Estudio Cuasi-Experimental: Conocimientos, Actitudes y Prácticas del Autoexamen de Mama en Mujeres Vendedoras de los Mercados de Cuenca ­ Ecuador, 2017 / Quasi-Experimental Study: Improvement of the Knowledge, Attitudes and Practices of Breast Self-Examination in Sellers of the Markets of the City of Cuenca, 2017

INTRODUCCIÓN: El cáncer de mama es un problema de salud pública entre las mujeres; el autoexamen de mama es un método de gran importancia, efectivo, no invasivo y sin costo para la detección oportuna de esta patología. El objetivo que se planteó fue incrementar el nivel de conocimientos, actitudes y prácticas del autoexamen de mama en mujeres vendedoras de los mercados de la ciudad, a través de una intervención educativa participativa basada en su entorno. MÉTODOS: Se realizó un estudio cuasiexperimental, en 115 mujeres vendedoras de los mercados 27 de Febrero y 12 de Abril de la ciudad de Cuenca - Ecuador, durante el periodo de Abril del 2016 a Octubre del 2017. A las cuales se les aplicó un pretest, luego una intervención educativa participativa que constó de 3 sesiones y se aplicó un postest. Se utilizó una encuesta de otro estudio, se adaptó y aplicó para la población seleccionada. Los datos se procesaron en SPSS V 21.0. Para evaluar la diferencia entre el pre y el postest se utilizó la prueba de McNemar para comparar proporciones. RESULTADOS: El rango de edad de las mujeres fue de 18 a 65 años con una media de 40.03 años. El 88.7 % tenían hijos con una mediana de 3 hijos. La media de edad de la menarquia fue 13.15 años y del primer embarazo 20.35 años. Las proporciones de respuestas correctas en el pretest fueron: Conocimientos 54.9 %, actitudes 69.2 %, prácticas 32.2 %; en el postest fueron: Conocimientos 88.4 %; actitudes 83.6 % y prácticas 76.4 %, respectivamente; la diferencia fue significativa en todos los casos. CONCLUSIONES: La intervención educativa mejoró significativamente el conocimiento, las actitudes y las prácticas del autoexamen de mama en la población intervenida.

BACKGROUND: Breast cancer is a public health problem women; Maternal self-examination is a method of great importance, effective, non-invasive and without cost for the timely detection of this pathology. The aim of this approach is to increase the level of knowledge, attitudes and practices of the self-examination of breast in the women sellers of the markets of the city, through a participative educational intervention based on their environment. METHODS: This was a quasi-experimental study was carried out in 115 sellers of the markets February 27 and April 12 in Cuenca - Ecuador, during the period from April 2016 to October 2017. A pretest was applied, then the participative educational intervention was in 3 sessions and a posttest was applied. A survey used in another study was used, adapted and applied for the selected population. The data was processed in SPSS V 21.0. To evaluate the difference between pre and postest, the McNemar test was used to compare proportions. RESULTS: The age range of the women was from 18 to 65 years with an average of 40.03 years. 88.7 % had children with a median of 3 children. The average age of the menarche was 13.15 years and of the first pregnancy of 20.35 years. The proportions of correct answers in the pretest were: knowledge 54.9 %, attitudes 69.2 %, practices 32.2 %; in the subsequent test were: Knowledge 88.4 %; attitudes 83.6 % and practices 76.4 %, respectively; the difference was significant in all cases. CONCLUSIONS: The educational strategy improved significantly the breast self-examination knowledge, attitudes and practices in the intervention population.

Differential modulation of the lactisole 'Sweet Water Taste' by sweeteners.

Pre-exposure to taste stimuli and certain chemicals can cause water to have a taste. Here we studied further the 'sweet water taste' (SWT) perceived after exposure to the sweet taste inhibitor lactisole. Experiment 1 investigated an incidental observation that presenting lactisole in mixture with sucrose reduced the intensity of the SWT. The results confirmed this observation and also showed that rinsing with sucrose after lactisole could completely eliminate the SWT. The generalizability of these findings was investigated in experiment 2 by presenting 5 additional sweeteners before, during, or after exposure to lactisole. The results found with sucrose were replicated with fructose and cyclamate, but the 3 other sweeteners were less effective suppressors of the SWT, and the 2 sweeteners having the highest potency initially enhanced it. A third experiment investigated these interactions on the tongue tip and found that the lactisole SWT was perceived only when water was actively flowed across the tongue. The same experiment yielded evidence against the possibility that suppression of the SWT following exposure to sweeteners is an aftereffect of receptor activation while providing additional support for a role of sweetener potency. Collectively these results provide new evidence that complex inhibitory and excitatory interactions occur between lactisole and agonists of the sweet taste receptor TAS1R2-TAS1R3. Receptor mechanisms that may be responsible for these interactions are discussed in the context of the current model of the SWT and the possible contribution of allosteric modulation.

Retrospective Evaluation of Nail Trimming as a Conservative Treatment for Ulcerative Dermatitis in Laboratory Mice.

Ulcerative dermatitis (UD) is an idiopathic disease that affects C57BL/6 mice and those having a B6 background. The hallmark of UD is pruritus, which leads to self-mutilation and epidermal ulceration typically in the intrascapular region. Although several treatments for UD have been published, some involve the use of pharmacologic agents that might confound research results. In this retrospective study, we evaluated nail trimming to determine whether this conservative treatment approach improved the resolution rate of UD at our institution compared with that of untreated mice or those that received oral or topical antibiotics. Our findings show that the incidence of resolution of UD was significantly greater and that the time to resolution was shorter in mice treated with nail trimming compared with other groups. These findings support the use of nail trimming as an effective conservative treatment option for UD in B6 mice.

The Effect of Temperature on Umami Taste.

The effect of temperature on umami taste has not been previously studied in humans. Reported here are 3 experiments in which umami taste was measured for monopotassium glutamate (MPG) and monosodium glutamate (MSG) at solution temperatures between 10 and 37 °C. Experiment 1 showed that for subjects sensitive to MPG on the tongue tip, 1) cooling reduced umami intensity whether sampled with the tongue tip or in the whole mouth, but 2) had no effect on the rate of umami adaptation on the tongue tip. Experiment 2 showed that temperature had similar effects on the umami taste of MSG and MPG on the tongue tip but not in the whole mouth, and that contrary to umami taste, cooling to 10 °C increased rather than decreased the salty taste of both stimuli. Experiment 3 was designed to investigate the contribution of the hT1R1-hT1R3 glutamate receptor to the cooling effect on umami taste by using the T1R3 inhibitor lactisole. However, lactisole failed to block the umami taste of MPG at any temperature, which supports prior evidence that lactisole does not block umami taste for all ligands of the hT1R1-hT1R3 receptor. We conclude that temperature can affect sensitivity to the umami and salty tastes of glutamates, but in opposite directions, and that the magnitude of these effects can vary across stimuli and modes of tasting (i.e., whole mouth vs. tongue tip exposures).

Pathogenicity of Helicobacter ganmani in mice susceptible and resistant to infection with H. hepaticus.

Helicobacter spp. are some of the most prevalent bacterial contaminants of laboratory mice. Although abundant data regarding the diseases associated with H. hepaticus infection are available, little is known about the pathogenicity of H. ganmani, which was first isolated in 2001 from the intestines of laboratory mice. The objective of this study was to evaluate the host response to H. ganmani colonization in H. hepaticus disease-resistant C57BL/6 and disease-susceptible A/J and IL10-deficient mice. Mice were inoculated with H. ganmani, H. hepaticus, or Brucella broth. Cecal lesion scores, cecal gene expression, and Helicobacter load were measured at 4 and 90 d after inoculation. At both time points, mice inoculated with H. ganmani had similar or significantly more copies of cecum-associated Helicobacter DNA than did mice inoculated with H. hepaticus. When compared with those of sham-inoculated control mice, cecal lesion scores at 4 and 90 d after inoculation were not significantly greater in H. ganmani-inoculated A/J, C57BL/6, or IL10-deficient mice. Analysis of cecal gene expression demonstrated that H. ganmani infection failed to cause significant elevations of IFNγ in A/J, C57BL/6, or IL10-deficient mice. However, in IL10-deficient mice, H. ganmani infection was associated with a significant increase in the expression of the proinflammatory cytokine IL12/23p40. Although H. ganmani infection in this study failed to induce the typhlitis that is the hallmark of H. hepaticus infection, infection with H. ganmani was associated with alterations in inflammatory cytokines in IL10-deficient mice.

The laboratory animal veterinarian: more than just a mouse doctor.

Use of animals in research is strictly regulated by federal laws that define how the animals can be humanely housed, studied, and sold. Veterinary care for these animals is also required. Laboratory animal veterinarians serve as a unique bridge between the humane use of laboratory animals and the advancement of scientific and medical knowledge.

An examination of advanced cancer caregivers' support provided by staff interventions at hospices in Argentina.

The aim of the study was to describe the type of intervention provided by hospice staff in order to address the pragmatic, psycho-social, and spiritual needs of home-caregivers for patients in the last stage of cancer. The qualitative inquiry was carried out in real life contexts. The explicit demands that caregivers (n = 40) identified in the first interviews were: (1) helping to organize the care of the patient at home; (2) unspecific demands, with unclear or unrealistic purposes (e.g., curative treatment or a miracle expected to occur); (3) specific resources (such as formal caregivers to replace them), and (4) a place to leave the patient either for a temporary period (a respite for the family) or in a permanent way. The main issues discussed were the delays in the patients' referral to the hospice and the lack of time for long-term interventions; explicit focus is placed on the care by addressing the spiritual and emotional needs of caregivers, unlike in hospital settings where professionals avoid discussions of spiritual needs due to a lack of time, inadequate training and poor understanding of spirituality; hospices' interventions are based upon an ethos similar to the movement's original Christian spirit with emphasis placed on qualities of care such as love, charity, and compassion besides expertise and end-of-life competence, all while tolerating a sense of abandonment by health and social security systems following the patient's referral.

Persistent infection of rhesus monkeys with 'Helicobacter macacae' and its isolation from an animal with intestinal adenocarcinoma.

A novel helicobacter, 'Helicobacter macacae', was previously isolated from a colony of rhesus and cynomolgus monkeys in which diarrhoea from chronic idiopathic colitis was enzootic. A survey performed in a second colony of rhesus monkeys without a history of chronic diarrhoea determined that 57 % were faecal-culture positive for Helicobacter species. Ten years after the survey, one of the animals from which 'H. macacae' had been isolated, a 23-year-old, intact male rhesus monkey (Macaca mulatta), presented with partial inappetence and progressive weight loss. Subsequent evaluation of the monkey revealed anaemia, hypoproteinaemia, hypoalbuminaemia and a palpable abdominal mass. Contrast radiography suggested partial intestinal obstruction. The animal was euthanized and a diagnosis was made of intestinal adenocarcinoma of the ileocaecocolic junction with metastasis to regional lymph nodes and liver. Microaerobic culture of caecal tissue yielded a helicobacter organism identified as 'H. macacae' by 16S rRNA gene sequencing - the same species of bacteria isolated 10 years previously. The liver, small intestine and colon were also positive by PCR for Helicobacter species. Intestinal adenocarcinoma is the most common malignancy of aged macaques. Faeces or caecal tissue from five out of five monkeys that remained from the original cohort and that were colonized with 'H. macacae' in the initial survey were positive for the organism. The apparent persistence of 'H. macacae' in these animals, the isolation of the bacterium from animals with colitis and the recognition of the importance of inflammation in carcinogenesis raise the possibility of an aetiological role in the genesis of intestinal adenocarcinoma in aged rhesus monkeys.

Efecto de la ranitidina en el colesterol total de Rattus rattus var. albinus / Effect of ranitidine in the total cholesterol in Rattus rattus var. albinus

¿Cuál es el efecto de la ranitidina en el colesterol total promedio de Rattus var. albinus hembras? Objetivo: Determinar el efecto de la ranitidina en el colesterol total promedio en Rattus rattus var. albinus hembras. Materiales y Métodos: Se realizó un estudio experimental prospectivo y de doble ciego. Se utilizó 60 rattus rattus albinus hembras divididos en grupo control y experimental. El colesterol total se determinó mediante la prueba enzimática de colesterol éster hidrolasa y colesterol oxidasa. El colesterol total se determinó mediante la prueba enzimática de colesterol éster hidrolasa y colesterol oxilasa. Para el análisis estadístico se utilizó mediante de tendencia central y prueba t-student para medidas pareadas con una confianza de 95 por ciento. Resultados: Se encontró que el colesterol total promedio basal en el grupo control fue de 74,3+16,9 mg/dl y que luego de 21 días fue de 65,4+21,4 mg7dl (p>0.05), mientras que en el grupo experimental de colesterol total promedio basal fue de de 50,9+18,2 mg/dl y luego de 21 días de administración de ranitidina fue de 111.9+48,4 mg/dl (p<0.001). Conclusiones: El valor de colesterol total promedio en Rattus rattus vas. albinus hembras aumenta tras la administración de ranitidina.

What is the defect of ranitidine om the total cholesterol of frenale Rattus var, albinus?. Objective: To determine the effect of ranitidine in the average total cholesterol in Rattus rattus var. albinus females. Materials and Methods: We performed a prospective pilot study and double blind. Rattus rattus was used albinus 60 females divided into control and experimental group. Total cholesterol was determined by an enzymatic test cholesterol ester hydrolase and cholesterol oxidase. Total cholesterol was determined by an enzymatic test cholesterol and cholesterol ester hydrolase oxilasa. The statistical analysis was used by central tendency and t-student test for paired measures with a confidence of 95 percent. Results: We found that the mean baseline total cholesterol in the control group was 74.3 +16.9 mg / dl and after 21 days was 65.4 +21.4 mg7dl (p> 0.05), while in the experimental group mean total cholesterol from baseline was 50.9 +18.2 mg / dl and 21 days after administration of ranitidine was 111.9 +48.4 mg / dl (p <0.001). Conclusions: The average total cholesterol value in Rattus rattus you. albinus females increases after administration of ranitidine.