ABSTRACT
Optimal conditions for in vitro culture of feline ovarian follicles have not yet been defined. Follicular development is regulated by intraovarian growth factors, as insulin-like growth factor (IGF-1), and during the different stages of the oestrous cycle, follicles are exposed to specific hormonal environments. The aim of this study was to investigate the effect of IGF-1 on in vitro growth and granulosa cell (GC) viability of preantral follicles collected from domestic cats at follicular and luteal phases of the oestrous cycle. Oestrus and ovulation were induced in 12 cats. A total of 39 and 32 follicles collected at the follicular and luteal phases, respectively, were individually cultured in vitro for 6 days in minimum essential medium media supplemented with or without IGF-1 (100 ng/ml). Follicles collected during the follicular phase and cultured without IGF-1 displayed a significant increase in size and higher GC viability (46.5 ± 22.1 µm, 66.7%, respectively) than that of follicles collected at the luteal phase and cultured without IGF-1 (26.7 ± 14.4 µm, 50%, respectively; p < 0.05). In contrast, when IGF-1 was added to the culture medium, no differences were observed in size or GC viability between follicles collected at the two phases of the cycle. Nonetheless, follicles collected at the luteal phase and cultured with IGF-1 had a significant increase in their diameter and GC viability (31.9 ± 15.9 µm, 63.6%, respectively) than that cultured without IGF-1 (26.7 ± 14.4 µm, 50%, respectively; p < 0.05). These data suggest that in vitro growth and GC survival of feline preantral follicles are affected by the oestrous cycle phase, and the IGF-1 exerts a positive effect on follicles collected at the luteal phase.
Subject(s)
Cats/physiology , Culture Media/chemistry , Estrous Cycle/physiology , Insulin-Like Growth Factor I/pharmacology , Ovarian Follicle/drug effects , Tissue Culture Techniques/veterinary , Animals , Female , Ovarian Follicle/physiologyABSTRACT
Um modelo simplificado para deteccao de risco obstetrico foi testado no periodo de 1 de janeiro a 31 de dezembro de 1978 na Unidade Integrada de Saude de Sobradinho, pelo Setor de Obstetricia e Ginecologia da Universidade de Brasilia. Em 1980 procedeu-se a avaliacao utilizando uma amostragem de 500 casos selecionados aleatoriamente dentre as pacientes internadas no Hospital para atendimento obstetrico no ano de 1978. As variaveis de risco foram dicotomizadas pelo criterio de risco estatistico e risco por patologia. Na avaliacao, estas variaveis foram correlacionadas com os resultados desfavoraveis daquela gravidez. A sensibilidade de 77,4% e a especificidade de 79% foram julgadas muito boas. As variaveis estatisticas apontaram o risco com maior frequencia que as variaveis por patologia. Os autores comentam os resultados e consideram o modelo util para uso em niveis de atencao primaria a saude materno-infantil pela sua simplicidade e baixo custo
