Constitutional BRCA1 and MGMT Methylation Are Significant Risk Factors for Triple-Negative Breast Cancer and High-Grade Serous Ovarian Cancer in Saudi Women.

Breast cancer (BC) and ovarian cancer (OC) are rapidly increasing in Saudi Arabia. BRCA1 and MGMT epimutations have been linked to a higher risk of these malignancies. The present research investigated the impact of these epimutations on the prevalence of BC and OC among Saudi women. DNA methylation was evaluated using methylation-specific PCR, whereas mRNA expression levels were assessed using qRT-PCR. We evaluated white blood cell (WBC)-BRCA1 methylation in 1958 Saudi women (908 BC patients, 223 OC patients, and 827 controls). MGMT methylation was determined in 1534 of the 1958 women (700 BC patients, 223 OC patients, and 611 controls). BRCA1 methylation was detected in 8.6% of the controls and 11% of the BC patients. This epimutation was linked to 13.8% of the early-onset BC patients (p = 0.003) and 20% of the triple-negative breast cancer (TNBC) patients (p = 0.0001). BRCA1 methylation was also detected in 14% of the OC patients (p = 0.011), 19.4% of patients aged <55 years (p = 0.0007), and 23.4% of high-grade serous ovarian cancer (HGSOC) patients. In contrast, the BRCA1 mutation was detected in 24% of the OC patients, 27.4% of patients aged ≥55 years, and 26.7% of the HGSOC patients. However, MGMT methylation was detected in 10% of the controls and 17.4% of the BC patients (p = 0.0003). This epimutation was linked to 26.4% of the late-onset BC patients (p = 0.0001) and 11% of the TNBC patients. MGMT methylation was also found in 15.2% of the OC patients (p = 0.034) and 19.1% of HGSOC patients (p = 0.054). Furthermore, 36% of the BRCA1-methylated patients and 34.5% of the MGMT-methylated patients had a family history of cancer, including breast and ovarian cancer. Notably, BRCA1 and MGMT mRNA levels were greater in the WBC RNA of the BC patients and cancer-free methylation carriers than in that of the OC patients. Our data indicate that BRCA1 and MGMT epimutations significantly contribute to the development of breast cancer and ovarian cancer in Saudi cancer patients. These blood-based biomarkers could help identify female patients at high risk of developing TNBC and HGSOC at an early age.

Ovarian Volume of Saudi Children and Adolescents in the Southern Region Based on Ultrasound Imaging.

Introduction Sonography is a non-invasive and painless technique used for assessing pelvic anatomy and disorder in children and adolescents. Ovarian growth patterns during infancy and puberty are not completely understood. No consensus exists about the normal measures and morphologic appearance of the ovaries in the southern region of Saudi Arabia. Therefore, this study determined the pattern of ovarian and uterine sizes among Saudi girls and their correlation with age. Methods This study was conducted in the radiology department at Abha Maternity and Children Hospital among girls between 0-13 years. All the participants underwent transabdominal ultrasound, and we measured ovarian volume, uterine length, and endometrial thickness to correlate with chronologic age using the Chi-squared test. Results A Total of 152 females were included in this study. The median age was 72 months, with a minimum of one month and a maximum of 156 months. The Chi-squared test showed a significant correlation between age and ovarian measurement. Age was positively associated with ovarian volume, uterine length, and endometrial thickness (p<0.001).  Conclusion The study concluded that age strongly correlated with the size of the uterus and ovaries, which is crucial in interpreting ultrasound measurements of the pelvic organs correctly.

Application of Artificial Intelligence in Discovery and Development of Anticancer and Antidiabetic Therapeutic Agents.

Spectacular developments in molecular and cellular biology have led to important discoveries in cancer research. Despite cancer is one of the major causes of morbidity and mortality globally, diabetes is one of the most leading sources of group of disorders. Artificial intelligence (AI) has been considered the fourth industrial revolution machine. The most major hurdles in drug discovery and development are the time and expenditures required to sustain the drug research pipeline. Large amounts of data can be explored and generated by AI, which can then be converted into useful knowledge. Because of this, the world's largest drug companies have already begun to use AI in their drug development research. In the present era, AI has a huge amount of potential for the rapid discovery and development of new anticancer drugs. Clinical studies, electronic medical records, high-resolution medical imaging, and genomic assessments are just a few of the tools that could aid drug development. Large data sets are available to researchers in the pharmaceutical and medical fields, which can be analyzed by advanced AI systems. This review looked at how computational biology and AI technologies may be utilized in cancer precision drug development by combining knowledge of cancer medicines, drug resistance, and structural biology. This review also highlighted a realistic assessment of the potential for AI in understanding and managing diabetes.

Development and Analysis of Coding and Tailored Metamaterial for Terahertz Frequency Applications.

This study represents the development and analysis of the types of metamaterial structures for terahertz frequency. Recently, investigations about unique coding metamaterial have become well-known among the scientific community since it can manipulate electromagnetic (EM) waves by utilizing various coding sequences. Therefore, several coding and tailored metamaterial designs were compared and numerically analyzed the performances in this research work. The 1-bit coding metamaterial made up of only "0" and "1" elements by adopting two types of unit cells with 0 and π phase responses were analyzed for the coding metamaterial. Moreover, for the numerical simulation analyses, the well-known Computer Simulation Technology (CST) Microwave Studio software was adopted. This investigation focused on the frequency ranges from 0 to 5 THz. On the other hand, the proposed designs were simulated to find their scattering parameter behavior. The comparison of coding and tailored metamaterial revealed slight differences in the RCS values. The coding metamaterial designs manifested RCS values less than -50 dBm2, while tailored metamaterial designs exhibited less than -60 dBm2. Furthermore, the proposed designs displayed various transmission coefficient result curves for both types of metamaterial. Moreover, the bistatic far-field scattering patterns of both metamaterial designs were presented in this work. In a nutshell, the 1-bit coding metamaterial with a unique sequence can influence the EM waves and realize different functionalities.

The effectiveness of ornamental building materials (tiles) for retrospective thermoluminescence dosimetry.

Like many other decorative building materials, tiles are one of the most precious ornamental ingredients for making a lucrative building image. It not only offers beauty and luxury but also added value by protecting/monitoring ionizing radiation. In this study, the utmost regular use of seven varieties of tile samples has been considered for retrospective thermoluminescence dosimetry. To observe the potentials of tiles for retrospective dosimetry, the major thermoluminescence properties (such as dose-response, energy dependence, self-sensitivity, relative sensitivity, glow curves, repeatability, fading, and also effective atomic number) has been investigated following the annealing - irradiation - readout cycles. Making an allowance for the various TL parameters, White Horse (Mirror Polish) tiles demonstrate suitability to be used as emergency TL dosimeter in 0.5-100 Gy dose array. Comparing the values of Zeff of these numerous tiles' samples (11.6-12.7) with TLD-200 (Zeff = 16.3), it is to be noted that tiles can be used as a suitable material for environmental radiation dosimetry. The thermoluminescent characteristics of tiles in the dose limit from 0.5 Gy to 100 Gy are reported here for the first time.

A New Octagonal Close Ring Resonator Based Dumbbell-Shaped Tuning Fork Perfect Metamaterial Absorber for C- and Ku-Band Applications.

In this paper, a new octagonal close ring resonator (OCRR)-based dumbbell-shaped tuning fork perfect metamaterial absorber for C- and Ku-band applications is presented. This design is a new combination of an octagonal ring close ring resonator with two dumbbell-shaped tuning forks metal strips integrated on epoxy resin dielectric substrate. The proposed perfect metamaterial absorber (PMA) is assessed by finite-integration technique (FIT)-based electromagnetic simulator-Computer simulation technology (CST) software. The anticipated assembly reveals dual resonance frequencies of 6.45 GHz and 14.89 GHz at 99.15% and 99.76% absorption, respectively, for TE incidence. The projected design is augmented through various types of parametric studies, such as design optimization, the effect of the octagonal ring resonator width, and varying the split gap of the double tuning fork. The numerical results are also investigated and verified using the equivalent circuit model, another electromagnetic simulator high frequency structural simulator (HFSS), and different array combinations that showed very negligible disparity. The TE polarization wave is applied to analyze the absorption separately and oblique incidence angle showing polarization insensitivity up to 30° and wide incident angle up to 60°. The presented metamaterial absorber is suitable for satellite communication bands, stealth-coating technology, and defense and security applications.

Effect of COVID-19 on control of T1DM patients in Aseer region of Saudi Arabia.

BACKGROUND: The novel corona virus disease, also known as COVID-19, has emerged as a major health concern globally. Its association with comorbid condition has increased its mortality. Diabetes mellitus (DM) is associated with increased risk of infection in comparison to general population. This risk is higher in type 1 DM that type 2 DM. METHODS: A cross sectional study was done in T1DM patients in whom a structured questionnaire was administered after lockdown. Data regarding social demographical variables, Information regarding sugar levels, psychological effects, changes in weight, exercise habits and other variables were included in the validated, electronic questionnaire. Ethic approval was obtained from the Diabetic center Abha, study duration was from January-2020 to October-2020. RESULTS: Out of 143 total patients (46.9%) were males while 53.1% were females. Mean ± S.D of age was obtained 29.6 ± 1.8. [Figure 1] depicted that 23% of the respondents were effected psychologically. [Figure 2] depicted that 80.4% used insulin as a treatment. [Figure 3] depicted that 8% of the respondents make an emergency visit to the health care centers for high rise in diabetes during lockdown. CONCLUSION: COVID-19 and the lockdown affected the management of T1DM. It resulted in changes in lifestyle, compliance to medication, and psychological impact on the participant.

Toxocara Awareness Among Medical Practitioners in Saudi Arabia.

Human toxocariasis is a zoonotic infection with global and regional impacts. Worldwide it is underestimated and clinically overlooked. Medical practitioners are generally unaware of the extent of the resulting disease spectrum. The objective of the study was to assess knowledge and disease awareness among medical practitioners in Aseer, south-western Saudi Arabia. A questionnaire addressing knowledge about the parasite, its visceral larva migrans and the disease spectrum generated was used to interview participants. The study included 285 participants. In answer to the question what is toxocara, only 27%, answered correctly that it is a nematode, paediatricians being the majority. With regard to years of experience among participants, 56.8% of those who answered correctly had less than 5-year experience, as opposed to 35.4% for those with more than 10-year experience. The cumulative awareness about the disease manifestations and spectrum, i.e. those who knew, was less than 30% across specialties and years of experiences. Lack of awareness regarding Toxocara infection and the disease spectrum it can generate is evident. The consequence for such lack of knowledge within our practising medical community is simply unacceptable as it might translate into misdiagnosis and consequently misguided treatment.

Psychological Impact of Acne Vulgaris on the Young Saudi Population.

Background Acne is a chronic inflammatory skin disease caused by bacterial colonization that damages the pilosebaceous gland on the face and other parts of the body. It is one of the most frequent dermatologic diseases in the young population. Acne vulgaris is a devastating disease, and it has a significant impact on a patient's quality of life, influencing their self-esteem as well as their psychosocial development. This study aimed to explore the psychological symptoms associated with patients with acne, its impact on their quality of life according to their personal characteristics, and to raise the importance of observing and managing psychological symptoms during acne treatment. Methods In this cross-sectional study, data were collected through a paper questionnaire. The questionnaire was composed of two parts, the first includes data about demographics and general health while the second has details about the Dermatology Life Quality Index (DQLI); the Arabic validated version was used for data collection. Data were analyzed using SPSS version 20 (IBM Corp., Armonk, NY). Results According to the impact of acne on respondents' lives, 40.0% have no effect at all, 31.0% have small effects, 19.0% have moderate effects,9.0% have large effects, and 0.7% have extremely large effects. Conclusions Acne is a major problem that affects the quality of life of young patients. Its effect is significantly higher among less educated patients and those with a longer duration of disease.

A-Site Cation Size Effect on Structure and Magnetic Properties of Sm(Eu,Gd)Cr0.2Mn0.2Fe0.2Co0.2Ni0.2O3 High-Entropy Solid Solutions.

Three high-entropy Sm(Eu,Gd)Cr0.2Mn0.2Fe0.2Co0.2Ni0.2O3 perovskite solid solutions were synthesized using the usual ceramic technology. The XRD investigation at room temperature established a single-phase perovskite product. The Rietveld refinement with the FullProf computer program in the frame of the orthorhombic Pnma (No 62) space group was realized. Along with a decrease in the V unit cell volume from ~224.33 Å3 for the Sm-based sample down to ~221.52 Å3 for the Gd-based sample, an opposite tendency was observed for the unit cell parameters as the ordinal number of the rare-earth cation increased. The average grain size was in the range of 5-8 µm. Field magnetization was measured up to 30 kOe at 50 K and 300 K. The law of approach to saturation was used to determine the Ms spontaneous magnetization that nonlinearly increased from ~1.89 emu/g (Sm) up to ~17.49 emu/g (Gd) and from ~0.59 emu/g (Sm) up to ~3.16 emu/g (Gd) at 50 K and 300 K, respectively. The Mr residual magnetization and Hc coercive force were also determined, while the SQR loop squareness, k magnetic crystallographic anisotropy coefficient, and Ha anisotropy field were calculated. Temperature magnetization was measured in a field of 30 kOe. ZFC and FC magnetization curves were fixed in a field of 100 Oe. It was discovered that the Tmo magnetic ordering temperature downward-curve decreased from ~137.98 K (Sm) down to ~133.99 K (Gd). The spin glass state with ferromagnetic nanoinclusions for all the samples was observed. The average and Dmax maximum diameter of ferromagnetic nanoinclusions were calculated and they were in the range of 40-50 nm and 160-180 nm, respectively. The mechanism of magnetic state formation is discussed in terms of the effects of the A-site cation size and B-site poly-substitution on the indirect superexchange interactions.

Insights into Sorption-Mineralization Mechanism for Sustainable Granular Composite of MgO-CaO-Al2O3-SiO2-CO2 Based on Nanosized Adsorption Centers and Its Effect on Aqueous Cu(II) Removal.

Although copper is needed for living organisms at low concentrations, it is one of the pollutants that should be monitored along with other heavy metals. A novel and sustainable composite mineralizing sorbent based on MgO-CaO-Al2O3-SiO2-CO2 with nanosized adsorption centers was synthesized using natural calcium-magnesium carbonates and clay aluminosilicates for copper sorption. An organometallic modifier was added as a temporary binder and a source of inovalent ions participating in the reactions of defect formation and activated sintering. The sorbent-mineralizer samples of specified composition and properties showed irreversible sorption of Cu2+ ions by the ion exchange reactions Ca2+ ↔ Cu2+ and Mg2+ ↔ Cu2+. The topochemical reactions of the ion exchange 2OH- → CO32-, 2OH- → SO42- and CO32- → SO42- occurred at the surface with formation of the mixed calcium-copper carbonates and sulfates structurally connected with aluminosilicate matrix. The reverse migration of ions to the environment is blocked by the subsequent mineralization of the newly formed interconnected aluminosilicate and carbonate structures.

Low-cost commercial borosilicate glass slides for passive radiation dosimetry.

For x- and gamma- irradiations delivering entrance doses from 2- up to 1000 Gy to commercial 1.0 mm thick borosilicate glass microscope slides, study has been made of their thermoluminescence yield. With an effective atomic number of 10.6 (approximating bone equivalence), photon energy dependency is apparent in the low x-ray energy range, with interplay between the photoelectric effect and attenuation. As an example, over the examined dose range, at 120 kVp the photon sensitivity has been found to be some 5× that of 60Co gamma irradiations, also with repeatability to within ~1%. The glow-curves, taking the form of a single prominent broad peak, have been deconvolved yielding at best fit a total of five peaks, the associated activation energies and frequency factors also being obtained. The results indicate borosilicate glass slides to offer promising performance as a low-cost passive radiation dosimeter, with utility for both radiotherapy and industrial applications.

Microscope cover-slip glass for TLD applications.

As a result of the various evolving needs, thermoluminescence dosimetry is constantly under development, with applications intended in environmental and personal radiation monitoring through to the sensing of radiotherapy and radiation processing doses. In radiotherapy dosimetry challenges include small-field profile evaluation, encompassing the fine beams of radiosurgery, evaluations confronting the steep dose gradients of electronic brachytherapy and the high dose rates of FLASH radiotherapy. Current work concerns the thermoluminescent dosimetric properties of commercial low-cost borosilicate glass in the form of thin (sub-mm to a few mm) plates, use being made of microscope cover-slips irradiated using clinical external-beam radiotherapy facilities as well as through use of 60Co gamma irradiators. In using megavoltage photons and MeV electrons, characterization of the dosimetric response has been made for cover-slips of thicknesses up to 4 mm. Reproducibility to within +/5% has been obtained. In particular, for doses up to 10 Gy, the borosilicate cover-slips have been demonstrated to have considerable potential for use in high spatial resolution radiotherapy dosimetry, down to 0.13 mm in present work, with a coefficient of determination in respect of linearity of >0.99 for the thinner cover-slips. Results are also presented for 0.13- and 1.00-mm thick cover slips irradiated to 60Co gamma-ray doses, initially in the range 5- to 25 Gy, subsequently extended to 5 kGy-25 kGy, again providing linear response.

Quality of communication between dentists and dental laboratory technicians for fixed prosthodontics in Riyadh, Saudi Arabia.

The fabrication of a clinically acceptable dental prosthesis requires proper communication between the dentist and the dental technician. Prosthodontic educators have been concerned with this interaction and communication. Fixed prosthodontics laboratories revealed that the technicians are often dissatisfied with the information provided in work authorizations. OBJECTIVE: To evaluate the quality of communication between dentists and laboratory technicians via work authorizations for fixed prosthodontics in both governmental and private dental laboratories in Riyadh area from the technician's perspective. METHODS: A sample of 66 dental laboratories, including all government dental laboratories and a selected number of randomly chosen private dental laboratories from each district of Riyadh (40%), participated in the survey. A questionnaire was developed to include questions related to the following areas of work authorization: clarity and accuracy of instructions, patient information, type of prosthesis, choice of materials, design and shade of the prosthesis and type of porcelain glaze. The questionnaire was answered in a face-to-face interview by technicians who were qualified in fixed prosthetic work. Data were analyzed through parametric tests (T-test and one-way ANOVA) to identify significant values (P < 0.05). RESULTS: This survey showed a lack of communication between dentists and dental laboratories regarding the following: marginal design, pontic design, staining diagram, type of porcelain and glaze needed for the prosthesis. Significant differences were observed between the government and private dental laboratories. There was a greater lack of communication between the dentists and government laboratory technicians in Riyadh. There was no statistically significant difference between private laboratories of different areas in Riyadh city (P < 0.05). CONCLUSION: The quality of communication between dentists and dental technicians in Riyadh can sometimes be inadequate, and governmental laboratories have a lower level of communication.