Life-history evolution in the orange-tailed skink populations living in different climates.

The life-history traits of ectothermic animals can be influenced by many abiotic factors, including climate. As an ectothermic species, we questioned whether the life-history characteristics of the orange-tailed skink (Eumeces schneiderii) populations differ between two different environments/climates. Our findings showed that the average body size of lizards living in the Mediterranean climate zone was higher than those in the continental climate zone. However, although Mediterranean population had higher mean values regarding average age, there was no discernible difference between the two climate zone populations. When considering all populations collectively, it has been discovered that the species' maximum lifespan is 18 years. Body size notably increased with age in both populations. Through the utilization of the von Bertalanffy equation, the anticipated growth parameters portrayed a highly accurate connection between age and snout-vent length. In conclusion, lizards living in habitats characterized by milder Mediterranean climates were found to have larger body sizes than continental populations, but both populations were comparable in terms of mean age. This difference can be explained by several factors, including activation time, temperature, precipitation, food abundance, and the presence of predators.

Investigation of heavy metals in tissues and habitats of three edible frogs from Türkiye.

The soil, water, and organisms have been contaminated by heavy metals due to human activities and industrialization, which has produced a major environmental problem that has a deleterious effect on human health and food quality. Frogs, one of the good bioindicators for environmental pollution, are also among the alternative essential protein sources for humans. In Türkiye, three of these frogs are edible: Pelophylax ridibundus, Pelophylax bedriagae, and Pelophylax caralitanus, also known as Anatolian water frogs. Hence, to assess the possible health risks that might result from consuming frog legs in addition aquatic habitat of Anatolian water frogs, the water, sediment, and frog tissue samples (muscle and liver) were obtained from 11 different provinces covering all regions of Türkiye and analyzed to determine Cd, Cu, Cr, Zn, Pb, and As concentrations. The results revealed considerable variations in heavy metal concentrations among frog tissues, influenced by the sampling sites and species (ANOVA: p < 0.05). The Estimated Daily Intake (EDI) values, calculated based on the average serving size, were also lower than the Provisional Tolerable Daily Intake (PTDI) levels for adult consumers. Furthermore, the study computed the Target Hazard Quotient (THQ) values for heavy metals, all of which were below the critical value of 1, indicating that consuming the hind leg muscles from these frog species would not pose an adverse health risk for humans.

Prevalence of microplastics in commercially sold soft drinks and human risk assessment.

Due to the increasing global plastic production and use in recent years, the amount of microplastic (MP) accumulating in the environment has also increased. This microplastic pollution potential has been documented mostly in studies of the sea or seafood. The presence of microplastics in terrestrial foods has therefore attracted less attention, despite the potential for future major environmental risks. Some of these researches are related to bottled water, tap water, honey, table salt, milk, and soft drinks. However, the presence of microplastics in soft drinks has not yet been evaluated in the European continent, including Türkiye. Hence, the current study focused on the presence and distribution of microplastics in ten soft drink brands in Türkiye since the water utilized in the bottling process of soft drinks originates from different water supply sources. Using FTIR stereoscopy and stereomicroscope examination, MPs were detected in all of these brands. According to the microplastic contamination factor (MPCF) classification, 80% of the soft drink samples indicated a high level of contamination with microplastics. The study's findings showed that each liter of consumed soft drinks exposes people to about nine microplastic particles, which is a moderate dose when compared to exposure levels in earlier research. It has been determined that bottle-production processes and the substrates used for food production may be the main sources of these microplastics. The chemical components of these microplastic polymers were polyamide (PA), polyethylene terephthalate (PET) and polyethylene (PE), and fibers were the dominant shape. Compared to adults, children were subjected to higher microplastic loads. The study's preliminary data on MP contamination of soft drinks may be useful for further evaluating the risks exposure to microplastics poses to human health.

Microplastic pollution and human risk assessment in Turkish bottled natural and mineral waters.

Microplastics (MPs), which arise from the deterioration of larger plastics that are frequently used in daily life and are smaller than 5 mm in size, are found in many environments and can pose a serious threat to human health. Humans ingest these microplastics unintendedly through drinking water. Although plastic pollution has been extensively investigated in a variety of water sources, research on MP contamination in bottled waters is scarce. Hence, in this study, the presence and distribution of MPs were investigated in 150 samples of bottled natural and mineral water brands in Türkiye. Using FTIR stereoscopy and stereomicroscope analysis, MPs were detected in 43 out of the 50 (86%) of these brands. Among the four types of polymers detected, the most abundant type was polyethylene (33%), polypropylene (31%), polyethylene terephthalate (25%), and polyamid (11%). In comparison to natural waters, mineral waters had larger average-sized particles (63.98 ± 4.06 vs. 104.83 ± 14.28 µm) and higher MP concentrations (4.6 ± 0.5 vs. 12.6 ± 1.6 particles/L). Although the most dominant shape was found as fiber in natural waters, fragments were more prevalent in mineral waters. The estimated daily intakes (EDI) for adults and children were expected to be 0.019 and 0.42 MP/kg/bw/day, respectively, in natural waters while EDI were 0.009 and 0.04 MP/kg/bw/day, respectively, in mineral waters. The results of the study suggest that the EDI and annual intake (EAI) are negligible when compared to other studies. The baseline data on MP contamination of bottled water provided in the present study may be significant and useful for researchers to have a better understanding of microplastic contamination exposure.

Variations in age structure and growth in congeners Lacerta viridis and Lacerta media.

Determining the age of any species allows it to be analyzed from the ontogenetic, demographic, and ecological perspectives. In the present study, we tested the hypothesis that the age structure of congener species (Lacerta media and Lacerta viridis) with the same ecological niche may vary in different areas. In this context, we applied skeletochronology method to reveal various demographic parameters, such as age structure, longevity, age at sexual maturity, growth rate, survival rate, adult life expectancy, and the relationship between age and body size in the green lizard, L. viridis, and the medium lizard, L. media. In L. media and L. viridis, the maximum lifespan was 10 and 8 years, respectively. The mean age and body size of females were significantly greater than those of males in L. media. However, in the examined L. viridis population, no appreciable variation in mean age or body size was found to exist between the sexes. It was estimated that the green lizards reach maturity at the age of 2 or 3 years. However, the L. media reached sexual maturity approximately 1 year later than the congener. The body size markedly increased with age in males for both studied populations. However, in females, body size positively increased with age only in L. media. The approach of skeletochronology that we utilized in this study to assess age structure makes it simple to gather a variety of time-dependent ecological data for such ectothermic species.

Microplastic prevalence in anatolian water frogs (Pelophylax spp.).

Frogs are on the verge of extinction due to various biotic and abiotic stressors. Rivers, lakes, ponds, wetlands, and ditches that make up their habitats are exposed to different anthropogenic pollutants. Today, plastics stand out among these pollutants due to their widespread use; however, the information on microplastic (MP) accumulation in frogs is insufficient. In the present study, adult frog samples were collected from 19 different stations in Türkiye, including marsh frogs (Pelophylax ridibundus) from 18 stations and levantine frogs (Pelophylax bedriagae) from 1 station. MP was found in 147 (82.4%) of the 176 frogs that were analyzed. MP abundance in frogs varied between 0.20 and 18.93 MP individual-1. The characterization of MPs was determined predominantly as follows; PET (70.1%) in polymer type, fiber (92.2%) in shape, navy blue/blue (76.1%) in color, and >90% were smaller than 300 µm. No significant relationship with frog's weight (correlation coefficient = 0.01, P = 0.812) or length (correlation coefficient = 0.06, P = 0.473) and MP abundance was detected. The outcomes of this survey might be a baseline to assess the ecological risks posed by MPs and to guide future experimental research.

Bioaccumulation of Trace Elements and Health Risk Predictions in Edible Tissues of the Marsh Frog.

Certain amphibian species have long served as a valuable protein source for humans, in addition to being good bioindicators for environmental pollutants. Hence, to investigate the consumption outcomes leading to potential health risks, we determined the trace element (TE) levels in the hind leg and liver tissues of marsh frogs (Pelophylax ridibundus), one of the delicacies of several cuisines today. The sediment, water, and frog tissue samples were collected from 15 different locations of NE Turkey and analyzed to determine the arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), vanadium (V), and zinc (Zn) concentrations. The TE concentrations in the sediment, water, and individuals were detected to show significant variations among sampling stations (p < 0.05). Yet, Cd and Pb concentrations of the hind legs cooked and enjoyed in the diets were determined below the European Commission's permitted levels. Furthermore, based on the TEs in edible tissues, consumption of the marsh frog did not appear to pose a risk to humans in terms of provisional tolerable weekly intake (PTWI), target hazard coefficient (THQ), and hazard index (HI).

Determination and molecular analysis of antibiotic resistance in Gram-negative enteric bacteria isolated from Pelophylax sp. in the Eastern Black Sea Region.

The aim of this study was to evaluate the prevalence and types of antimicrobial resistance among Gram-negative enteric bacteria isolated from Pelophylax sp. Fifty-four frogs were collected from six provinces in the Eastern Black Sea Region of Turkey. In the cloacal swab cultures, bacteria from 160 different colonies were identified by biochemical tests, automated systems, and matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The antimicrobial susceptibility tests were performed by the disk diffusion method. The observed drug resistance rate was the highest to ampicillin and cefazolin, while the lowest against ciprofloxacin and tetracycline. In the molecular assays, bla TEM (8 Citrobacter spp.), bla SHV (2 Escherichia coli, 1 Hafnia alvei, and a Serratia liquefaciens), tetA genes (E. coli and Klebsiella spp.) and a class 1 integron without any gene cassette (E. coli) were detected. Among the strains, no plasmid-mediated quinolone resistance [qnrA, qnrB, qnrS, qepA and aac (6 ')-Ib-cr] was found. However, two of three quinolone-resistant Klebsiella strains showed the novel amino acid substitution in the gyrA gene resulting in Ser83Asp and Asp87Glu.The clonality between E. coli isolates was also examined by pulsed-field gel electrophoresis. We consider that multidrug-resistant Gram-negative enteric bacteria in the intestinal microbiota of a cosmopolitan frog species might be a reservoir for antibiotic resistance genes.

Impact of various ecological parameters on the life-history characteristics of Bufotes viridis sitibundus from Turkey.

In this study, we used the skeletochronology method to estimate various growth parameters, such as age structure, minimum and maximum life span, age of sexual maturity, and the relationship between body size and weight of eight different populations of the variable toad, Bufotes viridis sitibundus, in Turkey. Further, we determined the relationship between these parameters and ecologic factors using the partial Mantel test. A significant difference was found among the populations with respect to age, body size, and body weight in both males and females. On average, the maximum life span was recorded as 10 years for males and 11 years for females. In the studied populations, the average age of sexual maturity ranged between 2 and 4 years for both sexes. Sexual dimorphism in terms of snout-vent length (SVL) was not observed between males and females in all the populations. Toads from the higher altitudes tended to be significantly larger, older, and heavier than those from lower altitudes. We concluded that altitude and temperature have an impact on the growth rate, body size, and body mass.