ABSTRACT
Twenty-eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra-specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (P = 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh-2) were not in Hardy-Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.
Subject(s)
Candida albicans/enzymology , Candidiasis, Vulvovaginal/microbiology , Electrophoresis, Polyacrylamide Gel/methods , Adolescent , Adult , Alleles , Candida albicans/genetics , Candida albicans/isolation & purification , Cluster Analysis , Enzyme Activation , Enzyme Assays/methods , Female , Gene Frequency , Genetic Loci , Genetic Variation , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , L-Iditol 2-Dehydrogenase/genetics , L-Iditol 2-Dehydrogenase/metabolism , Malate Dehydrogenase/genetics , Malate Dehydrogenase/metabolism , Phenotype , Phospholipases/genetics , Phospholipases/metabolism , Young AdultABSTRACT
Objetivo: investigou-se a presença de Enterococcus faecalis em 25 dentes submetidos à retratamento endodôntico e em 10 casos com infecção endodôntica primária, ambos associados à periodontite apical assintomática. Realizou-se a coleta microbiológica e análise dos isolados clínicos por método de cultura tradicional e sistema automatizado. Dos 25 casos de retratamento, 18 apresentaram E. faecalis. Nos 10 casos de infecção primária, dois apresentaram o microrganismo. O percentual de ocorrência de E. faecalis foi mais elevado nos dentes submetidos à retratamento (72%) que nos casos de infecção endodôntica primária (20%). No entanto, para melhor entender o papel desse microganismo na infecção endodôntica, mais casos de infecção primária e de retratamento sem evidência de periodontite apical devem ser investigados, para que de fato se esclareça a importância do E. faecalis no insucesso endodôntico.
The aim of this study was investigate the presence of E. faecalis in samples obtained from 25 previously root-filled teeth and 10 cases of primary endodontic infection, both of which were asymptomatic apical periodontitis. Microbiological sampling was carried out, than the samples were analyzed by conventional culture methods and Micro-Scan Gram-positive identification panel. Results showed that of the 25 cases of previously root-filled teeth, E. faecalis occurred in 18, and in 10 cases of primary endodontic infection, E. faecalis occurred in two teeth. The data of this investigation showed a higher occurrence of E. faecalis in root-filled teeth (72%) compared with cases primary endodontic infection (20%). However, to better understanding the role of bacteria in endodontic infection, most cases of primary infection and re-treatment without evidence of apical periodontitis should be investigated, so that in fact makes clear the importance of E. faecalis in endodontic failure.
