[Incidence of the detection of erythrocyte antibodies in relation to screening test cells]. / Nachweishäufigkeit erythrozytärer Antikörper (Ak) in Abhängigkeit von den verwendeten Suchtestzellen.

A variety of antibody screening tests are available to detect immunization in patients' sera. However, antibodies can be observed only if corresponding antigens are present on test cells used by an antibody screen. By comparing available test cell kits of different manufacturers we revealed various specificities of antigens present or absent on these cells. Using by an antibody screen Wr(a+), Co(b+) and Kp(a+) test cells additionally to available test cells, we detected in 1000 sera of patients 13 anti-Wr(a) and 1 anti-Co(b) besides 17 antibodies with different specificities.

[3 new antibody detection tests. A comparative study]. / Drei neue Antikörpersuchtests. Eine Vergleichende Untersuchung.

Erythrocyte antibodies are of clinical importance since they may cause decreased red cell survival as the result of hemolytic transfusion reactions, hemolytic disease of the newborn or autoimmune hemolytic anemia. A variety of in vitro antibody screening tests are employed to detect their presence in donor and patient sera. Three new methods now available for pretransfusion testing--two solid phase antiglobulin tests and a gel centrifugation test--are compared in parallel with a conventional tube test.

[Antibody detection in emergency transfusions. A comparison of 3 different methods]. / Antikörpernachweis bei der Notfalltransfusion. Ein Vergleich von 3 unterschiedlichen Methoden.

We compared the manual Polybrene technique to three standard methods (albumin/Coombs, LISS/Coombs, and enzyme/papain) on 113 red cell antibodies. Polybrene identified 8 antibodies of the Rhesus system missed by standard methods, whereas 3 antibodies could only be detected by the standard techniques. Four antibodies were identified only in saline solution at room temperature; 6 were found by use of Polybrene exclusively in the additional Coombs phase. In addition, 61 antibodies were tested by 4 different LISS. No considerable differences in the quality of the various LISS were seen. The manual Polybrene test appears to be suitable for crossmatching and rapid antibody identification in emergency situations.