ABSTRACT
The effects of chronic ischemia on the rat hepatocyte are morphometrically analyzed in the present study. Rats Wistar-Lewis are used. Rats underwent a stenosis of the celiac artery by using a metallic guide. The interval between the experimentation until the sacrifice was 15, 30, 90, 180, and 360 days. We have performed a morphometric study using a semiautomated image analyzer system (VIDS III, Analytical Measuring Systems). Morphological disturbances in the chronic ischemia periods above mentioned can be measured: an increase in the hepatocyte area starting from 90 days of experimentation and also an increase in the hepatocyte perimeter starting from 30 days of ischemia. On the other hand, both the nuclear area and the nuclear perimeter are increased in every period studied. The number of hepatocytes per microscopic field doesn't show any change in relation to ischemia time. Cellular swelling suggests some degree of cellular hypoxia in contradiction with other observations.
Subject(s)
Ischemia/pathology , Liver/blood supply , Liver/pathology , Animals , Chronic Disease , Disease Models, Animal , Male , Rats , Rats, Wistar , Statistics as TopicABSTRACT
The rate of patients with extrapulmonary tuberculosis has increased in the last years (about 5% of all cases), and displaying a wide spectrum of clinical manifestations. Among them, the anal localization is a rare occurrence (0.7%) according to available published data. During the last two years, we have diagnosed and treated three patients with perianal tuberculous lesions manifested by ulcers. All three were males, associated with active pulmonary tuberculosis, and one with intestinal involvement which required surgical treatment. Based on this experience, we have reviewed the different etiopathogenic, clinical, diagnostic and therapeutic manifestations of this picture, emphasizing the importance of suspicion, and its diagnostic easiness if the different infectious and inflammatory diseases at this level are taken into account.
