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1.
Sci Transl Med ; 14(660): eabo6135, 2022 08 31.
Article in English | MEDLINE | ID: mdl-36044599

ABSTRACT

T cell receptor (TCR)-based immunotherapy has emerged as a promising therapeutic approach for the treatment of patients with solid cancers. Identifying peptide-human leukocyte antigen (pHLA) complexes highly presented on tumors and rarely expressed on healthy tissue in combination with high-affinity TCRs that when introduced into T cells can redirect T cells to eliminate tumor but not healthy tissue is a key requirement for safe and efficacious TCR-based therapies. To discover promising shared tumor antigens that could be targeted via TCR-based adoptive T cell therapy, we employed population-scale immunopeptidomics using quantitative mass spectrometry across ~1500 tumor and normal tissue samples. We identified an HLA-A*02:01-restricted pan-cancer epitope within the collagen type VI α-3 (COL6A3) gene that is highly presented on tumor stroma across multiple solid cancers due to a tumor-specific alternative splicing event that rarely occurs outside the tumor microenvironment. T cells expressing natural COL6A3-specific TCRs demonstrated only modest activity against cells presenting high copy numbers of COL6A3 pHLAs. One of these TCRs was affinity-enhanced, enabling transduced T cells to specifically eliminate tumors in vivo that expressed similar copy numbers of pHLAs as primary tumor specimens. The enhanced TCR variants exhibited a favorable safety profile with no detectable off-target reactivity, paving the way to initiate clinical trials using COL6A3-specific TCRs to target an array of solid tumors.


Subject(s)
Immunotherapy, Adoptive , Receptors, Antigen, T-Cell , T-Lymphocytes , Antigens, Neoplasm , Cell Line, Tumor , Cell- and Tissue-Based Therapy , Humans , Immunotherapy, Adoptive/methods , Proteomics , Receptors, Antigen, T-Cell/metabolism , Receptors, Antigen, T-Cell/therapeutic use
2.
Sci Rep ; 11(1): 13333, 2021 06 25.
Article in English | MEDLINE | ID: mdl-34172766

ABSTRACT

Cilia are protrusions of the cell surface and composed of hundreds of proteins many of which are evolutionary and functionally well conserved. In cells assembling motile cilia the expression of numerous ciliary components is under the control of the transcription factor FOXJ1. Here, we analyse the evolutionary conserved FOXJ1 target CFAP161 in Xenopus and mouse. In both species Cfap161 expression correlates with the presence of motile cilia and depends on FOXJ1. Tagged CFAP161 localises to the basal bodies of multiciliated cells of the Xenopus larval epidermis, and in mice CFAP161 protein localises to the axoneme. Surprisingly, disruption of the Cfap161 gene in both species did not lead to motile cilia-related phenotypes, which contrasts with the conserved expression in cells carrying motile cilia and high sequence conservation. In mice mutation of Cfap161 stabilised the mutant mRNA making genetic compensation triggered by mRNA decay unlikely. However, genes related to microtubules and cilia, microtubule motor activity and inner dyneins were dysregulated, which might buffer the Cfap161 mutation.


Subject(s)
Cilia/metabolism , Forkhead Transcription Factors/metabolism , Xenopus Proteins/metabolism , Xenopus laevis/metabolism , Animals , Axoneme/metabolism , Basal Bodies/metabolism , Epidermal Cells/metabolism , Epidermis/metabolism , Female , Male , Mice , Microtubules/metabolism
3.
Development ; 147(21)2020 06 15.
Article in English | MEDLINE | ID: mdl-32376681

ABSTRACT

Cilia are complex cellular protrusions consisting of hundreds of proteins. Defects in ciliary structure and function, many of which have not been characterised molecularly, cause ciliopathies: a heterogeneous group of human syndromes. Here, we report on the FOXJ1 target gene Cfap206, orthologues of which so far have only been studied in Chlamydomonas and Tetrahymena In mouse and Xenopus, Cfap206 was co-expressed with and dependent on Foxj1 CFAP206 protein localised to the basal body and to the axoneme of motile cilia. In Xenopus crispant larvae, the ciliary beat frequency of skin multiciliated cells was enhanced and bead transport across the epidermal mucociliary epithelium was reduced. Likewise, Cfap206 knockout mice revealed ciliary phenotypes. Electron tomography of immotile knockout mouse sperm flagella indicated a role in radial spoke formation reminiscent of FAP206 function in Tetrahymena Male infertility, hydrocephalus and impaired mucociliary clearance of the airways in the absence of laterality defects in Cfap206 mutant mice suggests that Cfap206 may represent a candidate for the subgroup of human primary ciliary dyskinesias caused by radial spoke defects.


Subject(s)
Brain/embryology , Brain/metabolism , Cytoskeletal Proteins/metabolism , Forkhead Transcription Factors/metabolism , Lung/metabolism , Mucociliary Clearance , Sperm Motility , Animals , Axoneme/metabolism , Basal Bodies/metabolism , Cilia/metabolism , Cytoskeletal Proteins/chemistry , Embryonic Development , Epithelial Cells/metabolism , Fluorescence , Hydrocephalus/pathology , Infertility, Male/pathology , Male , Mice, Knockout , Mucus/metabolism , Mutation/genetics , Protein Transport , Spermatozoa/metabolism , Spermatozoa/ultrastructure , Xenopus laevis/embryology , Xenopus laevis/metabolism
4.
Sci Rep ; 8(1): 14678, 2018 10 02.
Article in English | MEDLINE | ID: mdl-30279523

ABSTRACT

The transcription factor FOXJ1 is essential for the formation of motile cilia throughout the animal kingdom. Target genes therefore likely constitute an important part of the motile cilia program. Here, we report on the analysis of one of these targets, Fam183b, in Xenopus and mice. Fam183b encodes a protein with unknown function which is conserved from the green algae Chlamydomonas to humans. Fam183b is expressed in tissues harbouring motile cilia in both mouse and frog embryos. FAM183b protein localises to basal bodies of cilia in mIMCD3 cells and of multiciliated cells of the frog larval epidermis. In addition, FAM183b interacts with NUP93, which also localises to basal bodies. During frog embryogenesis, Fam183b was dispensable for laterality specification and brain development, but required for ciliogenesis and motility of epidermal multiciliated cells and nephrostomes, i.e. the embryonic kidney. Surprisingly, mice homozygous for a null allele did not display any defects indicative of disrupted motile ciliary function. The lack of a cilia phenotype in mouse and the limited requirements in frog contrast with high sequence conservation and the correlation of gene expression with the presence of motile cilia. This finding may be explained through compensatory mechanisms at sites where no defects were observed in our FAM183b-loss-of-function studies.


Subject(s)
Cilia/physiology , Cytoskeletal Proteins/metabolism , Epidermal Cells/physiology , Locomotion , Animals , Basal Bodies/chemistry , Cytoskeletal Proteins/genetics , Forkhead Transcription Factors/metabolism , Gene Knockout Techniques , Mice , Mice, Knockout , Xenopus , Xenopus Proteins/metabolism
5.
Dev Biol ; 423(2): 170-188, 2017 03 15.
Article in English | MEDLINE | ID: mdl-27914912

ABSTRACT

Formation of motile cilia in vertebrate embryos is essential for proper development and tissue function. Key regulators of motile ciliogenesis are the transcription factors FOXJ1 and NOTO, which are conserved throughout vertebrates. Downstream target genes of FOXJ1 have been identified in a variety of species, organs and cultured cell lines; in murine embryonic and foetal tissues, however, FOXJ1 and NOTO effectors have not been comprehensively analysed and our knowledge of the downstream genetic programme driving motile ciliogenesis in the mammalian lung and ventral node is fragmentary. We compared genome-wide expression profiles of undifferentiated E14.5 vs. abundantly ciliated E18.5 micro-dissected airway epithelia as well as Foxj1+ vs. Foxj1-deficient foetal (E16.5) lungs of the mouse using microarray hybridisation. 326 genes deregulated in both screens are candidates for FOXJ1-dependent, ciliogenesis-associated factors at the endogenous onset of motile ciliogenesis in the lung, including 123 genes that have not been linked to ciliogenesis before; 46% of these novel factors lack known homologues outside mammals. Microarray screening of Noto+ vs. Noto null early headfold embryos (E7.75) identified 59 of the lung candidates as NOTO/FOXJ1-dependent factors in the embryonic left-right organiser that carries a different subtype of motile cilia. For several uncharacterised factors from this small overlap - including 1700012B09Rik, 1700026L06Rik and Fam183b - we provide extended experimental evidence for a ciliary function.


Subject(s)
Cilia/metabolism , Fetus/metabolism , Forkhead Transcription Factors/metabolism , Organizers, Embryonic/metabolism , Organogenesis , Respiratory Mucosa/embryology , Animals , Cell Differentiation/genetics , Cell Line , Down-Regulation/genetics , Gene Expression Regulation, Developmental , Gene Ontology , Genetic Association Studies , Genome , Green Fluorescent Proteins/metabolism , Lung/embryology , Lung/metabolism , Mice , Organ Specificity/genetics , Organogenesis/genetics , Reproducibility of Results , Respiratory Mucosa/cytology , Subcellular Fractions/metabolism , Transcriptome/genetics
6.
PLoS One ; 7(10): e47785, 2012.
Article in English | MEDLINE | ID: mdl-23110100

ABSTRACT

The vertebrate organizer and notochord have conserved, essential functions for embryonic development and patterning. The restricted expression of developmental regulators in these tissues is directed by specific cis-regulatory modules (CRMs) whose sequence conservation varies considerably. Some CRMs have been conserved throughout vertebrates and likely represent ancestral regulatory networks, while others have diverged beyond recognition but still function over a wide evolutionary range. Here we identify and characterize a mammalian-specific CRM required for node and notochord specific (NNC) expression of NOTO, a transcription factor essential for node morphogenesis, nodal cilia movement and establishment of laterality in mouse. A 523 bp enhancer region (NOCE) upstream the Noto promoter was necessary and sufficient for NNC expression from the endogenous Noto locus. Three subregions in NOCE together mediated full activity in vivo. Binding sites for known transcription factors in NOCE were functional in vitro but dispensable for NOCE activity in vivo. A FOXA2 site in combination with a novel motif was necessary for NOCE activity in vivo. Strikingly, syntenic regions in non-mammalian vertebrates showed no recognizable sequence similarities. In contrast to its activity in mouse NOCE did not drive NNC expression in transgenic fish. NOCE represents a novel, mammal-specific CRM required for the highly restricted Noto expression in the node and nascent notochord and thus regulates normal node development and function.


Subject(s)
Enhancer Elements, Genetic/physiology , Gene Expression Regulation, Developmental/physiology , Gene Regulatory Networks/physiology , Homeodomain Proteins/metabolism , Notochord/metabolism , Organizers, Embryonic/metabolism , Animals , Embryonic Stem Cells , Enhancer Elements, Genetic/genetics , Gene Expression Regulation, Developmental/genetics , Gene Regulatory Networks/genetics , Homeodomain Proteins/genetics , Mice , Microscopy, Fluorescence , Mutagenesis, Site-Directed , Notochord/embryology , Organizers, Embryonic/embryology , beta-Galactosidase
7.
Development ; 139(7): 1276-84, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22357932

ABSTRACT

The mouse transcription factor Noto is expressed in the node and controls node morphogenesis, formation of nodal cilia and left-right asymmetry. Noto acts upstream of Foxj1, which regulates ciliogenesis in other mouse tissues. However, the significance of Foxj1 for the formation of cilia in the mouse node is unclear; in non-amniote species Foxj1 is required for ciliogenesis in the structures equivalent to the node. Here, we analyzed nodes, nodal cilia and nodal flow in mouse embryos in which we replaced the Noto-coding sequence with that of Foxj1, or in embryos that were deficient for Foxj1. We show that Foxj1 expressed from the Noto locus is functional and restores the formation of structurally normal motile cilia in the absence of Noto. However, Foxj1 is not sufficient for the correct positioning of cilia on the cell surface within the plane of the nodal epithelium, and cannot restore normal node morphology. We also show that Foxj1 is essential for ciliogenesis upstream of Rfx3 in the node. Thus, the function of Foxj1 in vertebrate organs of asymmetry is conserved, and Noto regulates node morphogenesis and the posterior localization of cilia on node cells independently of Foxj1.


Subject(s)
Cilia/physiology , Forkhead Transcription Factors/physiology , Gene Expression Regulation, Developmental , Homeodomain Proteins/physiology , Primitive Streak/physiology , Alleles , Animals , Epithelium/metabolism , Genotype , Mice , Mice, Transgenic , Microscopy, Video/methods , Models, Biological , Models, Genetic , Morphogenesis/genetics , Transcription Factors/metabolism
8.
Proc Natl Acad Sci U S A ; 104(40): 15765-70, 2007 Oct 02.
Article in English | MEDLINE | ID: mdl-17884984

ABSTRACT

The mouse homeobox gene Noto represents the homologue of zebrafish floating head (flh) and is expressed in the organizer node and in the nascent notochord. Previous analyses suggested that Noto is required exclusively for the formation of the caudal part of the notochord. Here, we show that Noto is also essential for node morphogenesis, controlling ciliogenesis in the posterior notochord, and the establishment of laterality, whereas organizer functions in anterior-posterior patterning are apparently not compromised. In mutant embryos, left-right asymmetry of internal organs and expression of laterality markers was randomized. Mutant posterior notochord regions were variable in size and shape, cilia were shortened with highly irregular axonemal microtubuli, and basal bodies were, in part, located abnormally deep in the cytoplasm. The transcription factor Foxj1, which regulates the dynein gene Dnahc11 and is required for the correct anchoring of basal bodies in lung epithelial cells, was down-regulated in mutant nodes. Likewise, the transcription factor Rfx3, which regulates cilia growth, was not expressed in Noto mutants, and various other genes important for cilia function or assembly such as Dnahc5 and Nphp3 were down-regulated. Our results establish Noto as an essential regulator of node morphogenesis and ciliogenesis in the posterior notochord, and suggest Noto acts upstream of Foxj1 and Rfx3.


Subject(s)
Cilia/physiology , Embryonic Development/genetics , Genes, Homeobox , Homeodomain Proteins/genetics , Morphogenesis/genetics , Notochord/physiology , Animals , Animals, Newborn , Functional Laterality/genetics , Gene Expression Regulation, Developmental , In Situ Hybridization , Mice , Mutation
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