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Sex Transm Dis ; 34(9): 709-17, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17414937

ABSTRACT

BACKGROUND: There is no data concerning genotyping of Chlamydia trachomatis from Brazilian samples. GOAL: To characterize the genotype of C. trachomatis detected in women assisted at a STD public clinic and establish the prevalence of this infection in that population. STUDY DESIGN: Endocervical samples of a group of 100 women were tested for chlamydial infection with PCR directed to C. trachomatis cryptic plasmid. Genotyping of positive samples were done after omp1 amplification and sequencing. RESULTS: The overall prevalence of C. trachomatis infection was 19%, with the highest prevalence in women between 15 and 25 years old (68.4%). Four genotypes were found associated with endocervical infections: D, E, F, and K. Sequence analysis revealed a coinfection of genotypes D and E in 1 woman. CONCLUSIONS: To our knowledge this is the first study to characterize Brazilian C. trachomatis endocervical samples and Brazilian C. trachomatis genotype coinfection. Our results also emphasize the importance of routine diagnosis of C. trachomatis for the control of this STD.


Subject(s)
Chlamydia Infections/epidemiology , Chlamydia Infections/microbiology , Chlamydia trachomatis/genetics , Adolescent , Adult , Base Sequence , Brazil/epidemiology , Chlamydia Infections/prevention & control , Chlamydia trachomatis/isolation & purification , DNA Primers , DNA, Bacterial/analysis , Female , Genotype , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Prevalence , Vaginal Smears
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