Subject(s)
Angioedemas, Hereditary/complications , Factor XII Deficiency/complications , Hemorrhage/etiology , Hemorrhage/prevention & control , Heparin, Low-Molecular-Weight/therapeutic use , Pregnancy Complications , Tranexamic Acid/therapeutic use , Angioedemas, Hereditary/etiology , Drug Therapy, Combination , Female , Heparin, Low-Molecular-Weight/administration & dosage , Humans , Pregnancy , Tranexamic Acid/administration & dosageABSTRACT
No disponible
Subject(s)
Humans , Female , Pregnancy , Adult , Tranexamic Acid , Antifibrinolytic Agents/administration & dosage , Heparin, Low-Molecular-Weight/administration & dosage , Angioedemas, Hereditary/drug therapy , Pregnancy Complications/drug therapy , Treatment OutcomeABSTRACT
BACKGROUND: Little is known about risk factors for complications in chronic pancreatitis (CP). High fat diet (HFD) has been demonstrated to aggravate pancreatic injury in animal models. The aim of this study was to investigate the role of HFD in age at diagnosis of CP and probability of CP related complications. METHODS: A cross-sectional case-case study was performed within a prospectively collected cohort of patients with CP. Diagnosis and morphological severity of CP was established by endoscopic ultrasound. Pancreatic exocrine insufficiency (PEI) was diagnosed by ¹³C mixed triglyceride breath test. Fat intake was assessed by a specific nutritional questionnaire. Odds ratios (OR) for CP related complications were estimated by multivariate logistic regression analysis. RESULTS: 168 patients were included (128 (76.2%) men, mean age 44 years (SD 13.5)). Etiology of CP was alcohol abuse in 89 patients (53.0%), other causes in 30 (17.9%) and idiopathic in the remaining 49 subjects (29.2%). 24 patients (14.3%) had a HFD. 68 patients (40.5%) had continuous abdominal pain, 39 (23.2%) PEI and 43 (25.7%) morphologically severe CP. HFD was associated with an increased probability for continuous abdominal pain (OR = 2.84 (95% CI, 1.06-7.61)), and a younger age at diagnosis (37.0 ± 13.9 versus 45.8 ± 13.0 years, p = 0.03) but not with CP related complications after adjusting for sex, years of follow-up, alcohol and tobacco consumption, etiology and body mass index. CONCLUSIONS: Compared with a normal fat diet, HFD is associated with a younger age at diagnosis of CP and continuous abdominal pain, but not with severity and complications of the disease.
Subject(s)
Abdominal Pain/etiology , Diet, High-Fat/adverse effects , Pancreas, Exocrine/physiopathology , Pancreatitis, Chronic/physiopathology , Abdominal Pain/epidemiology , Abdominal Pain/prevention & control , Adult , Age of Onset , Alcoholism/physiopathology , Case-Control Studies , Cohort Studies , Cross-Sectional Studies , Female , Hospitals, University , Humans , Male , Middle Aged , Pancreas, Exocrine/diagnostic imaging , Pancreatitis, Chronic/diet therapy , Pancreatitis, Chronic/epidemiology , Pancreatitis, Chronic/etiology , Prospective Studies , Risk Factors , Severity of Illness Index , Spain/epidemiology , Surveys and Questionnaires , UltrasonographyABSTRACT
Lactation in the rabbit is a nocturnal activity, extremely short and regular, that can be a strong synchronizer for the development of circadian rhythmicity in the pups. In the present study, 24-h rhythmicity of plasma prolactin and median eminence and anterior pituitary content of dopamine (DA), serotonin (5HT), gamma-aminobutyric acid (GABA) and taurine were examined in 11 days old female pups kept under 16 h light:8 h dark photoperiods (lights on at 08:00 h). Groups of six to seven female rabbit pups were killed by decapitation at six different time points throughout a 24-h cycle, starting at 09:00 h. Plasma prolactin levels changed significantly throughout the day, showing two peaks, one at first half of rest span (at 13:00 h) and another one at the beginning of the scotophase (at 01:00 h), just preceding doe visit. Median eminence DA content changed in a bimodal way as a function of time of day, displaying two maxima, at the beginning of the rest span and of the activity phase. Median eminence DA and plasma prolactin correlated significantly in an inverse way. Two maxima in median eminence 5HT levels were found, about 4 h in advance to the prolactin peaks. Circulating prolactin correlated inversely with median eminence 5HT content and directly with adenohypophysial 5HT content. Median eminence GABA content reached its maximum at the beginning of the scotophase and correlated significantly with plasma prolactin concentration. A positive correlation between plasma prolactin and adenohypophysial taurine content was observed. These results show that the circadian rhythmicity in prolactin secretory mechanisms in female rabbit pups develops during the early neonatal life.
Subject(s)
Aging/physiology , Circadian Rhythm/physiology , Median Eminence/metabolism , Pituitary Gland, Anterior/metabolism , Prolactin/blood , Rabbits/physiology , Animals , Animals, Newborn/blood , Animals, Newborn/metabolism , Dopamine/blood , Dopamine/metabolism , Female , Photoperiod , Rabbits/blood , Serotonin/blood , Serotonin/metabolism , Taurine/blood , Taurine/metabolism , Time Factors , gamma-Aminobutyric Acid/blood , gamma-Aminobutyric Acid/metabolismABSTRACT
The effects of ajoene (a potent antithrombotic agent obtained from garlic) on the tyrosine phosphorylation status of human platelet proteins were investigated by immunoblotting-based experiments using an anti-phosphotyrosine antibody. Incubation of platelets with ajoene enhanced the phosphorylation of at least four proteins (estimated MWs 76, 80, 84 and 120 kDa), both in resting platelets and in platelets subsequently stimulated with thrombin (0.1 U/ml). This effect was both dose- and incubation-time-dependent. High concentrations of ajoene (50 microM) or long periods of incubation (10 min) led to nonselective 'hyperphosphorylation' of numerous proteins. The effects of ajoene on protein tyrosine phosphatase (PTP) activity in platelet lysates were also investigated, PTP activity was inhibited when platelets were incubated with ajoene before lysis, but not when ajoene was added to lysates of platelets which had not been pre-exposed to ajoene.
