A new species and a key to the genus Leiurus Ehrenberg, 1828 (Scorpiones, Buthidae) from Saudi Arabia.

A new species, Leiurushadb Al-Qahtni, Al-Salem, Alqahtani & Badry, sp. nov., is described and illustrated from the Majami al-Hadb Protected Area in the Riyadh Province of Saudi Arabia. The new species is compared with species of Leiurus distributed in Saudi Arabia, especially L.arabicus Lowe, Yagmur & Kovarík, 2014. The integrated results indicate that the population found in Majami al-Hadb represents a distinct species, which is described herein. Moreover, the molecular analysis is conducted on the mitochondrial gene 16S rRNA to compare L.hadb sp. nov. with samples of L.arabicus and L.haenggii from Saudi Arabia. The analysis revealed a genetic divergence ranging from 6.0 to 12%. The combination of molecular evidence and morphological characteristics provides adequate support for recognizing the Majami al-Hadb population as a distinct species. Additionally, an identification key for the genus Leiurus found in Saudi Arabia is also provided.

A new distribution record of Arnold's Gecko, Pristurusminimus (Arnold, 1977) (Squamata, Sphaerodactylidae), in Saudi Arabia.

Reptiles are considered an important element of Saudi Arabia's wildlife to be conserved as a priority. However, the status and distribution of the Kingdom's reptile fauna is not well understood, thus hindering the conservation initiatives. Better understanding of the taxonomy and distribution of the Kingdom's reptile fauna is important for implementing effective conservation measures. Here, we provide the new distribution record of Pristurusminimus (Arnold, 1977; common name, Arnold's Gecko) from southern Saudi Arabia. The species was recorded from the Uruq Bani Ma'arid Protected Area (UBM) of southern Saudi Arabia. Four individuals were captured from different survey sites across the UBM and their species identification was confirmed through a mixture of physical and genetic examination. These results increase the number of species from the Pristurus genus to five for Saudi Arabia and improve the understanding of the Kingdom's reptile fauna and its distribution.

The phylogenetic relationship among two species of genus Nebo (Scorpiones: Diplocentridae) from Saudi Arabia and Middle East.

BACKGROUND: The genus Nebo has been identified as a medically important scorpion species distributed across Arabia and the Middle East. However, its taxonomic status remains unclear. AIM: The molecular phylogeny of two Nebo species from Saudi Arabia and comparative sequences from Palestine is presented based on the mitochondrial cytochrome oxidase subunit I (COI) gene. METHODOLOGY: Scorpion specimens were collected from two different localities, mainly the Southern part of Saudi Arabia. Then, DNA was extracted, amplified using invertebrate universal primers, and sequenced to identify the COI gene. The obtained sequences were analyzed, and phylogenetic trees based on maximum parsimony, neighbor-joining, and Bayesian inference were constructed. RESULTS: The inferred phylogeny indicates the monophyletic status of the family Diplocentridae and its subfamily Nebinae and Diplocentrinae. Also, the phylogenetic analyses support the existence of interspecific and intraspecific variations among/ within Nebo hierichonticus and Nebo yemenensis which may indicate distinct species. CONCLUSION: Further morphological studies with additional specimens from the Arabian Peninsula may reveal possible undiscovered and cryptic species.

Androctonustihamicus sp. nov. from the Mecca Province, Saudi Arabia (Scorpiones, Buthidae).

We describe and illustrate a new scorpion species, Androctonustihamicussp. nov., from the Mecca Province of southwestern Saudi Arabia. The new species is compared to the genus Androctonus Ehrenberg, 1828, which is distributed throughout the Middle East, and especially to A.australis (Linnaeus, 1758). We provide the molecular phylogeny for this species.

Morphological characters and feeding habits of insectivorous Kuhl's pipistrelle bat Pipistrellus kuhlii Cretzschmar, 1830, in different riverine habitats.

This study revises the morphological characters of both sexes of Kuhl's pipistrelle, including external, cranial and dental characters. This study also establishes a baseline for the diet of this insectivorous bat species across a variety of habitats in the Nile Valley, where diets have never before been investigated. Our findings of the stomach contentswere not significantly affected despite the different habitats from which the samples were collected. The three most common insect taxa recovered were Hymenoptera, Lepidoptera and Coleoptera. The percentage volume of insect orders in the diet of Kuhl's bat (63 stomachs) consisted of 29% of Hymenoptera, 9% of Coleoptera, 27% of Lepidoptera, 32% unknown materials and unexpected thing of these insectivorous bats was the presences of 3% fruits. A single order did not dominate the diet of this specie because Hymenoptera was the most fed insect orders by frequency of occurrence 87.3%, Lepidoptera by 74.6%, Coleoptera 36.5% and fruits in 4.8% of samples. Also, they may also be fed by other insect orders, as unknown materials were found in all samples.

Morphometric and meristic diversity of the species Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) in Saudi Arabia.

Intraspecific molecular and morphological variations among geographically isolated populations are useful for understanding the evolutionary processes, which is considered early stage of allopatric speciation. Also, the knowledge of the regional variation of scorpion venom composition is needed to improve antivenom therapeutic management. Androctonus crassicauda (Olivier, 1807) is the most common and medically important species in Arabia and the Middle East. Therefore, this study aimed to investigate the geographic morphological variation among A.crassicaudapopulations, regarding its geographical distribution in unexplored arid regions in Saudi Arabia. Samples were collected and examined morphologically under a dissecting microscope from different four eco-geographical regions. The results of ANOVA and multivariate statistical analyses provide strong evidence of geographical variation. The two populations from OTU3 and OUT4 showed the greatest degree of morphological difference from populations of OUT1 and OUT2. Each OTU3 and OTU4 populations showed significant speciation without overlapping in the two groups, while the remaining overlapped groups comprised two other populations. Several body variables influenced male separation, including carapace posterior width, metasoma 3rd length, and metasoma 2nd length. For females, telson length, metasoma 1st width, and sternite 7th width were highly influential variables. Such variation may suggest the existence of cryptic taxa within A. crassicauda populations in Saudi Arabia. Moreover, metasoma ratios can be used as good indicators in intraspecific variation studies of Scorpions.

Genetic diversity among different species of the genus Leiurus (Scorpiones: Buthidae) in Saudi Arabia and the Middle East.

The molecular phylogenetic relationship among two species of genus Leiurus, from Saudi Arabia with additional comparative sequence data available from Egypt, Oman and Turkey is presented. The molecular phylogeny was performed using maximum parsimony, neighbor joining and bayesian inference. Our results indicate a clear deep splitting between the Western clade, which represented by L. quinuestriatus sequences from Egypt and those from the Eastern clade which encompassing different Leiurus species from Saudi Arabia, Oman and Turkey was shown. Also, the phylogenetic relationship represents additional support for the taxonomic status of Arabian Leiurus species.

Little egret (Egretta garzetta) as a bioindicator of heavy metal contamination from three different localities in Egypt.

This work aimed at using Little Egrets (Egretta garzetta), for the first time in Egypt, as a bioindicator of heavy metal contamination from three different Egyptian land use types (Qillin within Kafr El-Sheikh (S1, agricultural), Toukh within Qalyubia (S2, semi-rural area), and Abu Rawash within Giza (S3, urban)). Concentrations of aluminum (Al), barium (Ba), cobalt (Co), cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn) were analyzed in liver and kidney samples of twenty-six adult Little Egrets collected from the three localities during winter 2018. Moreover, the study calculated the Metal Pollution Index (MPI) to highlight the health of the surrounding environment using birds' internal organs as a mirror. Results revealed that, throughout the three sampling sites, the total metal concentrations in liver samples exceeded that of the kidney, indicating that the liver is the target organ of metal accumulation. The Little Egret's liver was found to be the best-recommended organ to use in future biomonitoring of Cu, Zn, Fe, Mn, and Ni, whereas Al, Co, Cr, Ba, Pb, and Cd can be monitored in the kidney. Cu and Pb were higher in the Little Egret's tissues collected from the agricultural site (S1), whereas Ba, Ni, and Fe were higher in the semi-rural site (S2), followed finally by Cd in the urbanized site (S3). The concentrations of trace elements reported in Little Egrets were within the known background level for water birds. However, alarming concentrations were found for Ni levels in liver (from Qillin and Toukh), as well as Pb and Cr levels in kidney samples (from the three localities). MPI of the eleven studied metals in both liver and kidney of the studied species decreased in the order Qillin (24.36) > Abu Rawash (17.98) > Toukh (3.90). In the three investigated localities, the overall calculated MPI values were higher than one, indicating that the ecosystem is polluted. The study suggested using Little Egrets as a bioindicator of metal contamination.