosterone (DHT), estradiol (E-2) and estrone (E-1). No difference was established in steroids in pituitary hormonal concentration when both groups were compared on a sexual stage-matched control basis, except for T 2 SD in 5/9 subjects of PSG and DHT 2 SD in all of PSG. The T:DHT ratio varied from 5.0 to 15.4 in PSG and from 0.42 to 2.224 in HYV. Whether spontaneous gynecomastia might exist in an enzimatic blockade of 5 alpha-reductase and whether a decrease in the T:DHT ratio might favor the estrogen action for the progression of breast enlargement deserve further analysis.

Role of stress in male fertility.

Specific or non-specific noxious agents induce several disorders in body systems. In the following series some of those agents are associated with disturbances in the pituitary-testis-axis. Testicular responses which represent an adaptive mechanism to the stress may be associated with various syndromes of unexplained infertility.

[Synthetic progestogens versus oral progesterone]. / Progestágenos sintéticos vs progesterona oral.

The antecedents of progesterone natural hormone and related structural groups, chemically and biologically, were described. The synthesis and semi-synthesis of these compounds, is reviewed, and molecular changes, which gave more potency for clinical use, is reviewed. The pharmacology of these groups is manifested, particularly, by the identification of a receptor, its physico-quemical characteristics and relative affinity for tissues as endometrium and myometrium. Furthermore, the progestational quality, the antiestrogenic one, antiandrogenic one and its applications, were evaluated. Finally, several biologic indicators which support the use of oral progesterone and that suggest the re-evaluation of tissular dynamics in reproductive tract, were reviewed.

Sleep deprivation and adaptive hormonal responses of healthy men.

To study the effects of sleep deprivation on the pituitary-testis axis physiology, we measured the circulating levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), testosterone (T), androstanedione (A), dihydrotestosterone (DHT), estradiol (E2), and cortisol (C) in eight healthy men as follows: phase I (control), phase II (24-h restless period), phase III (48-h restless period), and phase IV (24-h recovery period). All samples were taken at 8:00 a.m. There was a significant decrease of T, A, DHT, and E2 in phase II but no decrease in FHS, LH, PRL, or C. In phase III there was no further decrease in any androgen, although E2 decreased along with the increase of PRL. In phase IV E2 and PRL tended to return to baseline values, and the androgens were very similar to the controls. FHS, LH, and C showed no change under the effects of phase III. These data extend the adaptive androgenic response and the association of the role of E2 and PRL to restricted or disturbed sleep in men.

The biological expression of natural progesterone.

Progesterone tablets were orally administered to women with no recent luteal activity. Three hypoestrogenic subjects who received, respectively, a single dose of 150 mg, 300 mg and 300 mg/d/7d, showed absorption and a progressive increase in circulating values in a dose-dependent manner. Five out of nine oligomenorrheic patients experienced withdrawal uterine bleeding with 150 mg/12/5d. Postmenopausal endometrium estrogenised by mestranol (n = 5) showed secretory histological changes after 300 mg/d/7d of oral progesterone. Six women with luteal insufficiency received 300 mg/d after control studies, and this dosage was effective in supplementing the circulating natural hormone so that it reached the level of that of fertile women. However, the endometrial progesterone uptake of these women showed no parallel increase. This study supports the clinical usage of oral progesterone and suggests evaluation of the dynamics of hormone uptake by the reproductive tissues.

Identificacion parcial de estrogenos endogenos en varones. Anticuerpos antiesteroides como causa de variacion. / Partial identification of endogenous estrogens in men. Antisteroidal antibodies as cause of variation

La medicion de estrogenos en sangre en varones sanos (n = 50) se llevo a cabo usando anticuerpos diferentes; se realizo un radioinmunoensayo confiable para analizar estradiol (E-2) y una porcion llamada "fraccion estrogenica" (FrE) con objeto de establecer su aplicacion practica. El anticuerpo SAT-029 tuvo una reaccion cruzada de 100 por ciento para estradiol y de menos de 0.01 para el sulfato de estrona; el RSL-1580 identifico a 100 por ciento de E-2, y menos de 1.0 por ciento de los otros estrogenos, mientras que el RSL-1630 identifico 100 por ciento del E-2 y de estrona y 60 por ciento del sulfato de estrona. Los valores (pg/ml) obtenidos directamente de los extractos etereos se informan como FrE; los resultados fueron: SAT-029, E-2 = 23 + o - (con cromatografia); FrE = 69 + o - 15 (sin cromatografia), FSL-1580, E2 = 39 + o - 6, y RSL-1630, FrE = 85 + o - 18 (sin cromatografia). No se encontro diferencia estadistica cuando se midio el 17 beta-estradiol con dos anticuerpos diferentes (SAT-029 vs RSL-1580) y dos procedimientos diferentes (SAP-029) + la separacion cromatografica, y RSL-1580 sin la separacion cromatografica),la que permitio escoger a este ultimo como metodo rapido. Este trabajo tambien senala alternativas para medir uno o varios estrogenos circulanges

[Hormonal tissue concentration and oral contraceptives: the endometrium]. / Concentración hormonal tisular y los anticonceptivos orales: el endometrio.

PIP: The study investigates hormonal control of the menstrual cycle, adrenal function role, and endouterine menstrual disorders by an analysis of peripheral hormones and of their concentration at endometrial level. The authors also present a new radioimmunoassay method for determining hormone presence in plasma and in the endometrium. 59 ovulatory cycles were considered, and ovarian hormones were found to be 10-20 times higher in tissue than in plasma, but not in women under sequential or combined oral contraceptive treatment. The relation between endometrial estrogens and progesterone with plasma follicle stimulating hormone and luteinizing hormone levels is discussed, together with the significance of tissue hormone concentration.^ieng

Blood and semen androgen values in a young unmarried population.

A radioimmunoassay procedure for testosterone (T) and dihydrotestosterone (DHT) in blood and semen plasma was developed using antisera against both androgens. The method utilized 0.5 ml and 1.0 ml volumes of blood plasma and semen respectively, and satisfies the recognized criteria of reliability. Determinations of T and DHT were performed on 33 healthy male unmarried subjects, aged 21-25 years old, and then compared to the sperm count. The T/DHT blood ratio was 7.8 and the correlation was r = 0.50, P less than 0.01, while the T/DHT semen ratio was 0.42 and the correlation r = 0.55, P less than 0.01. The T/DHT, blood/semen ratio was 10.8. No significant relationship was observed either between blood plasma androgens or semen plasma androgens and sperm concentrations.