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1.
Photochem Photobiol Sci ; 20(3): 327-341, 2021 Mar.
Article in English | MEDLINE | ID: mdl-33721278

ABSTRACT

A novel amphiphilic photosensitizing agent based on a tricationic fullerene C60 (DMC603+) was efficiently synthesized from its non-charged analogue MMC60. These fullerenes presented strong UV absorptions, with a broad range of less intense absorption up to 710 nm. Both compounds showed low fluorescence emission and were able to photosensitize the production of reactive oxygen species. Furthermore, photodecomposition of L-tryptophan sensitized by both fullerenes indicated an involvement of type II pathway. DMC603+ was an effective agent to produce the photodynamic inactivation (PDI) of Staphylococcus aureus, Escherichia coli and Candida albicans. Mechanistic insight indicated that the photodynamic action sensitized by DMC603+ was mainly mediated by both photoprocesses in bacteria, while a greater preponderance of the type II pathway was found in C. albicans. In presence of potassium iodide, a potentiation of PDI was observed due to the formation of reactive iodine species. Therefore, the amphiphilic DMC603+ can be used as an effective potential broad-spectrum antimicrobial photosensitizer.


Subject(s)
Anti-Infective Agents/chemistry , Fullerenes/chemistry , Photosensitizing Agents/chemistry , Potassium Iodide/chemistry , Anti-Infective Agents/chemical synthesis , Anti-Infective Agents/pharmacology , Candida albicans/drug effects , Cations/chemistry , Density Functional Theory , Escherichia coli/drug effects , Kinetics , Light , Oxidation-Reduction , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/pharmacology , Reactive Oxygen Species/metabolism , Staphylococcus aureus/drug effects
2.
Photodiagnosis Photodyn Ther ; 31: 101877, 2020 Sep.
Article in English | MEDLINE | ID: mdl-32534247

ABSTRACT

Photocytotoxic effect induced by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (TAPP) and 5,10,15,20-tetrakis[4-(3-N,N,N-trimethylaminepropoxy)phenyl]porphyrin (TAPP+4) was examined in Candida albicans to obtain information on the mechanism of photodynamic action and cell damage. For this purpose, the photokilling of the yeast was investigated under anoxic conditions and cell suspensions in D2O. Moreover, photoinactivation of C. albicans was evaluated in presence of reactive oxygen species scavengers, such as sodium azide and d-mannitol. The results indicated that singlet molecular oxygen was the main reactive species involved in cell damage. On the other hand, the binding and distribution of these porphyrins in the cells was observed by fluorescence microscopy. Morphological damage was studied by transmission electron microscopy (TEM), indicating modifications in the cell envelopment. Furthermore, deformed cells were observed after photoinactivation of C. albicans by toluidine blue staining. In addition, modifications in the cell envelope due to the photodynamic activity was found by scanning electron microscopy (SEM). Similar photodamage was observed with both porphyrin, which mainly produced alterations in the cell barriers that lead to the photoinactivation of C. albicans.


Subject(s)
Photochemotherapy , Porphyrins , Candida albicans , Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Porphyrins/pharmacology , Singlet Oxygen
3.
Photodiagnosis Photodyn Ther ; 24: 220-227, 2018 Dec.
Article in English | MEDLINE | ID: mdl-30278279

ABSTRACT

New porphyrin derivatives bearing basic aliphatic amino groups were synthesized from the condensation of meso-4-[(3-N,N-dimethylaminopropoxy)phenyl]dipyrromethane, pentafluorobenzaldehyde and 4-(3-N,N-dimethylaminopropoxy)benzaldehyde. The reaction was catalyzed by trifluoroacetic acid in acetonitrile. This approach was used to obtain porphyrins with different patterns of substitution, of which three of them were isolated: 5,15-di(4-pentafluorophenyl)-10,20-di[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (F10APP), 5-(4-pentafluorophenyl)-10,15,20-tris[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (F5APP) and 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (TAPP). The UV-vis spectroscopic characterizations and the photodynamic effect of these compounds were compared in N,N-dimethylformamide. These porphyrins showed red fluorescence emission with quantum yields of 0.09-0.15. Moreover, they sensitized the production of singlet molecular oxygen, reaching quantum yields values of 0.33-0.53. Photodynamic inactivation was studied in two bacteria, Staphylococcus aureus and Escherichia coli, and a yeast Candida albicans. High amount of cell-bound porphyrin was obtained at short times (<2 min) of incubation. After 15 min irradiation, a 7 log reduction of S. aureus was found for cells treated with 1 µM F5APP. Similar photokilling was obtained in E. coli, but using 7.5 µM F5APP and 30 min irradiation. Under these conditions, a decrease of 5 log was observed in C. albicans cells. An increase in cell survival was observed by addition of sodium azide, whereas a slight protective effect was found in the presence of D-mannitol. Moreover, the photoinactivation mediated by these porphyrins was higher in D2O than in water. Thus, these porphyrins induced the photodynamic activity mainly through the intermediacy of O2(1Δg). In particular, F5APP was a highly effective photosensitizer with application as a broad-spectrum antimicrobial. This porphyrin contains three basic aliphatic amino groups that may be protonated at physiological pH. In addition, it is substituted by a lipophilic pentafluorophenyl group, which confers an amphiphilic character to the tetrapyrrolic macrocycle. This effect can increase the interaction with the cell envelopment, improving the photocytotoxic activity against the microorganisms.


Subject(s)
Benzaldehydes/pharmacology , Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Porphyrins/pharmacology , Amines/chemistry , Amines/pharmacology , Benzaldehydes/chemistry , Candida albicans/drug effects , Escherichia coli/drug effects , Hydrogen-Ion Concentration , Porphyrins/chemistry , Staphylococcus aureus/drug effects
4.
Photodiagnosis Photodyn Ther ; 23: 261-269, 2018 Sep.
Article in English | MEDLINE | ID: mdl-29964223

ABSTRACT

Silica nanoparticles (SiNPs) embedded with Zn(II) 2,9,16,23-tetrakis(methoxy)phthalocyanine (SiNPZnPcOCH3), Zn(II) 2,9,16,23-tetrakis(4-pyridyloxy) phthalocyanine (SiNPZnPcOPy) and Zn(II) 2,9,16,23-tetrakis(t-butyl) phthalocyanine (SiNPZnPctBu) were synthesized in the nonpolar core of AOT/1-butanol/water micelles using triethoxyvinylsilane and 3-aminopropyltriethoxysilane. These SiNPs-Pc presented an average diameter of about 20-25 nm. UV-vis absorption spectra presented the characteristic Soret and Q bands of phthalocyanines embedded into the nanoparticles. Moreover, red fluorescence emission of SiNPs bearing phthalocyanines was detected in water. The SiNPs-Pc produced the photodecomposition of 2,2'-(anthracene-9,10-diyl)bis(methylmalonic acid), which was used to sense the singlet molecular oxygen O2(1Δg) generation in aqueous medium. Also, the formation of superoxide anion radical was detected by nitro blue tetrazolium reduction in the presence of NADH. Photoinactivation of microorganisms was investigated in Staphylococcus aureus and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by SiNPZnPcOCH3 and SiNPZnPctBu improved with an increase of irradiation times. After 30 min irradiation, over 7 log reduction was found for S. aureus. Also, these SiNPs-Pc produced a decrease of 2.5 log in C. albicans after 60 min irradiation. In both cases, a lower photoinactivation activity was found for SiNPZnPcOPy. Studies of photodynamic action mechanism showed that the photokilling of microbial cells was protected in the presence of sodium azide and diazabicyclo[2.2.2]octane. Also, a reduction on the cell photodamage was found with the addition of D-mannitol. Therefore, the photodynamic activity sensitized by SiNPZnPcOCH3 and SiNPZnPctBu in microbial cells was mediated by a contribution of both type I and type II photooxidative mechanisms. Thus, silica nanoparticles are interesting materials to vehicle ZnPcOCH3 and ZnPctBu in aqueous media to photoeradicate microorganisms.


Subject(s)
Indoles/pharmacology , Nanoparticles/chemistry , Photosensitizing Agents/pharmacology , Silicon Dioxide/chemistry , Candida albicans/drug effects , Drug Delivery Systems , Escherichia coli/drug effects , Indoles/administration & dosage , Indoles/analysis , Isoindoles , Particle Size , Photochemotherapy , Photosensitizing Agents/administration & dosage , Singlet Oxygen/metabolism , Staphylococcus aureus/drug effects , Superoxides/metabolism
5.
J Photochem Photobiol B ; 158: 243-51, 2016 May.
Article in English | MEDLINE | ID: mdl-26994333

ABSTRACT

A novel 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was synthesized by reduction of the corresponding porphyrin TAPP with p-toluenesulfonhydrazide, followed by selective oxidation with o-chloranil. Spectroscopic properties and the photodynamic activity of these photosensitizers were compared in N,N-dimethylformamide. An increase in the absorption band at 650nm was found for the chlorin derivative with respect to TAPP. These photosensitizers emit red fluorescence with quantum yields of 0.15. Both compounds were able to photosensitize singlet molecular oxygen with quantum yields of about 0.5. Also, the formation of superoxide anion radical was detected in the presence of TAPC or TAPP and NADH. Photodynamic inactivation was investigated on a Gram-positive bacterium Staphylococcus aureus, a Gram-negative bacterium Escherichia coli and a fungal yeast Candida albicans cells. In vitro experiments showed that TAPC or TAPP were rapidly bound to microbial cells at short incubation periods. These photosensitizers, without intrinsic positive charges, contain four basic amino groups. These substituents can be protonated at physiological pH, increasing the interaction with the cell envelopment. Photosensitized inactivation improved with an increase of both photosensitizer concentrations and irradiation times. After 15min irradiation, a 7 log reduction of S. aureus was found for treated with 1µM photosensitizer. Similar result was obtained with E. coli after using 5µM photosensitizer and 30min irradiation. Also, the last conditions produced a decrease of 5 log in C. albicans cells. Therefore, TAPC was highly effective as a broad-spectrum antimicrobial photosensitizer.


Subject(s)
Anti-Infective Agents/pharmacology , Photosensitizing Agents/pharmacology , Porphyrins/chemical synthesis , Porphyrins/pharmacology , Magnetic Resonance Spectroscopy , Porphyrins/chemistry , Spectrometry, Fluorescence/methods , Spectrophotometry, Ultraviolet/methods
6.
Photodiagnosis Photodyn Ther ; 13: 334-340, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26498876

ABSTRACT

The photodynamic inactivation mediated by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (TAPP) and 5,10,15,20-tetrakis[4-(3-N,N,N-trimethylaminepropoxy)phenyl]porphyrin (TAPP(4+)) were compared in Candida albicans cells. A strong binding affinity was found between these porphyrins and the yeast cells. Photosensitized inactivation of C. albicans increased with both photosensitizer concentration and irradiation time. After 30 min irradiation, a high photoinactivation (∼5 log) was found for C. albicans treated with 5 µM porphyrin. Also, the photoinactivation of yeast cells was still elevated after two washing steps. However, the photocytotoxicity decreases with an increase in the cell density from 10(6) to 10(8) cells/mL. The high photodynamic activity of these porphyrins was also established by growth delay experiments. This C. albicans strain was susceptible to fluconazole with a MIC of 1.0 µg/mL. The effect of photosensitization and the action of fluconazole were combined to eradicate C. albicans. After a PDI treatment with 1 µM porphyrin and 30 min irradiation, the value of MIC decreased to 0.25 µg/mL. In addition, a complete arrest in cell growth was found by combining both effects. TAPP was similarly effective to photoinactivate C. albicans than TAPP(4+). This porphyrin without intrinsic positive charges contains basic amino groups, which can be protonated at physiological pH. Moreover, an enhancement in the antifungal action was found using both therapies because lower doses of the agents were required to achieve cell death.


Subject(s)
Candida albicans/drug effects , Candida albicans/physiology , Photochemotherapy/methods , Porphyrins/administration & dosage , Porphyrins/chemistry , Sterilization/methods , Candida albicans/chemistry , Cations , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Hydrogen-Ion Concentration , Microbial Viability/drug effects , Microbial Viability/radiation effects , Photosensitizing Agents/administration & dosage , Photosensitizing Agents/chemistry , Treatment Outcome
7.
Photodiagnosis Photodyn Ther ; 11(2): 148-55, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24642192

ABSTRACT

The photodynamic activity of brominated derivatives of New Fuchsin and Azure B was studied in solution and in cell suspensions of Candida albicans. The spectroscopic and photodynamic properties of these photosensitizers were compared with those of Crystal Violet and Azure B, which represent active photosensitizer related to each family of compounds. Triarylmethane derivatives absorb intensely with a band centered at ∼ 570 nm, while the phenothiazinium dyes at ∼ 650 nm. Photooxidation of 9,10-dimethylanthracene was observed using phenothiazinium compounds indicating the formation of singlet molecular oxygen, while it was not detected using triarylmethane agents. However, triarylmethane dyes were able to photooxidize l-tryptophan. In yeast cell suspensions, the photosensitized inactivation of C. albicans increases with photosensitizer concentration, causing a ∼ 5 log decrease of cell survival, when the cultures are treated with 20 µM of Crystal Violet and irradiated for 60 min. Under these conditions, the photodynamic activity of 50 µM Azure B induced a ∼ 3 log decrease of cell survival. Studies of photodynamic action mechanism indicated that photoinactivation of C. albicans cells induced by triarylmethane compounds involves mainly type I photoprocess. Although, phenothiazinium derivatives produce singlet molecular oxygen, a contribution of other reactive oxygen species cannot be discarded in the photoinactivation of C. albicans.


Subject(s)
Bromine/chemistry , Candida albicans/physiology , Methane/chemistry , Phenothiazines/chemistry , Photosensitizing Agents/administration & dosage , Photosensitizing Agents/chemical synthesis , Apoptosis/drug effects , Apoptosis/radiation effects , Bromine/administration & dosage , Candida albicans/drug effects , Candida albicans/radiation effects , Cell Proliferation/drug effects , Cell Proliferation/radiation effects , Light , Methane/administration & dosage , Phenothiazines/administration & dosage
8.
Eur J Med Chem ; 58: 332-9, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23142673

ABSTRACT

Photodynamic inactivation of Candida albicans produced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP(3+)), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP(4+)) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP(4+)) was investigated to obtain insight about the mechanism of cellular damage. In solution, absorption spectroscopic studies showed that these cationic porphyrins interact strongly with calf thymus DNA. The electrophoretic analysis indicated that photocleavage of DNA induced by TFAP(3+) took place after long irradiation periods (>5 h). In contrast, TMAP(4+) produced a marked reduction in DNA band after 1 h irradiation. In C. albicans, these cationic porphyrins produced a ∼3.5 log decrease in survival when the cell suspensions (10(7) cells/mL) were incubated with 5 µM photosensitizer and irradiated for 30 min with visible light (fluence 162 J/cm(2)). After this treatment, modifications of genomic DNA isolated from C. albicans cells were not found by electrophoresis. Furthermore, transmission electron microscopy showed structural changes with appearance of low density areas into the cells and irregularities in cell barriers. However, the photodamage to the cell envelope was insufficient to cause the release of intracellular biopolymers. Therefore, modifications in the cytoplasmic biomolecules and alteration in the cell barriers could be mainly involved in C. albicans photoinactivation.


Subject(s)
Candida albicans/drug effects , Light , Photosensitizing Agents/pharmacology , Porphyrins/pharmacology , Animals , Candida albicans/cytology , Candida albicans/genetics , Cations/chemical synthesis , Cations/chemistry , Cations/pharmacology , Cattle , DNA/chemistry , DNA, Fungal/genetics , DNA, Fungal/isolation & purification , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/chemistry , Porphyrins/chemical synthesis , Porphyrins/chemistry , Structure-Activity Relationship
9.
Bioorg Med Chem ; 20(13): 4032-9, 2012 Jul 01.
Article in English | MEDLINE | ID: mdl-22672980

ABSTRACT

Novel photoactive bridged polysilsesquioxane films were prepared by doped with a porphyrin derivative. The films were formed by acid-catalyzed polycondensation reaction of a precursor of a bridged silsesquioxane, based on the reaction product of (glycidoxypropyl)trimethoxysilane with n-dodecylamine in the presence of 5-(4-carboxyphenyl)-10,15,20-tris(4-methylphenyl)porphyrin, followed by solvent evaporation. This procedure allowed obtaining flexible thin films. Absorption and fluorescence spectroscopic analysis showed the characteristic bands of the porphyrin in the visible region indicating that the photosensitizer is mainly embedded as monomer in the films. Photodynamic properties of the polymeric films were studied in solution containing photooxidizable substrates. Singlet molecular oxygen, O(2)((1)Δ(g)), production was observed by the reaction with 9,10-dimethylanthracene and 9,10-anthracenediyl-bis(methylene)dimalonic acid in different media. Also, these films photosensitized the decomposition of l-tryptophan. In vitro investigations showed that these films produce photodynamic inactivation of Candida albicans cells in aqueous suspensions and on their surfaces. These films exhibit a photosensitizing activity causing a ∼2.5 log (99.7%) decrease of cellular survival after 60 min of irradiation with visible light. Also, the photocytotoxicity of the surfaces was tested under condition of microbial growth. Yeast cells exposed to the film and illuminated showed growth delay compared with controls. Studies of photodynamic action mechanism showed that the photoinactivation increased in D(2)O, while cells were protected in the presence of azide ion. In contrast, the addition of mannitol produced a negligible effect on the cellular phototoxicity. These results provide evidence that O(2)((1)Δ(g)) produced by the polymeric film doped with porphyrin can successfully inactivate C. albicans in cell suspensions and deposited on the film surface.


Subject(s)
Candida albicans/drug effects , Organosilicon Compounds/chemistry , Photosensitizing Agents/chemistry , Porphyrins/chemistry , Absorption , Acids/chemistry , Anthracenes/chemistry , Candida albicans/radiation effects , Catalysis , Kinetics , Light , Oxidation-Reduction , Photolysis , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/pharmacology , Singlet Oxygen/metabolism , Spectrometry, Fluorescence
10.
Photochem Photobiol Sci ; 10(10): 1556-61, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21748182

ABSTRACT

The photodynamic mechanism of action induced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP(3+)), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP(4+)) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP(4+)) was investigated on Candida albicans cells. These cationic porphyrins are effective photosensitizers, producing a ~5 log decrease of cell survival when the cultures are incubated with 5 µM photosensitizer and irradiated for 30 min with visible light. Studies under anoxic conditions indicated that oxygen is necessary for the mechanism of action of photodynamic inactivation of this yeast. Furthermore, photoinactivation of C. albicans cells was negligible in the presence of 100 mM azide ion, whereas the photocytotoxicity induced by these porphyrins increased in D(2)O. In contrast, the addition of 100 mM mannitol produced a negligible effect on the cellular phototoxicity. On the other hand, in vitro direct observation of singlet molecular oxygen, O(2)((1)Δ(g)) phosphorescence at 1270 nm was analyzed using C. albicans in D(2)O. A shorter lifetime of O(2)((1)Δ(g)) was found in yeast cellular suspensions. These cationic porphyrins bind strongly to C. albicans cells and the O(2)((1)Δ(g)) generated inside the cells is rapidly quenched by the biomolecules of the cellular microenvironment. Therefore, the results indicate that these cationic porphyrins appear to act as photosensitizers mainly via the intermediacy of O(2)((1)Δ(g)).


Subject(s)
Candida albicans/drug effects , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Porphyrins/chemistry , Quaternary Ammonium Compounds/chemistry , Azides/chemistry , Deuterium Oxide/chemistry , Light , Mannitol/chemistry , Oxygen/chemistry , Porphyrins/pharmacology , Quaternary Ammonium Compounds/pharmacology , Singlet Oxygen/metabolism
11.
Environ Sci Technol ; 43(3): 902-8, 2009 Feb 01.
Article in English | MEDLINE | ID: mdl-19245034

ABSTRACT

Spectroscopic and photodynamic properties of polymeric films bearing porphyrin units have been studied in both solution containing photooxidizable substrates and in vitro on Escherichia coli and Candida albicans microorganisms. The films were formed by electrochemical polymerization of 5,10,15,20-tetra(4-N,N-diphenylaminophenyl)porphyrin (H2P-film) and its complex with Pd(II) (PdP-film) on optically transparent indium tin oxide (ITO) electrodes. Absorption spectroscopic studies show the characteristic Soret and Q bands of the porphyrin in the visible region and a band at approximately 350 nm corresponding to the tetraphenylbenzidine units. Upon excitation, the H2P-film exhibits two bands of fluorescence emission from porphyrin, while it is not detected using PdP-film. The singlet molecular oxygen, O2(1Deltag), productions of these surfaces were evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide. Also, the photodynamic activity was compared in solutions of L-tryptophan. Under these conditions, oxidation of these substrates takes place indicating an efficient photodynamic action of both polymeric films. In vitro investigations show that these films produce photosensitized inactivation of microbial cells in aqueous suspensions. These films exhibit a photosensitizing activity causing a approximately 3 log decrease of E. coil and approximately 2.5 log of C. albicans cellular survival after 30 min of irradiation with visible light. The photodynamic effect of the surfaces was also tested by growth delay experiments. The results indicate that porphyrins immobilized on electropolymeric films are interesting and versatile photodynamic surfaces to inactivate microorganisms in liquid suspensions.


Subject(s)
Anti-Infective Agents/pharmacology , Polymers/chemistry , Porphyrins/chemistry , Candida albicans/drug effects , Candida albicans/growth & development , Electrochemistry , Escherichia coli/drug effects , Escherichia coli/growth & development , Photochemistry , Polymers/pharmacology , Porphyrins/pharmacology
12.
Eur J Med Chem ; 44(4): 1592-9, 2009 Apr.
Article in English | MEDLINE | ID: mdl-18762356

ABSTRACT

The photodynamic action of 5-(4-trifluorophenyl)-10,15,20-tris(4-trimethylammoniumphenyl)porphyrin iodide (TFAP(3+)) and 5,10,15,20-tetra(4-N,N,N-trimethylammonium phenyl)porphyrin p-tosylate (TMAP(4+)) has been studied in vitro on Candida albicans. The results of these cationic porphyrins were compared with those of 5,10,15,20-tetra(4-sulphonatophenyl)porphyrin (TPPS(4-)), which characterizes an anionic sensitizer. In vitro investigations show that these cationic porphyrins are rapidly bound to C. albicans cells, reaching a value of approximately 1.4 nmol/10(6) cells, when the cellular suspensions were incubated with 5 microM sensitizer for 30 min. In contrast, TPPS(4-) is poorly uptaken by yeast cells. The fluorescence spectra of these sensitizers into the cells confirm this behaviour. The amount of porphyrin binds to cells is dependent on both sensitizer concentrations (1-5 microM) and cells densities (10(6)-10(8) cells/mL). Photosensitized inactivation of C. albicans cellular suspensions increases with sensitizer concentration, causing a approximately 5 log decrease of cell survival, when the cultures are treated with 5 microM of cationic porphyrin and irradiated for 30 min. However, the photocytotoxicity decreases with an increase in the cell density, according to its low binding to cells. Under these conditions, the photodynamic activity of TFAP(3+) is quite similar to that produced by TMAP(4+), whereas no important inactivation effect was found for TPPS(4)(-). The high photodynamic activity of cationic porphyrins was confirmed by growth delay experiments. Thus, C. albicans cell growth was not detected in the presence of 5 microM TFAP(3+). Photodynamic inactivation capacities of these sensitizers were also evaluated on C. albicans cells growing in colonies on agar surfaces. Cationic porphyrins produce a growth delay of C. albicans colonies and viability of cells was not observed after 3 h irradiation, indicating a complete inactivation of yeast cells. Therefore, these results indicate that these cationic porphyrins are interesting sensitizers for photodynamic inactivation of yeasts in liquid suspensions or in localized foci of infection.


Subject(s)
Candida albicans/drug effects , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Porphyrins/chemistry , Porphyrins/pharmacology , Absorption , Buffers , Candida albicans/cytology , Candida albicans/metabolism , Candida albicans/radiation effects , Candidiasis/drug therapy , Cell Proliferation/drug effects , Cells, Immobilized/cytology , Cells, Immobilized/drug effects , Culture Media , Light , Photochemical Processes , Photosensitizing Agents/metabolism , Photosensitizing Agents/therapeutic use , Porphyrins/metabolism , Porphyrins/therapeutic use , Spectrometry, Fluorescence
13.
Photochem Photobiol Sci ; 7(8): 963-72, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18688504

ABSTRACT

The photophysical properties and photodynamic effect of Zn(ii), Pd(ii), Cu(ii) and free-base 5-(4-(trimethylammonium)phenyl)-10,15,20-tris(2,4,6-trimethoxy phenyl)porphyrin (H2P) iodide have been studied in N,N-dimethylformamide (DMF) and in different biomimetic systems. The absorption, fluorescence, triplet state and singlet molecular oxygen production of the metal complexes were all referred to H2P. The photodynamic activity was first analyzed using 9,10-dimethylanthracene and guanosine 5'-monophosphate in N,N-dimethylformamide. The photooxidation processes were also investigated in benzene/benzyl-n-hexadecyldimethyl ammonium chloride/water reverse micelles. Photosensitization efficiency of these porphyrins was H2P approximately ZnP > PdP in homogeneous solution and ZnP > H2P > PdP in micelles, whereas no photooxidation effect was detected using the Cu(ii) complex. Human erythrocytes were used as a biological membrane model. The photohemolytic activity depended on irradiation time, sensitizer and concentration of the agent. When cells were treated with 1 microM sensitizer, the hemolytic activity was H2P > ZnP >> CuP. However, it was H2P > ZnP approximately CuP using 5 microM of the respective porphyrin. Although CuP could undergo a type I photoreaction, in all cases the photohemolytic effect considerably diminishes in anoxic conditions, indicating that an oxygen atmosphere is required for the mechanism of cellular membrane damage. The behavior of these amphiphilic metallo porphyrins provides information on the photodynamic activity of these agents in biomimetic microenvironments.


Subject(s)
Light , Molecular Mimicry , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Porphyrins/chemistry , Porphyrins/pharmacology , Erythrocytes/drug effects , Hemolysis/drug effects , Humans , Micelles , Photochemotherapy , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet
14.
Int J Biochem Cell Biol ; 38(12): 2092-101, 2006.
Article in English | MEDLINE | ID: mdl-16899389

ABSTRACT

The photokilling activity of a porphyrin-C(60) (P-C(60)) dyad was evaluated on a Hep-2 human larynx-carcinoma cell line. This study represents the first evaluation of a dyad, with high capacity to form a photoinduced charge-separated state, to act as agent to inactivate cells by photodynamic therapy (PDT). Cell treatment was carried out with 1 microM P-C(60) incorporated into liposomal vesicles. No dark cytotoxicity was observed using 1 microM P-C(60) concentration and during long incubation time (24h). The uptake of sensitizer into Hep-2 was studied at different times of incubation. Under these conditions, a value of 1.5 nmol/10(6)cells was found after 4h of incubation showing practically no change even after 24h. The cell survival after irradiation of the cells with visible light was dependent upon light exposure level. A high photocytotoxic effect was observed for P-C(60), which inactivated 80% of the cells after 54 J/cm(2) of irradiation. Moreover, the dyad kept a high photoactivity even under argon atmosphere. Thus, depending on the microenvironment where the sensitizer is localized, this compound could produce a biological photodamage through either a (1)O(2)-mediated photoreaction process or a free radical mechanism under low oxygen concentration. The mechanism of cell death was analyzed by Hoechst-33258, toluidine blue staining, TUNEL and DNA fragmentation. Cell cultures treated for 24h with P-C(60) and irradiated with a dose of 54 J/cm(2) showed a great amount of apoptotic cells (58%). Moreover, changes in cell morphology were analyzed using fluorescence microscopy with Hoechst-33258 under low oxygen concentration. Under this anaerobic condition, necrotic cellular death predominated on apoptotic pathway. There were more apoptotic cells under air irradiation condition than under argon irradiation condition. To determine the apoptotic pathway, caspase-3 activation was studied by caspase-3 activity detection kits. The last results showed that P-C(60) induced apoptosis by caspase-3-dependent pathway. These results indicated that molecular dyad, which can form a photoinduced charge-separated state, is a promising model for phototherapeutic agents and they have potential application in cell inactivation by PDT.


Subject(s)
Laryngeal Neoplasms/pathology , Light , Photosensitizing Agents/chemistry , Photosensitizing Agents/pharmacology , Porphyrins/chemistry , Caspase 3/metabolism , Cell Death/drug effects , Cell Death/radiation effects , Cell Line , DNA, Neoplasm/metabolism , Darkness , Electrophoresis, Agar Gel , Enzyme Activation/drug effects , Enzyme Activation/radiation effects , Humans , Time Factors
15.
Int J Biochem Cell Biol ; 37(12): 2504-12, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16125432

ABSTRACT

The photokilling activity of 5-(4-trimethylammoniumphenyl)-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin (CP) was evaluated on a Hep-2 human larynx-carcinoma cell line. Cell treatment was carried out with 5 microM CP incorporated into liposomal vesicles. Under violet-blue exciting light, the red fluorescence of CP was mainly detected as a filamentous pattern characteristic of mitochondrial localization. Similar pattern was also observed using rhodamine 123 in Hep-2 cells. No dark cytotoxicity was observed using 5 microM CP concentration and long incubation time (24 h). Using Hoechst-33258 and caspase-3 immunostaining methods, cell cultures treated for 24 h with CP and exposed to light for 7.5 min (27 J/cm2) showed a great amount of apoptotic cells (40%). In contrast, necrotic cells (58%) were observed using the same drug concentration but irradiated for 15 min (54 J/cm2). The results show that CP can induce different mechanisms of cell death depending on irradiation doses in the photodynamic treatments, which makes this agent an interesting sensitizer with potential application in photodynamic tumor therapy.


Subject(s)
Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Porphyrins/therapeutic use , Apoptosis/drug effects , Carcinoma/drug therapy , Carcinoma/metabolism , Caspase 3 , Caspases/metabolism , Cell Death/drug effects , Cell Death/radiation effects , Cell Line, Tumor , Darkness , Enzyme Activation , Humans , Laryngeal Neoplasms/drug therapy , Laryngeal Neoplasms/metabolism , Liposomes , Microscopy, Fluorescence , Mitochondria/metabolism , Necrosis/chemically induced , Photosensitizing Agents/metabolism , Porphyrins/metabolism , Rhodamine 123/pharmacology
16.
Photochem Photobiol ; 81(4): 891-7, 2005.
Article in English | MEDLINE | ID: mdl-15757366

ABSTRACT

The photodynamic activities of a porphyrin-C60 dyad (P-C60) and its metal complex with Zn(II) (ZnP-C60) were compared with 5-(4-acetamidophenyl)-10,15,20-tris(4-methoxyphenyl)porphyrin (P), both in homogeneous medium-bearing photooxidizable substrates and in vitro on the Hep-2-human-larynx-carcinoma cell line. This study represents the first evaluation of dyads, with a high capacity to form a photoinduced charge-separated state, to act as agents to inactivate cells by photodynamic therapy (PDT). Absorption and fluorescence spectroscopic studies were performed in toluene and N,N-dimethylformamide (DMF). The emission of the porphyrin moiety in the dyads is strongly quenched by the attached fullerene C60 moiety. The singlet molecular oxygen, O2(1delta(g)), productions (phi(delta)) were determined using 9,10-dimethylanthracene (DMA). The values of phi(delta) were strongly dependent on the solvent's polarity. Comparable phi(delta) values were found for dyads and P in toluene, while O2(1delta(g)) production was significantly diminished for the dyads in DMF. In more polar solvent, the stabilization of charge-transfer state takes place, decreasing the efficiency of porphyrin triplet-state formation. Also, both dyads photosensitize the decomposition of L-tryptophan in DMF. In biological medium, no dark cytotoxicity was observed using sensitizer concentrations < or = 1 microM and 24 h of incubation. The uptake of sensitizers into Hep-2 was studied using 1 microM of sensitizer and different times of incubation. Under these conditions, a value of approximately 1.5 nmol/10(6) cells was found between 4 and 24 h of incubation. The cell survival after irradiation of the cells with visible light was dependent upon light-exposure level. A higher photocytotoxic effect was observed for P-C60, which inactivates 80% of cells after 15 min of irradiation. Moreover, both dyads keep a high photoactivity even under argon atmosphere. Thus, depending on the microenvironment where the sensitizer is localized, these compounds could produce biological photodamage through either an O2(1delta(g))-mediated photoreaction process or a free-radicals mechanism under low oxygen concentration. These results show that molecular dyads, which can form a photoinduced charge-separated state, are a promising model for phototherapeutic agents, with potential applications in cell inactivation by PDT.


Subject(s)
Fullerenes/chemistry , Photosensitizing Agents , Porphyrins/chemistry , Light , Photochemotherapy
17.
Photodiagnosis Photodyn Ther ; 1(4): 335-44, 2004 Dec.
Article in English | MEDLINE | ID: mdl-25048437

ABSTRACT

BACKGROUND AND OBJETIVE: Photodynamic therapy, a novel treatment for cancer, works through photoactivation of a tumor-localized photosensitive drug, and localized through oxidative damage to kill cells and ablate tumors. Pharmacokinetic and phototherapeutic properties of a cationic porphyrin were assayed in a Balb/c mouse cancer model in order to evaluate its efficiency as photosensitizer. METHODS: Biodistribution studies were carried out by intraperitoneal injection of 5mg/kg CP incorporated into a liposome solution. CP was recovered from serum and organs at various times after treatment. The serum biochemical parameters and histological studies were used to test hepatic and renal functionality. For phototherapeutic studies, the light source used was a slide projector (360J/cm(2)). The efficiency of CP was evaluated by following tumor growth curves for 10 days after PDT doses. Immunohistochemical detection was carried out to evaluate caspase-3 activation in CP-PDT-treated tumors. RESULTS AND CONCLUSIONS: The photosensitizer distribution suggests that CP is mainly eliminated from the organism via the bile-gut pathway, and that neurotoxic and cutaneous photosensitivity effects are reduced or absent. The porphyrin distribution from bloodstream to tissue began at 24h of drug administration. CP did not affect the hepatic and renal functionality, as was demonstrated by the physiological parameters. PDT-treated tumors showed delay in growth rate as compared to untreated control mice. Biochemical studies showed that the efficient tumor regression is dependent on caspase-3 activity signaling response associated with apoptosis. The results obtained suggest that the porphyrin CP may be a promising candidate for further use in PDT treatments.

18.
Photochem Photobiol Sci ; 2(9): 926-33, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14560810

ABSTRACT

A novel 5-[4-(trimethylammonium)phenyl]-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin iodide (2) has been synthesized. A positive charge was incorporated at a peripheral position to increase the amphiphilic character of the structure. The photodynamic effect of the cationic porphyrin 2 was compared with that of non-charged 5-(4-aminophenyl)-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin (1), both in a homogeneous medium bearing photooxidizable substrates and in vitro on the Hep-2 human larynx carcinoma cell line. Absorption and fluorescence spectroscopic studies in different media show that 2 is essentially unaggregated in solution, and also in human cells. The singlet molecular oxygen, O2(1delta(g)), production was evaluated using 9,10-dimethylanthracene in N,N-dimethylformamide, yielding phi(delta) values of approximately 0.66 for both porphyrins. The addition of beta-carotene suppresses the O2(1delta(g))-mediated photooxidation. L-Tryptophan and guanosine 5'-monophosphate were used as biological substrate models. Porphyrin 2 sensitizes the decomposition of both compounds faster than does 1. In the biological medium, no dark cytotoxicity was observed, even though a high porphyrin concentration (10 microM) and a long incubation time (24 h) were employed. Cell treatments were performed with 5 microM of porphyrin for 24 h. Under these conditions, the uptake of porphyrin 2 into Hep-2 was about 3 times higher than that of 1. Cell survival after irradiation with visible light was dependent upon both the light exposure level and intracellular sensitizer concentration. Thus, a higher photocytotoxic effect was found for porphyrin 2 in comparison to 1. These results show that the amphiphilic monocationic porphyrin 2 could be a promising model for phototherapeutic agents with potential applications in tumor cell inactivation by photodynamic therapy.


Subject(s)
Porphyrins/pharmacology , Anthracenes/chemistry , Cations , Guanosine Monophosphate/chemistry , Humans , Mass Spectrometry , Oxidation-Reduction , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Tryptophan/chemistry , Tumor Cells, Cultured , beta Carotene/pharmacology
19.
Biomed Pharmacother ; 57(3-4): 163-8, 2003.
Article in English | MEDLINE | ID: mdl-12818478

ABSTRACT

The photokilling activity of 5-[4-N-(2',6'-dinitro-4'-trifluoromethylphenyl) aminophenyl]-10,15,20-tris(2,4,6-trimethoxy phenyl) porphyrin (CF3) was evaluated on a Hep-2 human larynx-carcinoma cell line. An apoptotic mechanism of cell death was found at low irradiation doses. Pharmacokinetic and tumour-photosensitizing properties were studied in mice. The results show that a different mechanism of cell death can be induced depending on the irradiation doses in the photodynamic treatments with CF3, which makes this agent an interesting sensitizer for potential tumour photosensitizer.


Subject(s)
Phloroglucinol/analogs & derivatives , Phloroglucinol/pharmacology , Phloroglucinol/pharmacokinetics , Photosensitizing Agents/pharmacology , Photosensitizing Agents/pharmacokinetics , Porphyrins/pharmacology , Porphyrins/pharmacokinetics , Animals , Cell Count , Cell Line, Tumor , Cell Survival/drug effects , Cell Survival/radiation effects , Female , Humans , Liposomes , Mice , Mice, Inbred BALB C , Photic Stimulation , Photochemotherapy , Tissue Distribution
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