ABSTRACT
Se determinó la seroprevalencia de cisticercosis porcina en caseríos de la zona de Tumbes, Perú. Se evaluó el 97% (1872/1927) del total de cerdos mayores a 7 meses de edad, provenientes de 17 caseríos rurales de la provincia de Tumbes, departamento de Tumbes, mediante la prueba de Electroinmunotransferencia. Además, se evaluó las variables caserío de procedencia, edad y sexo para determinar si existía asociación respecto a la presentación de la enfermedad, mediante la prueba de Chi Cuadrado y el análisis de Regresión Logística. La seroprevalencia corregida fue de 45 ± 2.3%. Los caseríos que presentaron las seroprevalencias más altas fueron La Capitana, Carretas y Rica Playa (70 ± 7.8, 69 ± 12.3 y 64 ± 6.8%, respectivamente). Los seroprevalencia en cerdos mayores de 12 meses fue de 49 ± 3.1% en tanto que en cerdos de 8 a 12 meses fue de 40 ± 3.3% (p<0.05). No se encontró diferencias estadísticas por efecto del sexo. Los resultados demuestran que la cisticercosis porcina constituye un serio problema de salud pública para los caseríos estudiados.
The porcine cysticercosis seroprevalence was determined in rural villages of Tumbes, Peru. Samples from 97% (1872/1927) of pigs, older than 7 months of age, from 17 rural villages of Tumbes province, Peru, were analyzed by the Electroimmunotransfer test. Also, village of origin, age, and sex were assessed to determine whether there was association with regard to the presentation of the disease by the Chi square test and logistic regression analysis. Seroprevalence was 45 ± 2.3%. Villages with higher seroprevalences were La Capitana, Carretas, and Rica Playa (70 ± 7.8, 69 ± 12.3, and 64 ± 6.8% respectively). Seroprevalence in pigs older than 12 months of age was 49 ± 3.1%, while in pigs 8 to 12 months of age was 40 ± 3.3% (p<0.05). There was no statistical difference due to sex. The results show that porcine cysticercosis is a serious public health problem for the villages surveyed.
Subject(s)
Animals , Cysticercosis , Seroepidemiologic Studies , PeruABSTRACT
El presente estudio se llevó a cabo con 23 venados cola blanca ( Odocoileus virginianus), de ambos sexos, y criados en cautiverio en la provincia de Lima, con el objetivo de determinar el perfil bioquímico sanguíneo hepático a través de los valores séricos de Bilirrubina Total y Directa, Proteínas Totales, Albúmina, Alanino Amino Transferasa (ALT), Aspartato Amino Transferasa (AST), Fosfatasa Alcalina (FA), y Gamma Glutamil Transferasa (GGT). Los animales (7 machos y 16 hembras), pertenecientes a tres zoológicos o zoocriaderos, fueron anestesiados con clorhidrato de ketamina o con la combinación de clorhidrato de ketamina y clorhidrato de xilacina. Se extrajo 7 ml de sangre por punción de la vena safena en tubos estériles sin anticoagulante, y los sueros fueron analizados con kits comerciales. Los valores encontrados (media ± DE) fueron para Bilirrubina Total: 0.6 ± 0.3 mg/dl; Bilirrubina Directa: 0.08 ± 0.06 mg/dl; Proteínas Totales: 6.6 ± 0.7 g/dl; Albúmina: 3.6 ± 0.5 g/dl; ALT: 26.0 ± 9.7 UI/l; AST: 87.6 ± 22.9 UI/l; FA: 73.9 ± 33.8 UI/l; y GGT: 42.5 ± 12.6 UI/l. No hubo diferencia estadística entre sexos con excepción de BT y albúmina (p<0.05).
This study was carried out with 23 adult white-tailed deer (Odocoileus virginianus) of both sexes, kept in captivity in the province of Lima to establish reference liver biochemistry profile values (Total and direct bilirubin, total protein, albumin, alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (AF), and gamma glutamyl transferase (GGT). The animals (7 males and 16 females) were from three local zoos and were anesthesyzed with ketamine hydrochloride or with a combination of ketamine hydrochloride and xylazine hydrochloride. Blood samples (7ml) were collected from the saphenous vein in sterile tubes and serum was analyzed using commercial kits. The values (mean ± SD) were Total Bilirubin: 0.6 ± 0.3 mg/dl; Direct Bilirubin: 0.08 ± 0.06 mg/dl; Total Protein: 6.6 ± 0.7 g/dl; Albumin: 3.6 ± 0.5 g/dl; ALT: 26.0 ± 9.7 UI/l; AST: 87.6 ± 22.9 UI/l; AF: 73.9 ± 33.8 UI/l, and GGT: 42.5 ± 12.6 UI/l. There were no statistical differences due to sex except for total bilirubin and albumin (p<0.05).
Subject(s)
Animals , Biochemistry , Deer , Deer/blood , Liver , Reference ValuesABSTRACT
Se determinó los niveles séricos de las enzimas asparto aminotransferasa (AST), creatina fosfokinasa (CK), isoenzima creatina fosfokinasa-MB (CK-MB) y lactato deshidrogenasa (LDH) en perros adultos con enfermedad cardiovascular (ECV) y aparentemente sanos, para evaluar su uso como predictor de daño miocárdico en insuficiencia cardíaca. Se evaluó a 25 animales sin distinción de sexo y raza, los cuales se dividieron en 2 grupos: 10 animales aparentemente sanos (G1) y 15 animales con enfermedad cardiovascular (G2). Se colectó sangre de la vena cefálica y los sueros resultantes se analizaron por espectrofotometría. Los niveles séricos de la isoenzima CK-MB fueron los únicos que tuvieron diferencia estadística (p<0.05) a favor de los animales con ECV. Se concluye que la CK-MB puede servir para predecir el daño miocárdico progresivo en la insuficiencia cardíaca canina.
Serum levels of the asparte amino transferase (AST), creatine kinase (CK), the MB fraction of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) enzymes were measured in adult dogs with cardiovascular disease to evaluate their potential use as predictors of myocardial damage in heart failure. Twenty five animals, irrespective of sex and breed, were studied: 10 of them were apparently healthy (G1) and the other 15 suffered of cardiovascular illnesses (G2). Blood samples were collected and the resulting sera was examined by spectrophotometry. The results indicated that only levels of CK-MB were statistically different (p<0.05) between the two groups. It is concluded that CK-MB can be used to predict progressive myocardial damage in dog´s heart failure.
Subject(s)
Dogs , Aspartate Aminotransferases , Creatine Kinase , Dogs , Cardiovascular Diseases , Lactate DehydrogenasesABSTRACT
The objective of this research was to establish reference serum values for urea and creatinine in the captive white-lipped peccary (Tayassu pecari). Serum samples from 44 adult animals (23 males and 21 females) from a captive colony in the Parque de las Leyendas Zoo (Lima, Peru) were collected after chemical inmobilization. Urea and creatinine were analyzed using colorimetric assays, and values were 27.4 ± 2.7 mg/dl and 2.8 ± 0.2 mg/dl respectively. No significant differences between sexes were found. Urea value was greater than those obtained in other studies probably due to the high protein content of the zoo diet.
Subject(s)
Animals , Biomarkers/analysis , Creatinine/adverse effects , Swine , Urea/adverse effects , PeruABSTRACT
The objective of this study was to establish reference serum values for alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin (total and direct), alkaline phosphatase, total proteins and albumin in captive South American coati (Nasua nasua). A total of 19 coatis (11 males and 8 females) from two zoo parks in the area of Lima, Peru were blood sampled. The coatis were chemically restrained using ketamine clorhidrate (10 mg/kg body weight) and xilacine clorhidrate (1 mg/kg body weight) via IM. Blood samples (4 ml) were collected through femoral vein puncture using vacutainer tubes without anticoagulant. The blood chemistry values obtained were ALT: 94.0 ± 48.5 UI/L, AST: 124.7 ± 49.4 UI/L, total bilirubin: 0.72 ± 0.55 mg/dl, direct bilirubin: 0.19 ± 0.21 mg/dl, indirect bilirubin: 0.52 ± 0.52 mg/dl, alkaline phosphatase: 46.8 ± 26.4 UI/L, total proteins: 8.0 ± 1.1 g/dl, and albumin: 3.9 ± 0.5 g/dl.
Subject(s)
Animals , Biochemistry/instrumentation , Liver , Procyonidae , Serum/chemistryABSTRACT
Cistozoitos obtenidos a partir de macroquistes de Sarcocystis aucheniae presentes en carne de alpacas con sarcocistiosis fueron inoculados en cultivo primario de células de cornete nasal de feto de alpaca (CNA) y en líneas de células establecidas de riñón de bovino (BHK) y de mono (VERO). Los cistozoitos ingresaron a todas las células, transformándose en grandes y pequeños esquizontes multinucleares o en estadios como quistes, produciendo gran cantidad de merozoitos, todos de posición perinuclear. Cultivos continuos a partir del inóculo original de cistozoitos fueron mantenidos por más de 150 días por subcultivo de cistozoitos sobre nuevos cultivos de células CNA, BHK y VERO. Durante este tiempo, se mantuvo la capacidad de producir ambos tipos de esquizontes. Se confirmó la reproducción de los cistozoitos por tinción Giemsa y mediante inmunofluorescencia e inmunoperoxidasa, ambas pruebas utilizando antisuero anti S. aucheniae producido en conejos.
Cystozoites obtained from macrocysts of Sarcocystis aucheniae, collected from alpaca meat infected with sarcocystiosis, were inoculated onto primary monolayer cultures of alpaca turbinate (AT) and secondary monolayer of bovine kidney (BHK) and monkey kidney (VERO) cells. Sporozoites entered the cells, formed large and small multinucleate schizonts, and produced large numbers of cystozoites of perinuclear position. Continuous cultivation from the original cystozoite inoculum was maintained for more than 150 days by inoculating cystozoites onto new cultures of AT, BHK and VERO cells. During this time, the capacity to produce both types of schizonts was preserved. The reproduction of cystozoites of S. aucheniae in tissue culture was confirmed using the Giemsa stain and the fluorescence microscopy and immunoperoxidase test, both of them, using antibodies anti S. aucheniae produced in rabbit.
Subject(s)
Animals , Camelids, New World/parasitology , Cells , Sarcocystis , Immunoenzyme TechniquesABSTRACT
Se determinó el grado de concordancia entre el examen hematológico y la prueba de ELISA directa en el diagnóstico de ehrlichiosis canina. Se emplearon 77 muestras de sangre de perros con signos clínicos compatibles a ehrlichiosis canina y 20 controles clínicamente normales que fueron obtenidos en la Clínica de Animales Menores de la Universidad Nacional Mayor de San Marcos. Se encontró un 84.7 ± 11.0 por ciento de grado de concordancia mediante la prueba de Kappa. Así mismo, se determinó que la trombocitopenia, leucopenia, anemia y el antecedente de contacto con garrapatas tuvieron una relación significativa con la presencia de la enfermedad (p menor que 0.05). Se concluye que el examen hematológico es muy importante en el diagnóstico de la ehrlichiosis canina y que el ELISA directo es una excelente prueba confirmatoria.
The present study evaluated the level of agreement between the hematology test and the direct ELISA assay for the diagnosis of canine ehrlichiosis. It was used 77 samples from dogs with clinical signs compatible with canine ehrlichiosis and 20 control samples from the Hospital of Small Animals of the Veterinary Medicine Faculty, San Marcos National University. The results showed 84.7 ± 11.0 per cent of concordance by the Kappa test. Likewise, the thrombocytopenia, leukopenia, anemia and history of tick infestation were significantly related (p minor that 0.05) to clinical evidence of the disease. The results revealed that the hematology test was important in the diagnosis of canine ehrlichiosis and that the direct ELISA was an excellent confirmatory test.
Subject(s)
Animals , Ehrlichia canis , Enzyme-Linked Immunosorbent Assay , Hematologic Tests , ThrombocytopeniaABSTRACT
The objective of the study was to determine the presence of antibodies against Actinobacillus suis in pigs from technical breeding farms located in the northern, central and southern part of Lima. Blood samples of apparently healthy Actinobacillus suis-non-vaccinated pigs of 17-20 weeks of age were obtained from the local slaughterhouse (n=474). Serological evaluation was performed through a plate agglutination test with a specific antigen for Actinobaccillus suis, using three dilutions (1/40, 1/80 and 1/160). The volume of sera was 0.08, 0.04 and 0.01 ml, whereas the amount of antigen was kept constant (0.03 ml). Only those samples that agglutinated in all three dilutions were considered as positive reactors. The results indicated that 142 out of 474 sera tested showed antibodies against A. suis obtaining a prevalence of 30% with higher frequency of positives in the southern and northern part of Lima.
El presente estudio tuvo como objetivo determinar la presencia de anticuerpos contra Actinobaccilus suis en credos provenientes de granjas con crianza tecnificada del norte, centro y sur del departamento de Lima. Un total de 474 muestras de sangre de porcinos aparentemente sanos, no vacunados contra A. suis, de 17 a 20 semanas de edad se obtuvieron al momento de su beneficio. La evaluación serológica se realizó mediante la prueba de aglutinación en placa utilizando un antígeno específico contra A. suis empleando tres diluciones (1/40, 1/80 y 1/160) y una cantidad constante de antígeno (0.03 ml.) se consideraron positivos los sueros que aglutinaron en las tres diluciones. Se observó la presencia de reactores contra a. suis en 142 de las 474 muestras, determinándose una prevalencia del 30% con una mayor frecuencia de sueros positivos en las zonas sur y norte de Lima.
